Journal List > Korean J Physiol Pharmacol > v.14(5) > 1025691

TOOLS
Park, Sim, Lim, Kim, Lee, and Suh: Antinociception Effect and Mechanisms of Campanula Punctata Extract in the Mouse
Original Article

Korean J Physiol Pharmacol 2010;14(5):285-289.

Published online: 18 February 2010

DOI: https://doi.org/10.4196/kjpp.2010.14.5.285

Antinociception Effect and Mechanisms of Campanula Punctata Extract in the Mouse

Soo-Hyun Park1, 2, †, Yun-Beom Sim1, 2, †, Soon-Sung Lim2, Jin-Kyu Kim2, Jin-Koo Lee2, Hong-Won Suh1, 2, ∗

1Departments of Pharmacology, College of Medicine, Hallym University, Chuncheon 200-702, Korea

2Institute of Natural Medicine, College of Medicine, Hallym University, Chuncheon 200-702, Korea

∗Corresponding to: Hong-Won Suh, Department of Pharmacology, College of Medicine, Hallym University, 1, Okchun-dong, Chuncheon 200-702, Korea. (Tel) 82-33-248-2614, (Fax) 82-33-248-2612, (E-mail) hwsuh@hallym.ac.kr

† Equally contributed to first author.

Received 5 August 2010       Revised 10 September 2010       Accepted 14 September 2010

Copyright © 2010 Korean J Physiol Pharmacol

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the present study, the antinociceptive profiles of Campanula punctata extract were examined in ICR mice. The Campanula punctata contain a large dose of saponin. Campanula punctata extract administered orally (200 mg/kg) showed an antinociceptive effect as measured by the tail-flick and hot-plate tests. In addition, Campanula punctata extract attenuated the writhing numbers in the acetic acid-induced writhing test. Furthermore, the cumulative nociceptive response time for intrathecal (i.t.) injection of substance P (0.7 μg) was diminished by Campanula punctata extract. Intraperitoneal (i.p.) pretreatment with yohimbine (α2-adrenergic receptor antagonist) attenuated antinociceptive effect induced by Campanula punctata extract in the writhing test. However, naloxone (opioid receptor antagonist) or methysergide (5-HT serotonergic receptor antagonist) did not affect antinociception induced by Campanula punctata extract in the writhing test. Our results suggest that Campanula punctata extract shows an antinociceptive property in various pain models. Furthermore, this anti-nociceptive effect of Campanula punctata extract may be mediated by α2-adrenergic receptor, but not opioidergic and serotonergic receptors.

Keywords: Campanula punctata, Anti-nociception, Inflammatory pain, α2 adrenoceptor

