Abstract
Purpose
Materials and Methods
Results
Figures and Tables
Fig. 2
In vivo standardization. Serially diluted allergen extracts were intradermally injected in subjects (A), and erythema size was measured after 15 to 20 minutes (B). The allergen potency was determined by calculating the dilution that induced an erythema diameter sum of 50 mm (C).
![ymj-57-399-g002](/upload/SynapseData/ArticleImage/0069ymj/ymj-57-399-g002.jpg)
Fig. 3
SDS-PAGE (A) and IgE immunoblot (B) analyses of Japanese hop pollen extracts. Twenty microlligrams of proteins were separated on 15% polyacrylamide gel under reducing conditions. IgE reactive proteins were probed with a pooled serum of Japanese hop-sensitized patients. M, molecular weight standards; A, commercial (allergopharma) Japanese hop extract; Y, our (Yonsei) collected Japanese hop extract. SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; IgE, immunoglobulin E.
![ymj-57-399-g003](/upload/SynapseData/ArticleImage/0069ymj/ymj-57-399-g003.jpg)
Fig. 4
SDS-PAGE (A) and IgE immunoblot (B) analyses of mugwort extracts. M, molecular mass marker; YS, Yonsei extract; HS, Hollister-Stier extract; A, mugwort-sensitized sera; B, non-atopic sera; C, buffer control.
![ymj-57-399-g004](/upload/SynapseData/ArticleImage/0069ymj/ymj-57-399-g004.jpg)
Fig. 5
ELISA inhibition analysis of Japanese hop extracts. IgE reactivity to our (Yonsei) collected (A) and commercial (Allergopharma) (B) extracts was inhibited at a various concentrations. ELISA, enzyme-linked immunosorbent assay.
![ymj-57-399-g005](/upload/SynapseData/ArticleImage/0069ymj/ymj-57-399-g005.jpg)
Fig. 6
In vitro standardization of mugwort extract. Competitive inhibition CAP was performed to estimate the potency of the extract using a pooled serum of eight subjects.
![ymj-57-399-g006](/upload/SynapseData/ArticleImage/0069ymj/ymj-57-399-g006.jpg)
Table 1
Clinical Features of the Enrolled Japanese Hop-Allergic Subjects
![ymj-57-399-i001](/upload/SynapseData/ArticleImage/0069ymj/ymj-57-399-i001.jpg)
AC, allergic conjunctivitis; AD, atopic dermatitis; AE, angioedema; AR, allergic rhinitis; AS, asthma; CC, chronic cough; CD, contact dermatitis; OAS, oral allergy syndrome; UT, urticaria; BAU, bioequivalent allergen unit; IgE, immunoglobulin E.
d1, Dermatophagoides pteronyssinus; d2, D. farinae; d72, Tyrophagus putrescentiae; i6, German cockroach; e2, dog hair; e5, dog dander; e6, Guinea pig epithelium; e82, rabbit epithelium; m3, Aspergilus fumigates; m5, Candida albicans; w1, ragweed; w6, mugwort; w7, Chrysanthemum leucanthemum; w8, dandelion; w9, plantain; w10, goosefoot; w12, goldenrod; w22, Japanese hop; wx, weed mix; g2, Bermuda grass; g3, cocksfoot; g4, meadow fescue; g5, rye-grass; g6, timothy grass; t2, alder; t3, common silver birch; t5, beech; t7, white oak; t8, elm; t10, walnut; t11, platanus; t12, willow; t13, mulberry; t15, white ash; t17, Japanese cedar; t19, acacia; t213, pine; tx, tree mix.
Table 2
Clinical Features of Enrolled Mugwort-Allergic Subjects
![ymj-57-399-i002](/upload/SynapseData/ArticleImage/0069ymj/ymj-57-399-i002.jpg)
AR, allergic rhinitis; AS, asthma; AC, allergic conjunctivitis; OAS, oral allergy syndrome; BAU, bioequivalent allergen unit; IgE, immunoglobulin E.
t1, acer; t2, alder; t3, birch; t5, beech; t7, oak; t8, elm; t10, walnut tree; t11, elder; t12, willow; t14, poplar; t16, pine; t19, acacia; t70, mulberry; g2, Bermuda grass; g3, cocksfoot; g5, rye-grass; g6, timothy grass; g8, meadow grass; g12, cultivated rye; w1, ragweed; w6, mugwort; w8, dandelion; w9, plantain; w10, goosefoot; w21, wall pellitory; w22, Japanese hop; m1, Penicillium; m2, Cladosporium; m3, Aspergilus; m5, Candia; m6, Alternaria; d1, Dermatophagodes pteronyssinus; d2, D. farinae; d72, Tyrophagus putrescentiae; e1, cat dander; e3, horse dander; e4, cow dander; e5, dog dander; e6, Guinea pig epithelium; e73, rat epithelium; e84, hamster epithelium; i6, German cockroach; i206, American cockroach; f4, wheat; f11, buckwheat.
ACKNOWLEDGEMENTS
References
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)
![crossref](/image/icon/bnr_ref_cross.gif)