Gene Expression in the Capsule Tissue of the Frozen Shoulder

Jung-Man Kim; Yang-Soo Kim; Jong-Hun Ji; Jae-Young Lee; Hyun-Ho Yoo; Youn-Kyoung Lee

doi:10.4055/jkoa.2010.45.1.65

Journal List > J Korean Orthop Assoc > v.45(1) > 1013044

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Kim, Kim, Ji, Lee, Yoo, and Lee: Gene Expression in the Capsule Tissue of the Frozen Shoulder

Original Article

Journal of the Korean Orthopaedic Association

Journal of the Korean Orthopaedic Association 2010; 45(1): 65-72.

Published online: 28 February 2010

DOI: https://doi.org/10.4055/jkoa.2010.45.1.65

Gene Expression in the Capsule Tissue of the Frozen Shoulder

Jung-Man Kim, M.D., Yang-Soo Kim, M.D., Jong-Hun Ji, M.D.^*, Jae-Young Lee, M.D., Hyun-Ho Yoo, M.D., Youn-Kyoung Lee, M.S.

Department of Orthopaedic Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea College of Medicine, Seoul, Korea.

^*Department of Orthopaedic Surgery, Daejeon St. Mary's Hospital, The Catholic University of Korea College of Medicine, Daejeon, Korea.

Correspondence to: Yang-Soo Kim, M.D. Department of Orthopaedic Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea College of Medicine, 505, Banpo-dong, Seocho-gu, Seoul 137-040, Korea. TEL: +82-2-2258-6117, FAX: +82-2-535-9834, kysoos@catholic.ac.kr

Received 14 June 2009 Accepted 10 December 2009

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the overall expression of extracellular matrix (ECM) and adhesion molecule genes using a gene array technique in the joint capsule of a frozen shoulder.

Materials and Methods

Tissues from 20 human shoulder joint capsules were harvested intraoperatively from patients (15 primary frozen shoulders, 5 controls) in our hospital. The RNA was isolated from the capsule tissue and the gene expression of ECM and adhesion molecules was analyzed using an oligo-array technique.

Results

The expression of several genes of the ECM and cell adhesion molecules was significantly higher in the capsule tissue from patients with a frozen shoulder than the controls. The gene expression of the collagen V α1/α3, VI α2/α3, VIII α1/α2, XV α1, XVIII α1 and ECM proteins including CD 44, connective tissue growth factor (CTGF), matrix metalloproteinase (MMP)-9/14, osteonectin, veriscan, hyaluronan synthase (HAS)-1, extra-cellular matrix (ECM)-1, secreted phosphoprotein (SSP)-1, tenascin C (TNC), thrombospondin 2/4 was two times higher in the frozen shoulder than the control. Several cell adhesion molecules genes including catenin α1, seletin p, integrin α3, β2, β4, β5 and laminin α4, α5 were also two times higher in the in the patients with a frozen shoulder than the control.

Conclusion

The gene expression of several ECM proteins and adhesion molecules is significantly higher in patients with frozen shoulder.

Keywords: frozen shoulder, oligo-array, extracellular matrix, adhesion molecule

Figures and Tables

Figure 1

Representative examples of microarray blots of human ECM and adhesion molecules from capsule tissue of the frozen shoulder patient and control. (A) Each spot on the microarray membrane represents the expression of an individual gene. For example, the red arrow indicate the MMP-9 gene on the membrane, which is expressed as dark black dot on the frozen shoulder compared with gray dot on the control shoulder. (B) The map of 128 genes on the membrane of GEArray® Series for Human Extracellular Matrix & Adhesion Molecules (Bioscience Corporation, Fredrick, MD). β-2-microglobulin (B2M) and β-actin (ACTB) gene are considered as a control genes, which should have the same expression on both membranes.

Table 1

Collagens List of Up-regulated Genes in Capsular Specimen from the Patients with Frozen Shoulder (p<0.05)

Table 2

Cell Adhesion Molecules List of Up-regulated Genes in Capsular Specimen from the Patients with Frozen Shoulder (p<0.05)

Table 3

Extracellular Matrix Proteins List of Up-regulated Genes in Capsular Specimen from the Patients with Frozen Shoulder (p<0.05)

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