To investigate the difference of dry ear wax and wet ear wax, the lipid composition of wet ear wax was analyzed and compared with that of dry ear wax. In dry ear wax, squalene, steryl esters, wax esters, triglycerides free fatty acids and cholesterol were found. Squalene, triglycerides, free fatty acids and cholesterol formed the main demonstrable fractions in wet ear wax. In addition, three unidentified spots were always present in wet ear wax. Our results indicate that wet ear wax is due to the difference of quantity and composition of ear wax lipids.