Cat scratch disease (CSD) is a worldwide zoonosis caused by Bartonella henselae or possibly by Bartonella clarridgeiae (1-3). It is characterized usually a self-limiting regional lymphadenopathy, associated with a cat scratch or bite. Originally considered rare, it is now recognized as a common cause of lymphadenopathy in children and young adults (2). Classic systemic disease includes a cutaneous inoculation by a scratch or bite followed by a regional lymphadenopathy after a variable period, ranging from 1 to 8 weeks. The number of pet cats is increasing in developed countries including Korea. According to the increase in number of pet cats, zoonosis like CSD has risen as a health problem in human society. In the past most cases of CSD were diagnosed by clinical manifestations and intradermal reaction with specimens taken from patients before isolation of the causative organisms. Due to the difficulty of isolation of B. henselae from CSD patient, the diagnosis is usually based on serologic data and clinical history when informative. Recently polymerase chain reaction (PCR) is used as a confirmative method with biopsy or aspiration specimen of lymph nodes from CSD patients (4-6). In Korea, there is no reported case of CSD confirmed by PCR. This report deals with a case of CSD confirmed by PCR assay using different sets of primers.

A 25-yr-old previously healthy woman visited Sanggyepaik Hospital with high fever over 7 days and painful mass in the left neck. In spite of medication of oral antibiotics at a private clinic, her symptoms were not improved. She had been admitted to our hospital on 7 May 2004. She had been keeping a dog for 4 months before admission but had no history of contact with a cat.

On admission there were multiple palpable mass in the left neck area. The masses were 2 cm and 1.5 cm in diameter, and were tender. On physical examination liver and spleen was not palpable. There was no skin lesion or scratched wound in the face, extremity and trunk.

She had a white cell count of 3,490×10⁹/L (neutrophil, 87%; lymphocyte, 8.6%; monocyte, 3.2%), with platelets 100×10⁹/L, a hemoglobin of 12.1 g/dL. Blood chemistry revealed: AST 71 IU/L, ALT 62 IU/L, total bilirubin 0.3 mg/dL, BUN 8 mg/dL, creatinine 0.7 mg/dL. Laboratory findings showed elevated CRP, but ESR was 3 mm/hr. ANA and anti-dS DNA was negative. The computed tomography of the patient's neck showed multiple variable-sized lymph nodes (maximum 16×10 mm). The serum sample from the patient was tested for B. henselae antibodies by using a commercial immunofluorescent assay (Bartonella IFA IgG; Focus technologies, Cypress, CA, U.S.A.). The IgG titer was 1:64 positive. Aspiration cytology of lymph node of left neck revealed reactive hyperplasia.

The patient started receiving clindamycin intravenously for 6 days after lymph node aspiration. The fever and pain in the left neck area persisted during the treatment. Under the impression of reactive lymphadenitis she had been discharged without medication. During the outpatient clinic follow up her symptoms improved gradually without medication and completely recovered one month later. She had remained asymptomatic for 3 months.

CSD, caused by Bartonella henselae, is a worldwide zoonosis associated with a variety of clinical manifestations. Typically, a nontender papule develops in the scratch line, three to 10 days after exposure, healing without scarring in two or three weeks. Regional lymphadenopathy follows in more than 80% of cases, but resolves usually within two to three months (7). Atypical CSD are reported up to 25% of cases (8). The manifestations of atypical CSD are fever of unknown origin, neuroretinitis, encephalopathy, hepatosplenic granuloma, juvenile rheumatoid arthritis. Nowadays, B. henselae infection is regarded as a common cause among patients with fever of unknown origin (9). Atypical presentations are considered as manifestations of Bartonella infection rather than CSD. The epidemiological and clinical characteristics of CSD have been well delineated in countries other than Korea (2, 8). Epidemiological and clinical study of CSD in Korea is rare (10). Domestic cats or dogs are the reservoirs for B. henselae and it is transmitted through scratches or bites (7, 11, 12). However, no history of animal contact can be elicited in small percentage of CSD patient.

Cat scratch disease is usually a self-limiting disease and does not require therapy. But, some patients with multi-system involvement may benefit from antibiotics treatment, so it is necessary to identify the organism rapidly by clinical laboratory assay. The diagnosis of CSD is made currently on the basis of clinical criteria in addition to a recent history of cat or dog exposure, a scratch or a flea bite plus bacteria culture, histologic examination of tissue biopsies and serologic test. Although serologic analysis by immunofluorescence or enzyme linked immnuosorbent assay is a useful tool for the diagnosis of B. henselae infection, the specificity of serological assay has been questioned due to the cross reactivity between B. henselae and other species (13). Also antigenic variability within the species could partly explain inconsistent results in the serological diagnosis of CSD.

PCR assays appear to be very useful in confirming clinically suspected CSD and have advantage of rapid diagnosis with the reliability since it is independent on the patient's humoral response. Recent studies relied on PCR amplification to improve diagnosis of CSD (4-6, 14). Earlier assays targeted amplification of 16S rRNA gene which is present in all bacteria with species polymorphism (14). PCR assay using the amplification of a portion of the citrate synthase gene (gltA) followed by TaqI restriction digest of the products is a sensitive tool for the detection of B. henselae DNA in tissue biopsy specimens and pus aspirates from lymph nodes of patients with CSD, but require large amounts of clinical material. The pap 31 gene encodes a major protein associated with a phage from B. henselae and allowed the classification of its strains into two clusters, B. henselae Houston-1 and B. henselae Marseille (6). Avidor et al. (14) demonstrated that PCR diagnosis of CSD from fine needle aspiration and primary lesion specimens can be minimally invasive and highly accurate, so precluding the necessity for excision biopsy. In this case, antibody titer to B. henselae was positive (1:64) and corresponding sequence to major genogroup B. henselae Houston was detected by PCR analysis of lymph node tissue by fine needle aspiration. PCR offers a rapid and specific means to detect the organism directly from clinical specimens in CSD patients. And it is more sensitive than isolation when performed on suitable clinical samples such as fresh or frozen lymph node tissues.

In conclusion, when presented with lymphadenopathy, physicians should inquire about recent cat, dog, or pets contact and/or animal scratches considering the possibility of CSD.