Journal List > Korean J Lab Med > v.30(2) > 1011618

TOOLS
Yang, Kim, Choi, and Jang: Development and Evaluation of the Quick Anaero-system-A New Disposable Anaerobic Culture System
Original Article

Korean J Leg Med 2010;30(2):133-137.

Published online: 14 January 2010

DOI: https://doi.org/10.3343/kjlm.2010.30.2.133

Development and Evaluation of the Quick Anaero-system-A New Disposable Anaerobic Culture System

Nam-Woong Yang, M.D.1, Jin-Man Kim, M.D.2, Gwang-Ju Choi, M.D.2, Sook-Jin Jang, M.D.3

1Departments of Microbiology, Chosun University Medical School, Gwangju, Korea

2Ophthalmology, Chosun University Medical School, Gwangju, Korea

3Laboratory Medicine, Chosun University Medical School, Gwangju, Korea

Corresponding author: Sook Jin Jang, M.D. Department of Laboratory Medicine, Research Center for Resistant Cells, Chosun University Medical School, 588 Seoseok-dong, Dong-gu, Gwangju 501-717, Korea Tel: +82-62-220-3259 Fax: +82-62-232-2063 E-mail: sjbjang@chosun.ac.kr

∗ This work was financially supported by Chosun University, 2002.

      Revised 30 July 200929 January 2010       Accepted 10 February 2010

Copyright © 2010 Korean Society for Legal Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background:

We developed a new disposable anaerobic culture system, namely, the Quick anaero-system, for easy culturing of obligate anaerobes.

Methods:

Our system consists of 3 components: 1) new disposable anaerobic gas pack, 2) disposable culture-envelope and sealer, and 3) reusable stainless plate rack with mesh containing 10 g of palladium catalyst pellets. To evaluate the efficiency of our system, we used 12 anaerobic bacteria. We prepared 2 sets of ten-fold serial dilutions of the 12 anaerobes, and inoculated these samples on Luria-Bertani (LB) broth and LB blood agar plate (LB-BAP) (BD Diagnostic Systems, USA). Each set was incubated in the Quick anaero-system (DAS Tech, Korea) and BBL GasPak jar with BD GasPak EZ Anaerobe Container System (BD Diagnostic Systems) at 35-37°C for 48 hr. The minimal inoculum size showing visible growth of 12 anaerobes when incubated in both the systems was compared.

Results:

The minimal inoculum size showing visible growth for 2 out of the 12 anaerobes in the LB broth and 9 out of the 12 anaerobes on LB-BAP was lower for the Quick anaero-system than in the BD GasPak EZ Anaerobe Container System. The mean time (±SD) required to achieve absolute anaerobic conditions of the Quick anaero-system was 17 min and 56 sec (±3 min and 25 sec).

Conclusions:

The Quick anaero-system is a simple and effective method of culturing obligate anaerobes, and its performance is superior to that of the BD GasPak EZ Anaerobe Container System.

Keywords: Anaerobic bacteria, Culture techniques, Culture media

REFERENCES

1.Loesche WJ. Oxygen sensitivity of various anaerobic bacteria. Appl Microbiol. 1969. 18:723–7.
crossref
2.Citron DM. Specimen collection and transport, anaerobic culture techniques, and identification of anaerobes. Rev Infect Dis. 1984. 6(S1):51–8.
crossref
3.Bennett GN., Hickford JG., Zhou H. Convenient anaerobic techniques, science from the supermarket shelf. Anaerobe. 2006. 12:49–51.
crossref
4.Strobel HJ. Basic laboratory culture methods for anaerobic bacteria. Methods Mol Biol. 2009. 581:247–61.
crossref
5.Imhof A., Heinzer I. Continuous monitoring of oxygen concentrations in several systems for cultivation of anaerobic bacteria. J Clin Microbiol. 1996. 34:1646–8.
crossref

Fig. 1.
Features of the Quick anaero-system: Stacked media (A), tube (B), and fully assembled Quick anaero-system with 2 media on which colony formation can be observed through disposable culture-envelope (C). The components of the Quick anaero-system and consumables are indicated by numbers as follows: (1) reusable stainless plate rack with mesh containing palladium catalyst pellet; (2) palladium catalyst pellet; (3) a new disposable anaerobic gas pack; (4) culture media plate; (5) reusable stainless tube rack; (6) tube; (7) sealer; and (8) disposable culture-envelope.
kjlm-30-133f1.tif
Table 1.
The results of comparison of the minimal inoculum size showing visible growth of 12 anaerobes when incubated in the Quick anaero-system and BD GasPak EZ Anaerobe Container System
Bacterial species Minimal inoculum size (CFU/mL) showing visible bacterial growth
LB broth LB blood agar plate
QAS BD GasPak QAS BD GasPak
Bacteroides fragilis 101 101 102 107
Bacteroides vulgatus 105 106 104 109
Bifidobacterium bifidum 104 106 104 107
Clostridium difficile 100 100 103 105
Clostridium septicum 100 100 106 107
Eubacterium limosum 100 100 103 105
Fusobacterium nucleatum subsp. polymorphum 100 100 102 104
Mobiluncus mulieris 100 100 102 100
Peptostreptococcus asaccharolyticus 100 100 104 104
Porphyromonas gingivalis 100 100 104 104
Propionibacterium acnes 100 100 104 105
Veillonella criceti 100 100 104 105

Abbreviations: LB, Luria-Bertani; QAS, Quick anaero-system; BD GasPak, BD GasPak EZ Anaerobe Container System.

TOOLS
Similar articles