The bone regenerative effect of silk fibroin mixed with platelet-rich fibrin (PRF) in the calvaria defect of rabbit

Ji-Young Song; HaeYong Kweon; Kwang Jun Kwon; Young-Wook Park; Seong-Gon Kim

doi:10.5125/jkaoms.2010.36.4.250

Journal List > J Korean Assoc Oral Maxillofac Surg > v.36(4) > 1032396

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Song, Kweon, Kwon, Park, and Kim: The bone regenerative effect of silk fibroin mixed with platelet-rich fibrin (PRF) in the calvaria defect of rabbit

Original Article

J Korean Assoc Oral Maxillofac Surg 2010;36(4):250-254.

Published online: 10 January 2010

DOI: https://doi.org/10.5125/jkaoms.2010.36.4.250

The bone regenerative effect of silk fibroin mixed with platelet-rich fibrin (PRF) in the calvaria defect of rabbit

Ji-Young Song¹, HaeYong Kweon², Kwang Jun Kwon¹, Young-Wook Park¹, Seong-Gon Kim¹

¹Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung, Korea

²National Academy of Agricultural Science, RDA, Suwon, Korea

Seong-Gon Kim Department of Oral and Maxillofacial Surgery, Dental hospital, Gangneung-Woonju National University 120 Daehangno, Gangneung, 210-701, Korea Tel: +82-33-640-2468 Fax: +82-33-641-2477 E-mail: epken@chol.com

Received 13 April 2010 Revised 14 May 2010 Accepted 26 May 2010

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction

This study evaluated the bone regenerative effect of silk fibroin mixed with platelet-rich fibrin (PRF) of a bone defect in rabbits.

Materials and Methods

Ten New Zealand white rabbits were used for this study and bilateral round shaped defects were formed in the parietal bone (diameter: 8.0 mm). The silk fibroin mixed with PRF was grafted into the right parietal bone (experimental group). The left side (control group) was grafted only PRF. The animals were sacrificed at 4 weeks and 8 weeks. A micro-computerized tomography (μ CT) of each specimen was taken. Subsequently, the specimens were decalcified and stained for histological analysis.

Results

The average value of plane film analysis was higher in the experimental group than in the control group at 4 weeks and 8weeks after surgery. However, the difference was not statistically significant.(P>0.05) The tissue mineral density (TMD) in the experimental group at 4 weeks after surgery was significantly higher than the control group.(P<0.05)

Conclusion

Silk fibroin can be used as a scaffold of PRF for rabbit calvarial defect repair.

Keywords: Platelet-rich fibrin (PRF), Silk fibroin, Bone regeneration

References

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Fig. 1.

After centrifugation, the blood was separated in 3 layer. We used middle layer for grafting.

Fig. 2.

The silk protein mixed with PRF was grafted into the right parietal bone (experimental group: asterisk) and the left side (control group) was grafted only PRF. (PRF: platelet-rich fibrin)

Fig. 3.

Percent radiopacities within the PRF (test) and PRF+Silk (control) defects.

Fig. 4.

Histologic section (H&E staining, original magnification ×100). Osteoid formation both in the experimental group and in the control group at 4 weeks and 8 weeks.

Table 1.

Microscopic computerized tomography analysis

Sacrifice time	4 weeks			8 weeks
Group	PRF	PRF+SILK	P value¹	PRF	PRF+SILK	P value¹
BMC (mg)	365.61±25.10	361.92±25.96	0.19	419.52±20.89	414.32±30.53	0.34
BMD (mg/cc)	1217.63±86.38	1206.43±93.16	0.19	1394.11±70.99	1385.58±102.31	0.42
TMC (mg)	181.56±33.96	193.61±34.61	0.08	236.72±22.80	235.70±39.62	0.47
TMD (mg/cc)	2298.74±116.04	2376.26±135.72	0.04	2412.99±66.95	2425.66±86.76	0.15

¹ statistically significant P<0.05, BMC: bone mineral content, BMD: bone mineral density, TMC: tissue mineral content, TMD: tissue minera density)

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