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Park, Park, Suh, Park, and Rah: Comparative study on motility of the cultured fetal and neonatal dermal fibroblasts in extracellular matrix
Original Article
Yonsei Medical Journal

Yonsei Medical Journal 2001; 42(6): 587-594.

Published online: 3 April 2009

DOI: https://doi.org/10.3349/ymj.2001.42.6.587

Comparative study on motility of the cultured fetal and neonatal dermal fibroblasts in extracellular matrix

Jong Chul Park1, Bong Joo Park1, Hwa Suh1, Beyoung Yun Park2, Dong Kyun Rah2

1Department of Plastic and Reconstructive Surgery and Institute for Human Tissue Restoration, Yonsei University College of Medicine, Seoul, Korea.

2Department of Medical Engineering, Institute for Human Tissue Restoration, Yonsei University, Seoul, Korea.

Reprint address: requests to Dr. Dong Kyun Rah, Department of Plastic Surgery, Institute for Human Tissue Restoration, Yonsei University College of Medicine, C.P.O. Box 8044, Seoul 120-752, Korea. Tel: 82-2-361-5690, Fax: 82-2-393-6947, dkrah@yumc.yonsei.ac.kr

Received 31 October 2001

Copyright © 2001 The Yonsei University College of Medicine

Abstract

One of the differences between fetal and adult skin healing is the ability of fetal wounds heal without contraction and scar formation.
Extracellular matrix (ECM) provides a substratum for cells adhesion, migration, and proliferation and can directly influence the form and function of cells.
As motility is essential for many important biological events, including wound healing, inflammatory response, embryonic development, and tumor metastasis, this study was designed to compare the motilities cultured dermal fetal and neonatal fibroblasts in the extracellular matrix.
The motility of cultured fetal and neonatal fibroblasts was compared using a video-microscopy system that was developed in combination with a self-designed CO2 mini-incubator. To determine migration speed, cells were viewed with a 4 × phase-contrast lens and video recorded. Images were captured using a color CCD camera and saved in 8-bit full-color mode.
We found that cultured fetal fibroblasts move faster than neonatal fibroblast on type I collagen (fetal fibroblast, 15.1 µm/hr; neonatal fibroblast, 13.7 µm/hr), and in fibronectin (fetal fibroblast, 13.2 µm/hr; neonatal fibroblast, 13.0 µm/hr) and hyaluronic acid (fetal fibroblast, 11 µm/hr; neonatal fibroblast, 9.8 µm/hr).

Keywords: Fetal fibroblast, neonatal fibroblast, migration, extracellular matrix, collagen, fibronectin, hyaluronic acid, video-microscopy system

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