Journal List > Korean J Physiol Pharmacol > v.19(6) > 1026060

Joo, Rhie, and Jang: Enhancement of GluN2B Subunit-Containing NMDA Receptor Underlies Serotonergic Regulation of Long-Term Potentiation after Critical Period in the Rat Visual Cortex

Abstract

Serotonin [5-hydroxytryptamine (5-HT)] regulates synaptic plasticity in the visual cortex. Although the effects of 5-HT on plasticity showed huge diversity depending on the ages of animals and species, it has been unclear how 5-HT can show such diverse effects. In the rat visual cortex, 5-HT suppressed long-term potentiation (LTP) at 5 weeks but enhanced LTP at 8 weeks. We speculated that this difference may originate from differential regulation of neurotransmission by 5-HT between the age groups. Thus, we investigated the effects of 5-HT on apha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-, γ-aminobutyric acid receptor type A (GABAAR)-, and N-methyl-D-aspartic acid receptor (NMDAR)-mediated neurotransmissions and their involvement in the differential regulation of plasticity between 5 and 8 weeks. AMPAR-mediated currents were not affected by 5-HT at both 5 and 8 weeks. GABAAR-mediated currents were enhanced by 5-HT at both age groups. However, 5-HT enhanced NMDAR-mediated currents only at 8 weeks. The enhancement of NMDAR-mediated currents appeared to be mediated by the enhanced function of GluN2B subunit-containing NMDAR. The enhanced GABAAR- and NMDAR-mediated neurotransmissions were responsible for the suppression of LTP at 5 weeks and the facilitation of LTP at 8 weeks, respectively. These results indicate that the effects of 5-HT on neurotransmission change with development, and the changes may underlie the differential regulation of synaptic plasticity between different age groups. Thus, the developmental changes in 5-HT function should be carefully considered while investigating the 5-HT-mediated metaplastic control of the cortical network.

ABBREVIATIONS

ACSF

artificial cerebrospinal fluid

AMPAR

apha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor

D-AP5

D-aminopentanoate

DNQX

6,7-dinitroquinoxaline-2,3-dione

GABAAR

γ-aminobutyric acid receptor type A

LTP

long-term potentiation

NMDAR

N-methyl-D-aspartic acid receptor

OD

ocular dominance

INTRODUCTION

In the visual cortex, induction of long-term synaptic plasticity and ocular dominance (OD) plasticity decline with development [1234]. The mechanisms underlying this decline of plasticity have been extensively studied. Increase in γ-aminobutyric acid receptor type A (GABAAR)-mediated inhibition appeared to be an important determinant [56]. Changes in the subunit composition of N-methyl-D-aspartic acid receptor (NMDAR) could also be involved [7]. Another factor which could affect the decline of plasticity may be the changes in extracellular matrix [8]. All these changes are thought to participate in the decrease in synaptic plasticity. This assumption could be supported by the studies demonstrating that manipulations for enhancing plasticity in aged animals are accompanied with the changes in GABAAR-mediated inhibition, NMDAR properties, and extracellular matrix [91011]. These studies on methods to enhance plasticity in aged animals have helped to understand the underlying mechanisms in the developmental decrease in the plasticity and will provide important insights for the treatment of neurodevelopmental diseases [12].
Serotonin [5-hydroxytryptamine (5-HT)] regulates the development of neuronal network [1314] and modulates neurotransmission [15]. It also regulates the induction of long-term synaptic plasticity [1416] and OD plasticity [17]. In juvenile rats, 5-HT suppressed the induction of long-term synaptic plasticity [1418]. However, 5-HT appeared to reinstate OD plasticity in adult rats [1920]. The origin of the disparity between 5-HT regulation of long-term synaptic plasticity and OD plasticity in different age groups has been unclear. In our previous report, we also demonstrated that 5-HT suppressed long-term potentiation (LTP) in adolescent (5-week-old) rats but enhanced LTP in adult (8-week-old) rats [21]. Thus, 5-HT may have different roles in adolescent and adulthood brain. However, the mechanisms underlying the opposite regulation of LTP in the two age groups have not been addressed. Studies on the underlying mechanisms of the differential regulation of LTP may provide an insight to understand the reason for the disparity between 5-HT regulation of long-term synaptic plasticity and OD plasticity in different age groups.
Thus, in the present study, we investigated how 5-HT regulates the induction of LTP in opposite direction at different ages. To address this, we investigated the 5-HT modulation of apha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-, GABAAR-, and NMDAR-mediated synaptic transmissions and their involvement in the 5-HT regulation of LTP. Enhanced GABAAR-mediated transmission underlay the 5-HT suppression of LTP at 5 weeks. On the contrary, facilitation of LTP at 8 weeks appeared to be mediated by the enhanced function of GluN2B subunit-containing NMDAR, which could be observed only at 8 weeks. These results suggest that 5-HT could induce different metaplastic changes in the visual cortical network depending on the developmental stages.

METHODS

Slice preparation

Visual cortical slices were prepared from 5- (P35 to P41) and 8-week-old (P56 to P62) Sprague-Dawley rats of either sex (Orientbio Inc., Seoul, Korea), which were raised under the standard conditions (23±1℃, 12/12 hours light/dark cycle). Animal care and surgical procedures were conducted with the approval of the Institutional Animal Care and Use Committee of the School of Medicine at The Catholic University of Korea, and were consistent with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. The animals were sedated with chloral hydrate (400 mg/kg, i.p.) before decapitation. The brains were quickly removed to cold dissection medium consisting of (in mM) 125 NaCl, 2.5 KCl, 1 CaCl2, 2 MgSO4, 1.25 NaH2PO4, 25 NaHCO3, and 10 D-glucose, bubbled with carbogen (95% O2/5% CO2). Then coronal slices of the occipital cortex were prepared in 300 µm of thickness on a vibrotome (Campden Instruments, Leics, UK). The slices were recovered for 40 min at 37℃ in a submerging chamber with carbogenated dissection medium, and were maintained at room temperature before recording.

Recording of field excitatory postsynaptic potential (fEPSP) and LTP induction

Recording electrodes (1~2 MΩ) were pulled from borosilicate glass pipettes (1B150F-4, World Precision Instruments, Inc., Sarasota, FL, USA) using a micropipette puller (MODEL P-97, Sutter Instrument Co., Novato, CA, USA). The recording pipette was filled with artificial cerebrospinal fluid (ACSF) and the tip of the pipette was located at layer 2/3 of the visual cortical slices. ACSF consisted of (in mM) 125 NaCl, 2.5 KCl, 2 CaCl2, 1 MgSO4, 1.25 NaH2PO4, 25 NaHCO3, and 10 D-glucose. A brief rectangular current pulse (0.2 ms) was applied to evoke fEPSP with a concentric bipolar electrode located in layer 4. Stimulus intensities were adjusted to evoke fEPSP with half maximal amplitude. After more than 10 min of stable baseline recording of fEPSP, theta-burst stimulation (TBS) was applied to induce LTP. The TBS consisted of five bursts (5 Hz) of ten pulses at 100 Hz, which was applied five times (10 s interval) with the test stimulus intensity. The peak amplitude of the fEPSP, measured at 30~40 min after TBS, was compared to the baseline responses to analyze the effect of TBS.

