Journal List > Yonsei Med J > v.44(1) > 1029557

TOOLS
Kee, Shin, Chang, Chung, Shin, Kim, Kim, and Kim: Identification of Tumor Suppressor Loci on the Long Arm of Chromosome 15 in Primary Small Cell Lung Cancer
Original Article
Yonsei Medical Journal

Yonsei Medical Journal 2003; 44(1): 65-74.

Published online: 29 March 2009

DOI: https://doi.org/10.3349/ymj.2003.44.1.65

Identification of Tumor Suppressor Loci on the Long Arm of Chromosome 15 in Primary Small Cell Lung Cancer

Hyun Jung Kee2, 6, Ju Hye Shin6, Joon Chang1, 5, Kyung Young Chung3, 5, Dong Hwan Shin4, 5, Young Sam Kim1, 5, Sung Kyu Kim1, 5, Se Kyu Kim1, 2, 5, 6

1Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.

2Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea.

3Department of Cardiovascular and Thoracic Surgery, Yonsei University College of Medicine, Seoul, Korea.

4Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.

5The Institute of Chest Diseases, Yonsei University College of Medicine, Seoul, Korea.

6Cancer Metastasis Research Center, Yonsei University College of Medicine, Seoul, Korea.

Reprint address: requests to Dr. Se Kyu Kim, Department of Internal Medicine and Cancer Metastasis Research Center, Yonsei University College of Medicine, Seoul, Korea. Tel: 82-2-361-5429, Fax: 82-2-393-6884, sekyukim@yumc.yonsei.ac.kr

Received 14 September 2002       Accepted 20 November 2002

Copyright © 2003 The Yonsei University College of Medicine

Abstract

Small cell lung cancer (SCLC) frequently shows a loss of heterozygosity (LOH) on chromosome 15q. In order to define the commonly affected region on chromosome 15q, we tested 23 primary SCLCs by microsatellite analysis.
By analyzing 43 polymorphic microsatellite markers located on chromosome 15q, we found that 14 (60.8%) of 23 tumors exhibited a LOH in at least one of the tested microsatellite markers. Two (14.3%) of the 14 tumors were found to have more than a 50% LOH on chromosome 15q. LOH was observed in five commonly deleted regions on 15q. Of those regions, LOH from D15S1012 to D15S1016 was the most frequent (47.8%). LOH was also observed in more than 20-30% of tumors at four other regions, from D15S1031 to D15S1007, from D15S643 to D15S980, from D15S979 to D15S202, and from D15S652 to D15S642. Four of the 23 tumors exhibited shifted bands in at least one of the tested microsatellite markers. Shifted bands occurred in 3.2% (29 of 914) of the loci tested.
Our data suggests the presence of at least five tumor suppressor loci on chromosome 15q in SCLC, and further that these may play an important role in SCLC tumorigenesis.

Keywords: Small cell lung cancer, LOH, microsatellite marker, chromosome 15

TOOLS
Similar articles