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Suh, Chun, Yong, Lee, Jeong, Yang, and Kim: A Report of Cat Scratch Disease in Korea Confirmed by PCR Amplification of the 16S-23S rRNA Intergenic Region of Bartonella henselae
Brief Communication

Korean J Leg Med 2010;30(1):34-37.

Published online: 14 January 2010

DOI: https://doi.org/10.3343/kjlm.2010.30.1.34

A Report of Cat Scratch Disease in Korea Confirmed by PCR Amplification of the 16S-23S rRNA Intergenic Region of Bartonella henselae

Borum Suh, M.D.1, Jin-Kyoung Chun, M.D.2, Dongeun Yong, M.D.1, 4, Yang Soon Lee, M.D.1, 4, Seok Hoon Jeong, M.D.1, 4, Woo Ick Yang, M.D.3, Dong Soo Kim, M.D.2

1Departments of Laboratory Medicine, Seoul, Korea

2Departments of Pediatrics, and Pathology, Seoul, Korea

3Departments of Research Institute of Bacterial Resistance, Seoul, Korea

4Yonsei University College of Medicine, Seoul, Korea

Corresponding author: Dong Eun Yong, M.D. Department of Laboratory Medicine, Yonsei University College of Medicine, 250 Seongsan-ro, Seodaemun-gu, Seoul 120-752, Korea Tel: +82-2-2228-2445 Fax: +82-2-313-0956 E-mail: deyong@yuhs.ac

      Revised 26 September 200923 November 2009       Accepted 9 December 2009

Copyright © 2010 Korean Society for Legal Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We report a case of cat scratch disease in an 8-yr-old girl who presented with fever and enlargement of both axillary lymph nodes. Both aerobic and anaerobic cultures of the lymph node aspirate were negative for microbial growth. Gram staining and Warthin-Starry silver staining did not reveal any organism. Purified DNA from the PCR-amplicon of the 16S-23S rRNA intergenic region was sequenced and showed 99.7% identity with the corresponding sequence of Bartonella henselae strain Houston-1. Our findings suggest that the internal transcribed spacer is a reliable region for PCR identification of Bartonella species. In patients with lymphadenitis, a history of contact with cats or dogs necessitates the use of diagnostic approaches that employ not only the conventional staining and culture but also molecular methods to detect B. henselae. (Korean J Lab Med 2010;30:34-7)

Keywords: Bartonella henselae, Cat scratch disease, Polymerase chain reaction

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