Journal List > J Korean Orthop Assoc > v.37(2) > 1011986

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Shin, Kang, Kim, Kang, Hahn, Kim, Han, and Lee: Effect of Growth Factors on Type I Collagen Synthesis in Cultured Rabbit's Deep Flexor Tendon Cell
Original Article

Journal of the Korean Orthopaedic Association 2002; 37(2): 288-294.

Published online: 4 January 2018

DOI: https://doi.org/10.4055/jkoa.2002.37.2.288

Effect of Growth Factors on Type I Collagen Synthesis in Cultured Rabbit's Deep Flexor Tendon Cell

Dong Eun Shin, Ho Jung Kang, Hyun Woo Kim, Eung Shick Kang, Soo Bong Hahn, Sung Jae Kim, Chang Dong Han, Woo Suk Lee

Department of Orthopaedic Surgery, Pochon CHA Medical College, Korea.

Department of Orthopaedic Surgery, Yonsei University College of Medicine, Korea.

Department of Orthopaedic Surgery, Konyang University College of Medicine, Seoul, Korea.

os3410@yumc.yonsei.ac.kr

Copyright © 2002 The Korean Orthopaedic Association

Abstract

Purpose

This study focuses on the change of the expression of type-I collagen mRNA and extent of collagen synthesis caused by four kinds of growth factos (TGF-b, IGF-I, EGF, PDGF) and their combinations to elucidate the effects of growth factors on rabbit's flexor tendon.

Materials and Methods

Cells were isolated from rabbit's flexor tendons and cultured with DMEM containing 0.5% fetal bovine serum. Growth factors were then given either independently or in combination. On the 5th, 10th, and 15th day, the cells were collected and the cell proliferation rate was analyzed. The expression of type-I collagen mRNA was analyzed by Northern blot.

Results

The expression of type-I collagen mRNA increased significantly in the IGF, PDGF, PDGF/EGF, and PDGF/IGF groups (p<0.05). Western blot analysis showed that the extent of type-I collagen synthesis in culture media was similar to the type-I collagen mRNA expression.

Conclusion

Based on this study, we conclude that PDGF and IGF individually, and the combinations PDGF/IGF and PDGF/EGF were most effective at aiding the healing process in rabbit's flexor tendon.

Keywords: Tendon cell culture, Growth factor, Type-I collagen, Rabbit flexor tendon

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