Whole-cell voltage clamp recording

Whole-cell patch clamp recording was conducted with an EPC8 amplifier (HEKA Elektronik, Lambrecht, Germany) and pClamp 9.0 software (Axon Instruments, Foster City, CA, USA). Slices were placed in a recording chamber containing carbogenated ACSF (1.5~2 ml/min) at 32~33℃. Pyramidal neurons in layer 2/3 of the primary visual cortex were visually identified using IR-DIC video-microscopy with an upright microscope (BX51-WI fitted with a 40×/0.80 NA water immersion objective; Olympus, Tokyo, Japan). Whole-cell configuration was achieved with the recording electrodes (3~4 MΩ), and regular spiking patterns were confirmed with the square current injection. Typical access resistance was 15~20 MΩ. Data were low-pass filtered at 5 kHz and sampled at 10 kHz. K-gluconate-based pipette solution, consisting of (in mM) 130 K-gluconate, 10 KCl, 4 Mg-ATP, 10 Na2-phosphocreatine, 0.3 Na3-GTP, and 10 HEPES (pH 7.25 with KOH), was used to record AMPAR and NMDAR currents. CsCl-based pipette solution, consisting of (in mM) 145 CsCl, 4 Mg-ATP, 10 Na2-phosphocreatine, 0.3 Na3-GTP, 10 HEPES, and 3 QX-314 (pH 7.25 by CsOH), was used to record GABAAR currents. Receptor currents were evoked at -70 mV holding potential by electrical stimulation of the underlying layer 4 with a concentric bipolar tungsten electrode. AMPAR currents were recorded with normal ACSF. The AMPAR antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX, 20 µM) and the NMDAR antagonist D-aminopentanoate (D-AP5, 50 µM) were applied to record GABAAR currents. For the recording of NMDAR currents, DNQX and the GABAAR antagonist bicuculline (10 µM) were added to low Mg2+ (0.4 mM) ACSF. Amplitude of AMPAR, GABAAR, and NMDAR currents were adjusted to -500, -500, and -100 pA, respectively, for stable recording. Effects of 5-HT or 5-HT agonists on the receptor currents were assessed after 7 min of drug application. In case of AMPAR and GABAAR, the peak amplitude was compared. However, the measurement of the amplitude of NMDAR can be ambiguous due to the presence of dual peaks especially at 5 weeks. Thus, area under the current was measured to evaluate the changes of NMDAR currents. Area was calculated from the beginning of the evoked NMDAR currents to 400 ms after. The decay phase of NMDAR current was fitted by a single exponential function to assess the decay time constant, as follows:
f(t)=Aexp(-t/τ)+C.

Chemicals

The PKA inhibitor 6~22 amide (PKI) was purchased from Calbiochem (La Jolla, CA). DNQX, D-AP5, ifenprodil, bicuculline, 2-me-5-HT, NAN-190, 8-hydroxy-N,N-dipropyl-2-aminotetralin (DPAT), and 2,5-dimethoxy-4-iodoamphetamine (DOI) were purchased from Tocris (Bristol, UK). The other chemicals were purchased from Sigma (St. Louis, MO, USA).

Statistical analysis

Data are expressed as the mean±SE. Statistical comparisons were performed using paired or unpaired two-tailed Student's t-tests. The level of significance was set at p<0.05.

RESULTS

To identify the underlying mechanisms of the differential regulation of LTP induction between different age groups, we investigated the differences in 5-HT regulation of AMPAR-, GABAAR-, and NMDAR-mediated neurotransmissions between 5 and 8 weeks in the present study. Then we investigated if the differential regulation of neurotransmission underlies the differential regulation of LTP induction.

Opposite effects of 5-HT on LTP induction between 5 and 8 weeks

First, we reevaluated how 5-HT affects the induction of LTP at 5 and 8 weeks (Fig. 1). After stabilizing the baseline fEPSP recording at layer 2/3, TBS was applied to layer 4 to induce LTP. At 5 weeks, LTP induced by TBS (116.65±3.45%, n=9, p<0.01 vs. baseline) was inhibited by 10 µM of 5-HT (104.24±2.71%, n=9, p=0.157 vs. baseline, p<0.05 vs. control) (Fig. 1A). At 8 weeks, TBS did not induce LTP (98.97±2.05%, n=8, p=0.637 vs. baseline) but 5-HT reinstated LTP (117.38±3.47%, n=9, p<0.01 vs. baseline, p<0.001 vs. control) (Fig. 1B), which was in contrast to 5 weeks. Thus, 5-HT suppresses LTP at 5 weeks but facilitates LTP at 8 weeks. These results were consistent with our previous results [21]. The LTPs induced with control ACSF at 5 weeks and with 5-HT at 8 weeks were all inhibited by the NMDAR antagonist D-AP5 (98.21±1.51%, n=7, p=0.284 vs. baseline for 5 weeks; 95.86±2.29%, n=8, p=0.113 vs. baseline for 8 weeks), suggesting that these LTPs are NMDAR-dependent (Fig. 1C).

