Ascorbic Acid Enhances Nitric Oxide Production in Trabecular Meshwork Cells

Jae Woo Kim

doi:10.3341/kjo.2005.19.3.227

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Kim: Ascorbic Acid Enhances Nitric Oxide Production in Trabecular Meshwork Cells

Original Article

Korean J Ophthalmol 2005;19(3):227-232.

Published online: 21 December 2005

DOI: https://doi.org/10.3341/kjo.2005.19.3.227

Ascorbic Acid Enhances Nitric Oxide Production in Trabecular Meshwork Cells

Jae Woo Kim, MD

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea

Reprint requests to Jae Woo Kim, MD. Department of Ophthalmology, Catholic University of Daegu College of Medicine, #3056-6 Daemyeung-4-dong, Nam-gu, Daegu 705-718, Korea. Tel: 82-53-650-4728, Fax: 82-53-627-0133, E-mail: jwkim@cu.ac.kr

Received 18 March 2005 Accepted 4 August 2005

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

This study investigated the role of ascorbic acid on the production of nitric oxide (NO) in the trabecular meshwork (TM) cells.

Methods

After primarily cultured human TM cells were exposed to 1, 10, and 100 µM of L-ascorbic acid (LAA), with or without co-administration of 1 mM sodium nitroprusside or 100 µM hydrogen peroxide for 48 hr, cellular survival and NO production were measured with MTT and Griess assay, respectively.

Results

LAA significantly potentiated NO production in a dose-dependent manner (p<0.05) without affecting cell viability. LAA increased cell viability after hydrogen peroxide-induced oxidative stress in a dose-dependent manner. LAA enhanced NO production in TM cells and showed a cytoprotective effect against hydrogen peroxide-induced oxidative stress.

Conclusions

LAA might be involved in the regulation of trabecular outflow by enhancing NO production in TM cells.

Keywords: Ascorbic acid, Hydrogen peroxide, Nitric oxide, Trabecular meshwork cell

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Fig. 1.

Effect Of L-ascorbic acid on the viability of cultured trabecular meshwork cells. L—ascorbic acid does not affect significantly on the Viability of trabecular meshwork cells up to 100 uM

Fig. 2.

Effect of 3 mM sodium nitroprusside and 1 mM hydrogen peroxide on the survival of cultured trabecular meshwork cells (∗p<0.05).

Fig. 3.

Effect of L—ascorbic acid on the production of nitrite in cultured trabecular meshwork cells. L—ascorbic acid potentiate nitrate production significantly in a dose-dependent manner and L—NAME inhibited nitrite production significantly (∗p<0.05).

Fig. 4.

Effect of L—ascorbic acid on the production of nitric oxide exposed to 3 mM sodium nitroprusside and 1 mM hydrogen peroxide in Cultured trabecular meshwork cells (∗p<0.05).

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