References

1. Kathleen N, Brenzel ED. Sunset Western Garden Book. 6th ed.Sunset Publishing Corporation;1995. p. 606–607.
2. Shin CY, Lee WJ, Lee EB, Choi EY, Ko KH. Platycodin D and D3 increase airway mucin release in vivo and in vitro in rats and hamsters. Planta Med. 2002; 68:221–225.
crossref
3. Hylden JL, Wilcox GL. Intrathecal morphine in mice: a new technique. Eur J Pharmacol. 1980; 67:313–316.
crossref
4. Hylden JL, Wilcox GL. Intrathecal substance P elicits a caudally-directed biting and scratching behavior in mice. Brain Res. 1981; 217:212–215.
crossref
5. D'Amour FE, Smith DL. A method for determining loss of pain sensation. J Pharmacol Exp Ther. 1941; 72:74–79.
6. Eddy NB, Leimbach D. Synthetic analgesics. II. Dithienylbutenyl- and dithienylbutylamines. J Pharmacol Exp Ther. 1953; 107:385–393.
7. Koster R, Anderson M, Beer EJ. Acetic acid for analgesic screening. Federal Proceeding. 1959; 18:412.
8. Choi SS, Han KJ, Lee JK, Lee HK, Han EJ, Kim DH, Suh HW. Antinociceptive mechanisms of orally administered decursinol in the mouse. Life Sci. 2003; 73:471–485.
crossref
9. Park SH, Sim YB, Choi SM, Seo YJ, Kwon MS, Lee JK, Suh HW. Antinociceptive Profiles and Mechanisms of Orally Administered Vanillin in the Mice. Arch Pharm Res. 2009; 32:1643–1649.
crossref
10. Suh HW, Song DK, Son KH, Wie MB, Lee KH, Jung KY, Do JC, Kim YH. Antinociceptive mechanisms of dipsacus saponin C administered intracerebroventricularly in the mouse. General Pharmacology. 1996; 27:1167–1172.
crossref
11. Suh HW, Song DK, Kim YH. Differntial effects of adenosine receptor antagonist injected intrathecally on antinociception induced by morphine and beta-endorphin administered intra-cerebroventricularly in the mouse. Neuropeptides. 1997; 31:339–344.
12. Suh HW, Chung KM, Kim YH, Huh SO, Song DK. Effect of histamine receptor antagonists injected intrathecally on antinociception induced by opioids administered intracerebroventricularly in the mouse. Neuropeptides. 1999; 33:121–129.
13. Chapman CR, Casey KL, Dubner R, Foley KM, Gracely RH, Reading AE. Pain measurement: an overview. Pain. 1985; 22:1–31.
crossref
14. Grumbach L. The prediction of analgesic activity in man by animal testing. Knighton RS, Dumke PR, editors. Pain. Boston: Little Brown and Co;1966. p. 163–182.
15. Vyklicky L. The techniques for the study of pain in animals. Bonica JJ, Liebeskind JC, Albe-Fessard DG, editors. Adv Pain Res Ther. Vol. 3. New York: Raven.;1979. p. 727–745.
16. Cumberbatch MJ, Herrero JH, Headley PM. Exposure of rat spinal neurons to NMDA, AMPA and kainate produces only short-term enhancements of responses to noxious and non-noxious stimuli. Neuroscience Letters. 1994; 181:98–102.
17. Schmauss C, Yaksh TL. In vivo studies on spinal opiate receptor systems mediating antinociception. II. Pharmacological profiles suggesting a differential association of mu, delta and kappa receptors with visceral chemical and cutaneous thermal stimuli in the rat. J Pharmacol Exp Ther. 1984; 228:1–12.
18. Yaksh TL. Direct evidence that spinal serotonin and noradrenaline terminals mediate the spinal antinpciceptive effects of morphine in the periaqueductal gray. Brain Res. 1979; 160:180–185.
19. Yaksh TL. Multiple opioid receptor systems in brain and spinal cord: Part I. Eur J Anaesthesiol. 1984; 1:171–199.
20. Jensen TS, Yaksh TL. Spinal monoamine and opiate systems partly mediate the antinociceptive effects produced by glutamate at brainstem sites. Brain Research. 1984; 321:287–297.
crossref
21. Wigdor S, Wilcox GL. Central and systemic morphine-induced antinociception in mice: contribution of descending serotonergic and noradrenergic pathways. Journal of pharmacology and Experimental Therapeutics. 1987; 242:90–95.

Fig. 1.
The antinociceptive effect of Campanula punctata extract administered orally in the tail-flick and hot-plate tests. Mice were administered orally with either vehicle or 200 mg/kg of Campanula punctata extract and the tail-flick (A) or hot-plate (B) response was measured at 30 min after treatment. The vertical bars denote the standard error of the mean. The number of animal used for each group was 8∼10 (∗p<0.05, ∗∗p<0.01, compared to the vehicle-treated control group of mice).
kjpp-14-285f1.tif
Fig. 2.
Effect of Campanula punctata extract on the nociceptive response induced by various pain models. Campanula punctata extract (200 mg/kg) was administered orally and then, 0.25 ml of 1% acetic acid solution was injected intraperitoneally 30 min after treatment. The number of writhing was counted for 30 min following acetic acid injection (A). Campanula punctata extract (200 mg/kg) was administered orally for 30 min prior to the substance P (0.7 μg per 5 μl) injection intrathecally (B). The cumulative response time of licking, scratching and biting episodes was measured for 30 min. The vertical bars indicate the standard error of the mean. The number of animal used for each group was 8∼10 (∗∗∗p < 0.001, compared with control group).
kjpp-14-285f2.tif
Fig. 3.
Effect of naloxone (A), methysergide (B) and yohimbine (C) injected intraperitoneally (i.p.) on inhibition of the writhing response induced by Campanula punctata extract administered orally. Naloxone, methysergide, or yohimbine (5 mg/kg) was pretreated intraperitoneally for 10 min, before oral administration of vehicle or Campanula punctata extract (200 mg/kg). Campanula punctata extract or vehicle was administered orally and then, 0.25 ml of 1% acetic acid solution was injected i.p. 30 min after treatment. The number of writhing was counted for 30 min following acetic acid injection. The vertical bars denote the standard error of the mean. The number of animal used for each group was 8∼10 (∗∗∗ p<0.001, compared with control group and only antagonist group).
kjpp-14-285f3.tif
TOOLS
Similar articles