Effects of 5-HT on AMPAR-, GABAAR-, and NMDAR-mediated currents

Next, we investigated how 5-HT affects AMPAR-, GABAAR-, and NMDAR-mediated neurotransmission in layer 2/3 pyramidal neurons at 5 and 8 weeks. Receptor currents were evoked by electrical stimulation of the underlying layer 4. Cells were maintained at -70 mV holding potential. AMPAR currents were recorded with K-gluconate-based pipette solution. Amplitude of AMPAR currents were not affected by 5-HT at both 5 (511.63±8.38 pA to 505.53±10.54 pA, n=10, p=0.471) and 8 weeks (494.84±10.22 pA to 498.46±12.03 pA, n=9, p=0.631) (Fig. 2A). GABAAR currents were recorded with CsCl-based pipette solution in the presence of DNQX and D-AP5. Amplitude of GABAAR currents were enhanced by 5-HT at both 5 (493.82±9.31 pA to 584.73±22.01 pA, n=11, p<0.01) and 8 weeks (486.07±10.43 pA to 538.39±14.61 pA, n=11, p<0.01) (Fig. 2B). The increase at 8 weeks (10.7%) appeared to be smaller than at 5 weeks (18.4%), but the difference was not significant (p=0.128). NMDAR currents were recorded with K-gluconate-based pipette solution in the presence of DNQX and bicuculline. Mg2+ in ACSF was lowered to 0.4 mM. Area under the current was analyzed for NMDAR currents, instead of amplitude, because dual peaks were observed in many cases at 5 weeks as shown in Fig. 2C1. However, only single peak with fast kinetics was observed at 8 weeks. In a recent study, we demonstrated that the fast and slow peaks are mediated by GluN2A and GluN2B subunit-containing NMDAR, respectively [22]. Thus, we speculate that NMDARs containing GluN2B subunit appeared to minimally participate in the synaptic NMDAR currents at 8 weeks. In adult neocortex, major proportion of GluN2B subunit-containing NMDARs is located in extrasynaptic sites [2324]. The changes in NMDAR currents kinetics between 5 and 8 weeks in the present study (Fig. 2C) may reflect the translocation of GluN2B subunit-containing NMDARs to extrasynaptic sites. NMDAR currents were not affected by 5-HT at 5 weeks (12703.35±847.26 pA·ms to 13051.95±814.77 pA·ms, n=10, p=0.168) (Fig. 2C1). However, 5-HT enhanced NMDAR currents at 8 weeks (8764.76±435.46 pA·ms to 11285.74±746.17 pA·ms, n=12, p<0.01) (Fig. 2C2). Decay time constant of NMDAR current was also increased by 5-HT at 8 weeks (52.14±1.49 ms to 73.21±4.97 ms, p<0.01). The slower decay suggests that the increase in NMDAR currents may reflect the increase in the GluN2B subunit-containing NMDAR component, since GluN2B subunit shows slower kinetics than GluN2A subunit [25]. To address this, we tried to investigate the effects of NVP-AAM077, ifenprodil, CP-101,606, and PPDA, which are known as preferential blockers of GluN2A, GluN2B, GluN2B and GluN2C/D subunits, respectively, on 5-HT-mediated enhancement of NMDAR currents at 8 weeks. In terms of selectivity, NVP-AAM077 appeared to be not suitable, since it showed significant inhibition of both GluN2A and GluN2B subunits-mediated currents at 5 weeks even at 30 nM concentration (data not shown). Thus, we investigated the effects of ifenprodil, CP-101,606, and PPDA (Fig. 3). NMDAR currents were decreased by about 24% by ifenprodil (3 µM) (7681.12±493.52 pA·ms to 5906.62±402.55 pA·ms, n=8, p<0.001) and 5-HT had no effect in the presence of ifenprodil (to 5780.12±370.41 pA·ms, p=0.099) (Fig. 3A). CP-101,606 (3 µM) decreased NMDAR currents by about 33% (7843.71±469.04 pA·ms to 5267.14±312.01 pA·ms, n=7, p<0.001) and 5-HT had no effect in the presence of CP-101,606 (to 5126.71±350.52 pA·ms, p=0.196) (Fig. 3B). PPDA (300 nM) slightly decreased NMDAR currents by about 5% (8317.42±554.42 pA·ms to 7869.86±437.15 pA·ms, n=7, p<0.05) and 5-HT enhanced the NMDAR currents in the presence of PPDA (to 9615.42±781.84 pA·ms, p<0.01) (Fig. 3C). These results indicate that the increase in NMDAR currents by 5-HT was indeed mediated by the increase in the GluN2B subunit-containing NMDAR component. Thus, the main difference in 5-HT regulation between 5 and 8 weeks was the enhancement of the GluN2B subunit-containing NMDAR at 8 weeks.
In a previous report, we demonstrated that synaptic GABAAR currents are regulated by the activity of Ca2+/calmodulin-dependent protein kinase II (CaMKII) [26]. Enhancement of GABAAR currents by the activation of 5-HT2 receptor at 5 weeks was shown to be mediated by CaMKII [27]. We confirmed that 5-HT2 receptor and CaMKII are also responsible for the enhancement of GABAAR currents at 8 weeks in the present study (data not shown). The next question should be which molecular pathways are involved in the 5-HT modulation of NMDAR at 8 weeks. The 5-HT1A receptor antagonist NAN-190 (10 µM) had no effect on 5-HT enhancement of NMDAR currents (8966.51±414.08 pA·ms to 11151.68±640.23 pA·ms, n=10, p<0.01) (Fig. 4A1). However, the 5-HT2 receptor antagonist mesulergine (10 µM) blocked the effect of 5-HT on NMDAR (8525.64±417.94 pA·ms to 8838.51±480.85 pA·ms, n=11, p=0.081) (Fig. 4A2). In accordance with these results, the 5-HT1A receptor agonist DPAT (10 µM) had no effect on NMDAR currents (9062.85±424.67 pA·ms to 8986.9±410.06 pA·ms, n=9, p=0.567), but the 5-HT2 receptor agonist DOI (10 µM) enhanced NMDAR currents (8345.91±384.55 pA·ms to 10655.42±736.12 pA·ms, n=10, p<0.01) (Fig. 4B). The PKA, PKC, and tyrosine kinases inhibitors PKI (100 µ g/ml), chelerythrine (50 µM), and genistein (50 µM) in the pipette solution did not affect the 5-HT enhancement of NMDAR (8871.84±476.37 pA·ms to 11076.6±781.08 pA·ms, n=10, p<0.01 for PKI; 8753.06±598.61 pA·ms to 11251.94±996.65 pA·ms, n=9, p<0.01 for chelerythrine; 8053.2±448.63 pA·ms to 9983.52±561.01 pA·ms, n=10, p<0.01 for genistein) (Fig. 5A~C). However, the CaMKII inhibitor KN-93(10 µM) in the pipette solution blocked the effect of 5-HT on NMDAR (9055.6±590.92 pA·ms to 9242.59±622.2 pA·ms, n=10, p=0.365) (Fig. 5D). These results indicate that 5-HT enhancement of NMDAR currents at 8 weeks was mediated by 5-HT2 receptor and CaMKII, which is the same as the 5-HT enhancement of GABAAR.

