Characteristics of 5-Hydroxytryptamine Receptors Involved in Contraction of Feline Ileal Longitudinal Smooth Muscle

YiYi Wang; Sun Young Park; Kyung Hoon Oh; Youngsil Min; Yun-Jeong Lee; Seok-Yong Lee; Uy Dong Sohn

doi:10.4196/kjpp.2011.15.5.267

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Wang, Park, Oh, Min, Lee, Lee, and Sohn: Characteristics of 5-Hydroxytryptamine Receptors Involved in Contraction of Feline Ileal Longitudinal Smooth Muscle

Original Article

Korean J Physiol Pharmacol 2011;15(5):267-272.

Published online: 18 February 2011

DOI: https://doi.org/10.4196/kjpp.2011.15.5.267

Characteristics of 5-Hydroxytryptamine Receptors Involved in Contraction of Feline Ileal Longitudinal Smooth Muscle

YiYi Wang¹, Sun Young Park¹, Kyung Hoon Oh¹, Youngsil Min², Yun-Jeong Lee³, Seok-Yong Lee³, Uy Dong Sohn^1,

¹Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea

²Faculty of Herb Industry, Jungwon University, Chungbuk 367-805, Korea

³Department of Pharmacology, School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea

Corresponding to: Uy Dong Sohn, Department of Pharmacology, College of Pharmacy, Chung-Ang University, 221, Heukseok-dong, Dongjak-gu, Seoul 156-756, Korea. (Tel) 82-2-820-5614, (Fax) 82-2-826-8752, (E-mail) udsohn@cau.ac.kr

Received 27 July 2011 Revised 27 September 2011 Accepted 11 October 2011

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A number of studies have demonstrated that 5-hydroxytryptamine (5-HT) can induce muscle contraction or relaxation response and enhance secretion in the gastrointestinal tract via a multiplicity of 5-HT receptor subtypes. In the present study, we investigated the pharmacological characterization of the 5-HT-induced contractile response in longitudinal smooth muscle isolated from the feline ileum. Addition of 5-HT into muscle chambers enhanced the basal tone and spontaneous activity in a concentration-dependent manner. The neurotoxin tetrodotoxin did not alter the 5-HT-induced contraction of the longitudinal muscles. Neither atropine nor guanethidine affected the contraction. The 5-HT agonists, 5-methylserotonin hydrochloride and mosapride, also evoked concentration-dependent contractions. The 5-HT-induced contraction was enhanced by the 5HT₂ receptor antagonist ketanserin and the 5-HT₃ receptor antagonist ondansetron but was inhibited by the 5-HT₁ receptor antagonist methysergide and 5-HT₄ receptor antagonist GR113808. These results indicate that 5-HT₁ and 5-HT₄ receptors may mediate the contraction of the 5-HT-induced response and 5-HT₂ and 5-HT₃ receptors may mediate 5-HT-induced relaxation in feline ileal longitudinal smooth muscles.

Keywords: Ileum, Longitudinal smooth muscle, 5-HT, Contraction, Relaxation

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Fig. 1.

Representative tracing showing the effect of 5-HT on the spontaneous contraction of feline longitudinal smooth muscle. 5-HT was added cumulatively (10^–10∼10^–6 M) with 5-min contact time for each concentration.

Fig. 2.

Effect of atropine or guanethidine on 5-HT-induced contraction. Concentration-response curves to 5-HT in the absence (❍) and presence of 0.1 μM atropine (), (A) or 1 μM guanethidine (▪), (B). Preparations were incubated with atropine or guanethidine for 10 min before and during 5-HT treatment. Each point represents the mean±S.E.M. (n=6).

Fig. 3.

Effect of TTX on 5-HT-induced contraction. Concentration-response curves to 5-HT in the absence (❍) and presence of 0.1 μM () and 1 μM (▪) TTX. Preparations were incubated with TTX for 10 min before and during 5-HT treatment. Each point represents the mean±S.E.M. (n=5).

Fig. 4.

Concentration-response curves for 5-HT- (❍, n=11), 5-MOT-(, n=8) and mosapride- (▪, n=10) induced contraction. Each point represents the mean±S.E.M.

Fig. 5.

Inhibitory effect of 5-HT receptor antagonists on 5-HT-induced contraction. The preparation was incubated with (A) methysergide (1 μM, n=5), (B) ketanserin (0.1 μM, n=8), (C) ondansetron (1 μM, n=8) or (D) GR113808 (10 nM, n=5) for 30 min before and during 5-HT treatment. Absence (❍) or presence () of antagonist. Each point represents the mean±S.E.M. ^∗p<0.05 and ^∗∗p<0.01 compared to the same concentration of 5-HT-induced contraction alone.

Fig. 6.

Major roles of 5-HT receptors in isolated feline ileum longitudinal smooth muscle. 5-HT can stimulate 5-HT₁ or 5-HT₄ receptors resulting in contraction of smooth muscle. 5-HT can also stimulate 5-HT₂ or 5-HT₃ receptors resulting in relaxation of smooth muscle.

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