Journal List > Korean J Lab Med > v.30(3) > 1011645

Hwang, Kim, Yoon, Cha, Kim, Yong, Chang, Jeong, Uh, and Lee: First Report of Brain Abscess Associated with Pseudozyma species in a Patient with Astrocytoma

Abstract

A yeast-like strain was isolated from the brain abscess of a patient diagnosed with astrocytoma. Morphological and molecular analysis on D1/D2 domain in the 26S rRNA gene and internal transcript spacer region of the strain revealed that the strain belonged to the genus Pseudozyma. To the best of our knowledge, this is the first report on the isolation of a Pseudozyma strain from brain abscess.

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Fig. 1.
(A) Colony morphology after 3 days of incubation on Sabouraud's dextrose agar at 30°C and (B) gram staining showing fusiform yeast with budding (Gram stain,×1,000). (C) Slide culture on Sabouroud's dextrose agar after 3 days of incubation at 30°C in ambient air showing elongated spindle-shaped blastoconidia. Scale bar=10 μm.
kjlm-30-284f1.tif
Fig. 2.
Molecular phylogenetic tree of strain YCM09/6/F44 and other related reference species derived from the sequence of D1/D2 domain of 26S rRNA. The bar indicates genetic distance.
kjlm-30-284f2.tif
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