Reversal of 5-HT effects by GABAAR antagonist and GluN2B subunit selective NMDAR antagonist

Results from previous sections demonstrated that 5-HT selectively enhanced GABAAR-mediated neurotransmission at 5 weeks. In 8 weeks, both GABAAR- and NMDAR-mediated neurotransmissions were enhanced by 5-HT via 5-HT2 receptor and CaMKII. If these 5-HT modulations of neurotransmission were responsible for the opposite regulation of LTP at 5 and 8 weeks, manipulations which can compensate the changes in neurotransmission may reverse the effects of 5-HT on LTP. To address this, we assessed the concentration of the GABAAR antagonist bicuculline and the GluN2B subunit selective NMDAR antagonist ifenprodil to reverse the increase in GABAAR- and NMDAR-mediated currents by 5-HT. Bicuculline and ifenprodil were applied 7 min after the application of 5-HT (Fig. 6). At 300 nM, bicuculline reversed the 5-HT enhancement of GABAAR-mediated currents (511.97±10.34 pA to 598.11±18.32 pA by 5-HT, to 501.05±16.79 pA by bicuculline, n=11, p=0.587 between control and bicuculline) (Fig. 6A). Ifenprodil reversed the 5-HT effects on NMDAR at 1 µM (8851.05±569.46 pA·ms to 11001.46±728.18 pA·ms by 5-HT, to 8959.67±580.92 pA·ms by ifenprodil, n=12, p=0.659 between control and ifenprodil) (Fig. 6B).
Now, we can negate the effects of 5-HT on GABAAR currents at 5 weeks and NMDAR currents at 8 weeks with 300 nM of bicuculline and 1 µM of ifenprodil, respectively. We investigated the effects of 300 nM of bicuculline and 1 µM of ifenprodil on 5-HT suppression of LTP at 5 weeks and 5-HT facilitation of LTP at 8 weeks (Fig. 7). Bicuculline reinstated the 5-HT suppressed LTP at 5 weeks (118.89±2.78%, n=9, p<0.001 vs. baseline) (Fig. 7A). Application of both bicuculline and ifenprodil reversed the effect of 5-HT on LTP at 8 weeks (103.21±2.26%, n=10, p=0.162 vs. baseline) (Fig. 7B). In addition, ifenprodil alone was enough to inhibit the 5-HT facilitated LTP at 8 weeks (101.58±2.17%, n=9, p=0.487 vs. baseline). These results collectively suggest that 5-HT suppresses LTP at 5 weeks by enhancing GABAAR-mediated neurotransmission and facilitates LTP at 8 weeks by enhancing GluN2B subunit-containing NMDAR. Thus, the opposite regulation of LTP by 5-HT originates from its differential regulation of neurotransmission between different age groups.

DISCUSSION

In the present study, we investigated the differences in 5-HT regulation of AMPAR-, GABAAR-, and NMDAR-mediated neurotransmission between the visual cortical slices of 5 and 8 week-old-rats and their roles in the regulation of LTP, to explain how 5-HT differentially regulates LTP between 5 and 8 weeks. Enhanced GABAAR currents underlay the 5-HT suppression of LTP at 5 weeks. Enhancement of GluN2B subunit-containing NMDAR, which could only be observed at 8 weeks, appeared to facilitate the induction of LTP at 8 weeks. Thus, the differential regulation of neurotransmission may underlie the differential regulation of LTP by 5-HT between the age groups.

Regulation of neurotransmission by 5-HT

Excitation-inhibition balance in cortical network can be regulated by 5-HT, since excitatory and inhibitory neurotransmissions can be differentially regulated by 5-HT [151628]. AMPAR-, GABAAR-, and NMDAR-mediated transmissions are all subject to 5-HT regulation. In hippocampus, 5-HT suppressed AMPAR-mediated component via 5-HT1A receptor [29]. 5-HT potentiated GABAAR-mediated currents in rat sacral dorsal commissural neurons via 5-HT2 receptor and PKC [30]. In the visual cortex, 5-HT enhancement of GABAAR currents was mediated by 5-HT2 receptor and CaMKII [27]. On the contrary, the activation of 5-HT2 receptor suppressed GABAAR currents in the prefrontal cortex [31]. Since GABAAR-mediated neurotransmission critically regulates the activation of NMDAR [3233], 5-HT may be able to regulate the NMDAR-dependent plasticity by modulating GABAAR currents. Furthermore, 5-HT can more directly regulate NMDAR-dependent plasticity by modulating NMDAR currents. Suppression of NMDAR component was suggested to underlie the inhibition of LTP by 5-HT in the visual cortex [34]. Increased NMDAR function by 5-HT2 receptor in basolateral amygdala appeared to facilitate LTP induction [35]. In addition, 5-HT can selectively regulate NMDAR subunits. GluN2B subunit can be selectively removed from the synapses by the activation of 5-HT1A receptor in the prefrontal cortex [36]. 5-HT2 receptor appeared to counteract the effect of 5-HT1A receptor on GluN2B subunit [37]. In this case, CaMKII was involved in the decrease in GluN2B subunit component. In our present study, CaMKII underlay the 5-HT2 receptor-mediated increase in GluN2B subunit component. These results demonstrate that 5-HT modulation of neurotransmission can be very different in various regions of the brain. This diversity may originate from the differences in the distribution of 5-HT receptor subtypes in different regions of the brain [38] and the diverse biochemical pathways associated with various 5-HT receptor subtypes [39]. Age can add more complexity as shown by the differential regulation of NMDAR currents between age groups in the present study. We speculate that the downstream biochemical pathways associated with a 5-HT receptor subtype can change or expand their connections to various molecular targets through development, since 5-HT2 receptor regulates only the GABAAR currents at 5 weeks but regulates both GABAAR and NMDAR currents at 8 weeks. GluN2B subunit-containing NMDARs are thought to exist in extrasynaptic sites at 8 weeks. We speculate that 5-HT2 receptor and CaMKII selectively translocate extrasynaptic NMDARs to synaptic sites at 8 weeks but do not affect synaptic NMDARs at 5 weeks. This assumption could possibly explain the 5-HT-induced increase in GluN2B subunit component at 8 weeks. Furthermore, the developmental changes in the expression patterns of 5-HT receptor subunits may contribute to the differential effects of 5-HT between different ages [4041]. However, this may not be a plausible explanation for the differences between 5 and 8 weeks, since the expression level of 5-HT2 receptors does not change much after early developmental period in neocortex [4142].

Metaplastic regulation of long-term synaptic plasticity by 5-HT

Metaplasticity is defined as a change in the ability to induce synaptic plasticity [43]. In the present study, 5-HT changed the inducibility of LTP at 5 and 8 weeks. Thus, the visual cortical network appears to be under the metaplastic control of 5-HT. There have been some confusing reports on 5-HT regulation of plasticity in the visual cortex. In kitten visual cortex, 5-HT is thought to be necessary for OD plasticity [1744] and long-term synaptic plasticity can be facilitated by 5-HT [45]. However, long-term synaptic plasticity was suppressed by 5-HT in the visual cortex of juvenile rats [144647]. In the more aged rats, 5-HT rather enhanced long-term synaptic plasticity [21]. The different metaplastic control by 5-HT, at different age groups in the present study, could provide important insight to understand this disparity. 5-HT may control the induction of plasticity differentially depending on the ages and species, because it can show tremendous amount of variability in the regulation of functions of various neurotransmitter receptors.
Metaplasticity has been generally thought to be induced by prior activities in the neural network [48]. The changes in the GABAAR-mediated neurotransmission can induce metaplasticity, if the activities in postsynaptic neurons were enough to send retrograde endocannabinoid-mediated signal to presynaptic GABAergic terminals [49]. Activities also regulate the expression of NMDAR subunits in the visual cortex [5051]. Activity-dependent changes in the GluN2A/GluN2B ratio appeared to be involved in the metaplasticity [95253]. In the present study, 5-HT was shown to regulate GABAAR-mediated transmission and GluN2B subunit-containing NMDAR component. These effects of 5-HT appeared to underlie the changes in the inducibility of LTP. These results indicate that neuromodulatory regulation of GABAAR- and NMDAR-mediated neurotransmissions can induce metaplasticity in the visual cortex. Thus, not only the activity-induced metaplasticity but also the neuromodulator-induced metaplasticity should be considered to fully understand the metaplastic control of neural network.

Enhancing synaptic plasticity in adult cortical network

Since synaptic plasticity is critical in the network formation and stabilization [54], the mechanisms behind the developmental decline of plasticity in the visual cortex have been of interest [15556]. Efforts to restore the plasticity in adult followed as a necessity. To this end, the roles of extracellular matrix, GABAergic inhibition, and NMDAR have been extensively studied. Extracellular matrix is an important regulator of dendritic spine dynamics and visual cortical plasticity [8]. After full development, the extracellular matrix stabilizes the neural connectivity and decreases synaptic plasticity. Thus, synaptic plasticity could be restored in adult brain when extracellular matrix was degraded [11]. GABAergic inhibition is also an important target for restoring plasticity in adult brain [57]. Manipulations which reinstate plasticity accompanied the reduction in GABAergic inhibition [102058]. The reduction of cortical inhibition itself was enough to promote plasticity in adult brain [59]. The juvenile composition of NMDAR subunits with higher GluN2B subunit-containing NMDAR appeared to be involved in the restoration of plasticity in the adult visual cortex [952]. Thus, many factors are involved in the developmental decline of plasticity and the restoration of plasticity in adult brain. Furthermore, we should also consider the regulation of plasticity by neuromodulators [606162]. One of the particularly interesting neuromodulators is 5-HT. The selective serotonin reuptake inhibitor fluoxetine has been known to restore plasticity in the adult visual cortex [19]. This effect appeared to be mediated by rather a long-term effect of 5-HT on cortical network via epigenetic remodeling of chromatin structure, which might result in increased brain-derived neurotrophic factor (BDNF) and decreased intracortical inhibition [1963]. In this case, decreased inhibition might be a critical factor for the reinstation of plasticity. In the present study, we showed that 5-HT can facilitate plasticity by enhancing GluN2B subunit-containing NMDAR component or suppress plasticity by enhancing GABAAR-mediated currents. These acute regulatory mechanisms may provide an important insight for the development of new pharmacological agents for restoring the synaptic plasticity in adult, i.e., acute and chronic effects should all be taken into account when considering 5-HT related agents.
It is now generally accepted that plasticity can be enhanced in adult nervous system by either pharmacological treatments or manipulations which shift the environmental stimulation levels [10]. Further efforts to understand the confining factors for plasticity and to facilitate plasticity in adult brain will help to make a breakthrough for the treatment of various neurodevelopmental diseases [1264].

ACKNOWLEDGEMENTS

This study was supported by the National Research Foundation of Korea (NRF-2014R1A1A1003382), which is funded by the Ministry of Science, ICT and Future Planning. The authors have no financial conflicts of interest.

ABBREVIATIONS

ACSF

artificial cerebrospinal fluid

AMPAR

apha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor

D-AP5

D-aminopentanoate

DNQX

6,7-dinitroquinoxaline-2,3-dione

GABAAR

γ-aminobutyric acid receptor type A

LTP

long-term potentiation

NMDAR

N-methyl-D-aspartic acid receptor

OD

ocular dominance

References

1. Levelt CN, Hübener M. Critical-period plasticity in the visual cortex. Annu Rev Neurosci. 2012; 35:309–330. PMID: 22462544.
crossref
2. Dudek SM, Friedlander MJ. Developmental down-regulation of LTD in cortical layer IV and its independence of modulation by inhibition. Neuron. 1996; 16:1097–1106. PMID: 8663986.
crossref
3. Kato N, Artola A, Singer W. Developmental changes in the susceptibility to long-term potentiation of neurones in rat visual cortex slices. Brain Res Dev Brain Res. 1991; 60:43–50. PMID: 1680581.
crossref
4. Kirkwood A, Lee HK, Bear MF. Co-regulation of long-term potentiation and experience-dependent synaptic plasticity in visual cortex by age and experience. Nature. 1995; 375:328–331. PMID: 7753198.
crossref
5. Sale A, Berardi N, Spolidoro M, Baroncelli L, Maffei L. GABAergic inhibition in visual cortical plasticity. Front Cell Neurosci. 2010; 4:10. PMID: 20407586.
crossref
6. Jiang B, Huang ZJ, Morales B, Kirkwood A. Maturation of GABAergic transmission and the timing of plasticity in visual cortex. Brain Res Brain Res Rev. 2005; 50:126–133. PMID: 16024085.
crossref
7. Erisir A, Harris JL. Decline of the critical period of visual plasticity is concurrent with the reduction of NR2B subunit of the synaptic NMDA receptor in layer 4. J Neurosci. 2003; 23:5208–5218. PMID: 12832545.
crossref
8. Berardi N, Pizzorusso T, Maffei L. Extracellular matrix and visual cortical plasticity: freeing the synapse. Neuron. 2004; 44:905–908. PMID: 15603733.
9. He HY, Hodos W, Quinlan EM. Visual deprivation reactivates rapid ocular dominance plasticity in adult visual cortex. J Neurosci. 2006; 26:2951–2955. PMID: 16540572.
crossref
10. Maya-Vetencourt JF, Baroncelli L, Viegi A, Tiraboschi E, Castren E, Cattaneo A, Maffei L. IGF-1 restores visual cortex plasticity in adult life by reducing local GABA levels. Neural Plast. 2012; 2012:250421. PMID: 22720172.
crossref
11. de Vivo L, Landi S, Panniello M, Baroncelli L, Chierzi S, Mariotti L, Spolidoro M, Pizzorusso T, Maffei L, Ratto GM. Extracellular matrix inhibits structural and functional plasticity of dendritic spines in the adult visual cortex. Nat Commun. 2013; 4:1484. PMID: 23403561.
crossref
12. Castrén E, Elgersma Y, Maffei L, Hagerman R. Treatment of neurodevelopmental disorders in adulthood. J Neurosci. 2012; 32:14074–14079. PMID: 23055475.
13. Sodhi MS, Sanders-Bush E. Serotonin and brain development. Int Rev Neurobiol. 2004; 59:111–174. PMID: 15006487.
crossref
14. Jang HJ, Cho KH, Park SW, Kim MJ, Yoon SH, Rhie DJ. Effects of serotonin on the induction of long-term depression in the rat visual cortex. Korean J Physiol Pharmacol. 2010; 14:337–343. PMID: 21165334.
crossref
15. Fink KB, Göthert M. 5-HT receptor regulation of neurotransmitter release. Pharmacol Rev. 2007; 59:360–417. PMID: 18160701.
crossref
16. Moreau AW, Amar M, Callebert J, Fossier P. Serotonergic modulation of LTP at excitatory and inhibitory synapses in the developing rat visual cortex. Neuroscience. 2013; 238:148–158. PMID: 23454367.
crossref
17. Gu Q, Singer W. Involvement of serotonin in developmental plasticity of kitten visual cortex. Eur J Neurosci. 1995; 7:1146–1153. PMID: 7582087.
crossref
18. Edagawa Y, Saito H, Abe K. Endogenous serotonin contributes to a developmental decrease in long-term potentiation in the rat visual cortex. J Neurosci. 2001; 21:1532–1537. PMID: 11222643.
crossref
19. Maya Vetencourt JF, Sale A, Viegi A, Baroncelli L, De Pasquale R, O'Leary OF, Castrén E, Maffei L. The antidepressant fluoxetine restores plasticity in the adult visual cortex. Science. 2008; 320:385–388. PMID: 18420937.
20. Baroncelli L, Sale A, Viegi A, Maya Vetencourt JF, De Pasquale R, Baldini S, Maffei L. Experience-dependent reactivation of ocular dominance plasticity in the adult visual cortex. Exp Neurol. 2010; 226:100–109. PMID: 20713044.
crossref
21. Park SW, Jang HJ, Cho KH, Kim MJ, Yoon SH, Rhie DJ. Developmental switch of the serotonergic role in the induction of synaptic long-term potentiation in the rat visual cortex. Korean J Physiol Pharmacol. 2012; 16:65–70. PMID: 22416222.
crossref
22. Lee C, Joo K, Kim MJ, Rhie DJ, Jang HJ. GluN2B-containing N-methyl-D-aspartate receptors compensate for the inhibitory control of synaptic plasticity during the early critical period in the rat visual cortex. J Neurosci Res. 2015; 93:1405–1412. PMID: 26013955.
crossref
23. Lopez de Armentia M, Sah P. Development and subunit composition of synaptic NMDA receptors in the amygdala: NR2B synapses in the adult central amygdala. J Neurosci. 2003; 23:6876–6883. PMID: 12890782.
crossref
24. Stocca G, Vicini S. Increased contribution of NR2A subunit to synaptic NMDA receptors in developing rat cortical neurons. J Physiol. 1998; 507:13–24. PMID: 9490809.
crossref
25. Paoletti P. Molecular basis of NMDA receptor functional diversity. Eur J Neurosci. 2011; 33:1351–1365. PMID: 21395862.
crossref
26. Joo K, Yoon SH, Rhie DJ, Jang HJ. Phasic and tonic inhibition are maintained respectively by CaMKII and PKA in the rat visual cortex. Korean J Physiol Pharmacol. 2014; 18:517–524. PMID: 25598667.
crossref
27. Jang HJ, Cho KH, Park SW, Kim MJ, Yoon SH, Rhie DJ. Layer-specific serotonergic facilitation of IPSC in layer 2/3 pyramidal neurons of the visual cortex. J Neurophysiol. 2012; 107:407–416. PMID: 22013240.
crossref
28. Moreau AW, Amar M, Le Roux N, Morel N, Fossier P. Serotoninergic fine-tuning of the excitation-inhibition balance in rat visual cortical networks. Cereb Cortex. 2010; 20:456–467. PMID: 19520765.
29. Pugliese AM, Passani MB, Corradetti R. Effect of the selective 5-HT1A receptor antagonist WAY 100635 on the inhibition of e.p.s.ps produced by 5-HT in the CA1 region of rat hippocampal slices. Br J Pharmacol. 1998; 124:93–100. PMID: 9630348.
30. Xu TL, Pang ZP, Li JS, Akaike N. 5-HT potentiation of the GABA(A) response in the rat sacral dorsal commissural neurones. Br J Pharmacol. 1998; 124:779–787. PMID: 9690871.
crossref
31. Feng J, Cai X, Zhao J, Yan Z. Serotonin receptors modulate GABA(A) receptor channels through activation of anchored protein kinase C in prefrontal cortical neurons. J Neurosci. 2001; 21:6502–6511. PMID: 11517239.
32. Luhmann HJ, Prince DA. Control of NMDA receptor-mediated activity by GABAergic mechanisms in mature and developing rat neocortex. Brain Res Dev Brain Res. 1990; 54:287–290. PMID: 1975777.
crossref
33. Kapur A, Lytton WW, Ketchum KL, Haberly LB. Regulation of the NMDA component of EPSPs by different components of postsynaptic GABAergic inhibition: computer simulation analysis in piriform cortex. J Neurophysiol. 1997; 78:2546–2559. PMID: 9356404.
crossref
34. Edagawa Y, Saito H, Abe K. Stimulation of the 5-HT1A receptor selectively suppresses NMDA receptor-mediated synaptic excitation in the rat visual cortex. Brain Res. 1999; 827:225–228. PMID: 10320714.
crossref
35. Chen A, Hough CJ, Li H. Serotonin type II receptor activation facilitates synaptic plasticity via N-methyl-D-aspartate-mediated mechanism in the rat basolateral amygdala. Neuroscience. 2003; 119:53–63. PMID: 12763068.
crossref
36. Yuen EY, Jiang Q, Chen P, Gu Z, Feng J, Yan Z. Serotonin 5-HT1A receptors regulate NMDA receptor channels through a microtubule-dependent mechanism. J Neurosci. 2005; 25:5488–5501. PMID: 15944377.
crossref
37. Yuen EY, Jiang Q, Chen P, Feng J, Yan Z. Activation of 5-HT2A/C receptors counteracts 5-HT1A regulation of n-methyl-D-aspartate receptor channels in pyramidal neurons of prefrontal cortex. J Biol Chem. 2008; 283:17194–17204. PMID: 18442977.
crossref
38. Dyck RH, Cynader MS. Autoradiographic localization of serotonin receptor subtypes in cat visual cortex: transient regional, laminar, and columnar distributions during postnatal development. J Neurosci. 1993; 13:4316–4338. PMID: 8410190.
crossref
39. Hoyer D, Hannon JP, Martin GR. Molecular, pharmacological and functional diversity of 5-HT receptors. Pharmacol Biochem Behav. 2002; 71:533–554. PMID: 11888546.
crossref
40. Roth BL, Hamblin MW, Ciaranello RD. Developmental regulation of 5-HT2 and 5-HT1c mRNA and receptor levels. Brain Res Dev Brain Res. 1991; 58:51–58. PMID: 2015654.
crossref
41. Li QH, Nakadate K, Tanaka-Nakadate S, Nakatsuka D, Cui Y, Watanabe Y. Unique expression patterns of 5-HT2A and 5-HT2C receptors in the rat brain during postnatal development: Western blot and immunohistochemical analyses. J Comp Neurol. 2004; 469:128–140. PMID: 14689478.
crossref
42. Morilak DA, Somogyi P, Lujan-Miras R, Ciaranello RD. Neurons expressing 5-HT2 receptors in the rat brain: neurochemical identification of cell types by immunocytochemistry. Neuropsychopharmacology. 1994; 11:157–166. PMID: 7865097.
crossref
43. Abraham WC, Bear MF. Metaplasticity: the plasticity of synaptic plasticity. Trends Neurosci. 1996; 19:126–130. PMID: 8658594.
crossref
44. Wang Y, Gu Q, Cynader MS. Blockade of serotonin-2C receptors by mesulergine reduces ocular dominance plasticity in kitten visual cortex. Exp Brain Res. 1997; 114:321–328. PMID: 9166921.
crossref
45. Kojic L, Gu Q, Douglas RM, Cynader MS. Serotonin facilitates synaptic plasticity in kitten visual cortex: an in vitro study. Brain Res Dev Brain Res. 1997; 101:299–304. PMID: 9263606.
crossref
46. Edagawa Y, Saito H, Abe K. Serotonin inhibits the induction of long-term potentiation in rat primary visual cortex. Prog Neuropsychopharmacol Biol Psychiatry. 1998; 22:983–997. PMID: 9789882.
crossref
47. Jang HJ, Cho KH, Joo K, Kim MJ, Rhie DJ. Differential modulation of phasic and tonic inhibition underlies serotonergic suppression of long-term potentiation in the rat visual cortex. Neuroscience. 2015; 301:351–362. PMID: 26086544.
crossref
48. Hulme SR, Jones OD, Raymond CR, Sah P, Abraham WC. Mechanisms of heterosynaptic metaplasticity. Philos Trans R Soc Lond B Biol Sci. 2013; 369:20130148. PMID: 24298150.
crossref
49. Chevaleyre V, Castillo PE. Endocannabinoid-mediated metaplasticity in the hippocampus. Neuron. 2004; 43:871–881. PMID: 15363397.
crossref
50. Yashiro K, Corlew R, Philpot BD. Visual deprivation modifies both presynaptic glutamate release and the composition of perisynaptic/extrasynaptic NMDA receptors in adult visual cortex. J Neurosci. 2005; 25:11684–11692. PMID: 16354927.
crossref
51. Philpot BD, Sekhar AK, Shouval HZ, Bear MF. Visual experience and deprivation bidirectionally modify the composition and function of NMDA receptors in visual cortex. Neuron. 2001; 29:157–169. PMID: 11182088.
crossref
52. Gagolewicz PJ, Dringenberg HC. NR2B-subunit dependent facilitation of long-term potentiation in primary visual cortex following visual discrimination training of adult rats. Eur J Neurosci. 2011; 34:1222–1229. PMID: 21895803.
crossref
53. Hager AM, Gagolewicz PJ, Rodier S, Kuo MC, Dumont ÉC, Dringenberg HC. Metaplastic up-regulation of LTP in the rat visual cortex by monocular visual training: requirement of task mastery, hemispheric specificity, and NMDA-GluN2B involvement. Neuroscience. 2015; 293:171–186. PMID: 25711939.
crossref
54. Park JM, Jung SC, Eun SY. Long-term synaptic plasticity: circuit perturbation and stabilization. Korean J Physiol Pharmacol. 2014; 18:457–460. PMID: 25598658.
crossref
55. Hensch TK. Critical period plasticity in local cortical circuits. Nat Rev Neurosci. 2005; 6:877–888. PMID: 16261181.
crossref
56. Hensch TK. Controlling the critical period. Neurosci Res. 2003; 47:17–22. PMID: 12941442.
crossref
57. Griffen TC, Maffei A. GABAergic synapses: their plasticity and role in sensory cortex. Front Cell Neurosci. 2014; 8:91. PMID: 24723851.
58. Maffei A. Enriching the environment to disinhibit the brain and improve cognition. Front Cell Neurosci. 2012; 6:53. PMID: 23162430.
crossref
59. Harauzov A, Spolidoro M, DiCristo G, De Pasquale R, Cancedda L, Pizzorusso T, Viegi A, Berardi N, Maffei L. Reducing intracortical inhibition in the adult visual cortex promotes ocular dominance plasticity. J Neurosci. 2010; 30:361–371. PMID: 20053917.
crossref
60. Gu Q. Contribution of acetylcholine to visual cortex plasticity. Neurobiol Learn Mem. 2003; 80:291–301. PMID: 14521871.
crossref
61. Kirkwood A, Rozas C, Kirkwood J, Perez F, Bear MF. Modulation of long-term synaptic depression in visual cortex by acetylcholine and norepinephrine. J Neurosci. 1999; 19:1599–1609. PMID: 10024347.
crossref
62. Seol GH, Ziburkus J, Huang S, Song L, Kim IT, Takamiya K, Huganir RL, Lee HK, Kirkwood A. Neuromodulators control the polarity of spike-timing-dependent synaptic plasticity. Neuron. 2007; 55:919–929. PMID: 17880895.
crossref
63. Maya Vetencourt JF, Tiraboschi E, Spolidoro M, Castrén E, Maffei L. Serotonin triggers a transient epigenetic mechanism that reinstates adult visual cortex plasticity in rats. Eur J Neurosci. 2011; 33:49–57. PMID: 21156002.
64. Celesia GG. Visual plasticity and its clinical applications. J Physiol Anthropol Appl Human Sci. 2005; 24:23–27.
crossref
Fig. 1

Opposite effects of 5-HT on LTP induction at 5 and 8 weeks. TBS was applied to layer 4 to induce LTP of fEPSP recorded at layer 2/3. (A) LTP induced by TBS (open circle) was inhibited by 5-HT (10 µM, closed circle) at 5 weeks. Left panel plots the amplitude of fEPSPs normalized to the baseline fEPSPs. Left upper traces show average recordings taken from representative experiments at the indicated time periods. Right panel shows individual data (symbols) and averages (thick lines) of the amplitude of fEPSPs, which were measured 30 to 40 min after TBS. ##p<0.01 vs. baseline, *p<0.05 between groups linked by lines. (B) At 8 weeks, LTP could not be induced by TBS (open diamond). However, LTP was reinstated by 5-HT (closed diamond). ##p<0.01 vs. baseline, ***p<0.001 between groups linked by lines. (C) Application of the NMDAR antagonist D-AP5 (+APV) inhibited the LTPs with control ACSF at 5 weeks (open circle) and with 5-HT at 8 weeks (closed diamond).

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Fig. 2

Effects of 5-HT on AMPAR-, GABAAR-, and NMDAR-mediated currents in layer 2/3 pyramidal neurons at 5 and 8 weeks. (A) AMPAR currents were evoked by electrical stimulation of the underlying layer 4 and were recorded with K-gluconate-based pipette solution at -70 mV holding potential. (A1) Effect of 5-HT on AMPAR currents at 5 weeks. Left panel shows traces from a representative recording. Right panel shows individual data (symbols) and averages (thick lines) of the amplitude of AMPAR currents, which were measured before and 7 min after 5-HT application. (A2) Effect of 5-HT on AMPAR currents at 8 weeks. (B) GABAAR currents were recorded with CsCl-based pipette solution at -70 mV holding potential. Amplitude of GABAAR currents were compared before and 7 min after 5-HT application. (B1) Effect of 5-HT on GABAAR currents at 5 weeks. (B2) Effect of 5-HT on GABAAR currents at 8 weeks. **p<0.01 between groups linked by lines. (C) NMDAR currents were recorded with K-gluconate-based pipette solution at -70 mV holding potential in low Mg2+ (0.4 mM) ACSF. Area under the current was compared before and 7 min after 5-HT application. (C1) Effect of 5-HT on NMDAR currents at 5 weeks. (C2) Effect of 5-HT on NMDAR currents at 8 weeks.

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Fig. 3

Importance of GluN2B subunit in the 5-HT-mediated enhancement of NMDAR currents. After 7 min application of ifenprodil, CP-101,606, and PPDA, which are known as preferential blockers of GluN2B, GluN2B and GluN2C/D subunits, respectively, 5-HT was added to the ACSF for 7 min. (A) 5-HT had no effect on NMDAR currents in the presence of ifenprodil (3 µM). Left panel shows traces of NMDAR currents at the baseline condition, after ifenprodil application, and after 5-HT application from a representative recording. Right panel shows individual data (symbols) and averages (thick lines) of the changes in the area under the NMDAR currents. (B) 5-HT had no effect on NMDAR currents in the presence of CP-101,606 (3 µM). (C) 5-HT enhanced NMDAR currents in the presence of PPDA (300 nM). *p<0.05, **p<0.01 and ***p<0.001 between groups linked by lines.

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Fig. 4

Effects of 5-HT receptor antagonists on 5-HT enhancement of NMDAR currents and 5-HT receptor agonists on NMDAR currents at 8 weeks. (A) The 5-HT1A receptor antagonist NAN-190 (A1) or the 5-HT2 receptor antagonist mesulergine (A2) were co-applied with 5-HT. Area under the current was compared before and 7 min after the application of each drug combination. Left panel shows traces from a representative recording. Right panel shows individual data (symbols) and averages (thick lines) of the changes in area under the current. **p<0.01 between groups linked by lines. (B) Effects of the 5-HT1A receptor agonist DPAT (B1) or the 5-HT2 receptor agonist DOI (B2) on NMDAR currents.

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Fig. 5

Effects of kinases inhibitors on 5-HT enhancement of NMDAR currents at 8 weeks. (A) 5-HT was applied in the presence of the PKA inhibitor PKI in pipette. Area under the current was compared before and 7 min after the application of 5-HT. Left panel shows traces from a representative recording. Right panel shows individual data (symbols) and averages (thick lines) of the changes in area under the current. **p<0.01 between groups linked by lines. (B) 5-HT was applied in the presence of the PKC inhibitor chelerythrine in pipette. (C) 5-HT was applied in the presence of the tyrosine kinases inhibitor genistein in pipette. (D) 5-HT was applied in the presence of the CaMKII inhibitor KN-93 in pipette.

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Fig. 6

Effects of bicuculline and ifenprodil on 5-HT modulation of GABAAR and NMDAR currents. The effects of 5-HT on GABAAR and NMDAR currents at 5 and 8 weeks, respectively, were assessed first, and then bicuculline and ifenprodil were applied. (A) Bicuculline (300 nM) can negate the effect of 5-HT on GABAAR currents at 5 weeks. Left panel shows traces of GABAAR currents at the baseline condition, after 5-HT application, and after bicuculline application from a representative recording. Right panel shows individual data (symbols) and averages (thick lines) of the changes in the amplitude of GABAAR currents. **p<0.01 and ***p<0.001 between groups linked by lines. (B) Ifenprodil (1 µM) can negate the effect of 5-HT on NMDAR currents at 8 weeks. Left panel shows traces of NMDAR currents at the baseline condition, after 5-HT application, and after ifenprodil application from a representative recording. Right panel shows individual data (symbols) and averages (thick lines) of the changes in the area under the NMDAR currents.

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Fig. 7

Reversal of the 5-HT regulation of LTP by bicuculline and ifenprodil. For clear comparison, data for 5-HT were reproduced from Fig. 1. (A) 5-HT suppression of LTP at 5 weeks (closed circle) was rescued by the application of bicuculline (300 nM) (open circle). (B) 5-HT enhancement of LTP at 8 weeks (closed diamond) was vanished by the application of both bicuculline and ifenprodil (1 µM, open diamond) or ifenprodil alone (grey diamond). ##p<0.01, ###p<0.001 vs. baseline, **p<0.01 between groups linked by lines.

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