Complete Blood Count Reference Values of Donated Cord Blood from Korean Neonates

Hye Ryun Lee; Sue Shin; Jong Hyun Yoon; Byoung Jae Kim; Kyu Ri Hwang; Jin Ju Kim; Eun Youn Roh

doi:10.3343/kjlm.2009.29.3.179

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Lee, Shin, Yoon, Kim, Hwang, Kim, and Roh: Complete Blood Count Reference Values of Donated Cord Blood from Korean Neonates

Original Article

Korean J Leg Med 2009;29(3):179-184.

Published online: 14 January 2009

DOI: https://doi.org/10.3343/kjlm.2009.29.3.179

Complete Blood Count Reference Values of Donated Cord Blood from Korean Neonates

Hye Ryun Lee, M.D.¹, Sue Shin, M.D.^1,^2,³, Jong Hyun Yoon, M.D.^1,^2,³, Byoung Jae Kim, M.D.^3,⁴, Kyu Ri Hwang, M.D.⁴, Jin Ju Kim, M.D.⁴, Eun Youn Roh, M.D.^1,^2,³

¹Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea

²Department of Laboratory Medicine, Seoul, Korea

³Seoul National University Boramae Hospital; Allcord, Seoul, Korea

⁴Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea

Corresponding author: Eun Youn Roh, M.D. Department of Laboratory Medicine, Seoul National University Boramae Hospital, 425 Sindaebang-dong, Dongjak-gu, Seoul 156-707, Korea Tel: +82-2-870-2603, Fax: +82-2-870-2629 E-mail: 75-crimson@hanmail.net

Revised 25 September 20083 March 2009 Accepted 4 April 2009

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background:

In the public cord blood (CB) banks, only safe CB units with adequate cell doses are processed and stored. Complete blood count (CBC) of CB is crucial for estimating total nucleated cells (TNC) and screening suitable CB units without hematologic abnormalities. We analyzed CBC parameters of the donated CB from healthy Korean neonates to establish CBC reference values.

Methods:

A total of 2,129 Korean CB units, donated and processed during the period from August 2007 to December 2007, were enrolled. We measured hemoglobin (Hb), white blood cell (WBC) count, differential count of WBC, platelets and nucleated red blood cell (nRBC) count by XE-2100 automated hematology analyzer (Sysmex, Japan), and estimated reference value of each parameter by using parametric (Mean±2SD) and/or non-parametric methods (2.5-97.5 percentile). And also, we compared the result of each parameter in relation to sex of neonates and delivery method.

Results:

Because the differences of CBC values among different subgroups were not remarkable, we established the reference intervals as follows without subgroup division: Hb, 9.0-14.4 g/dL; WBC count, 5.6-18.5×10³/μL; differential count of WBC (neutrophils, 40.8-72.4%; lymphocytes, 17.2-46.7%; monocytes, 4.9-12.8%; eosinophils, 0.7-7.0%; basophils, 0.0-1.6%); platelet, 130-287×10³/μL; nRBCs, 0.0-13.1/100 WBC.

Conclusions:

We established cord blood CBC reference values of healthy Korean neonates using a large-scale CB units. The established CBC reference values from our study will be useful as basic data for CBC interpretation and assessment of transplant suitability of donated CB.

Keywords: Donated cord blood, Reference value, CBC

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Table 1.

The cord blood CBC values (Mean±SD) in relation to sex and delivery method

	Total	Sex		P	Delivery		P
	Total	Male	Female	P	V/D	C/S	P
Hb (g/dL)	11.8±1.4	11.9±1.4	11.6±1.3	<0.001	11.9±1.4	11.4±1.4	<0.001
WBC (×10³/μL)	10.9±3.2	10.6±3.0	11.2±3.3	<0.001	11.4±3.2	9.2±2.7	<0.001
Differential count (%)
Neutrophil	57.2±8.0	56.3±7.6	58.2±8.3	<0.001	57.7±7.5	55.5±9.2	<0.001
Lymphocyte	30.9±7.4	31.5±7.1	30.2±7.6	<0.001	30.4±6.9	32.4±8.3	<0.001
Monocyte	8.5±2.0	8.7±2.1	8.3±1.9	<0.001	8.6±2.0	8.3±2.2	0.004
Eosinophil	2.9±1.7	3.1±1.7	2.7±1.6	<0.001	2.8±1.6	3.3±1.9	<0.001
Basophil	0.5±0.4	0.5±0.4	0.5±0.4	0.004	0.5±0.4	0.5±0.4	0.237
Platelet (/μL)	208±39	205±38	212±40	<0.001	214±39	192±35	<0.001
nRBCs (/100WBCs)	3.3±3.9	3.5±4.3	3.1±3.4	0.036	3.6±4.0	2.5±3.3	<0.001

Abbreviations: CBC, complete blood count; WBC, white blood cell; V/D, vaginal delivery; nRBCs, nucleated red blood cells; C/S, Cesarean section.

Table 2.

The cord blood CBC values (2.5-97.5 percentile) in relation to sex and delivery method

	Total	Sex		Delivery method
	Total	Male	Female	V/D	C/S
Hb (g/dL)	9.0-14.4	8.9-14.6	9.1-14.0	9.3-14.5	8.1-14.0
WBC (×10³/μL)	5.6-18.5	5.5-17.4	5.6-19.0	6.3-18.7	4.6-14.9
Differential count (%)
Neutrophil	40.8-72.4	40.1-71.0	41.8-73.3	42.7-72.2	37.1-73.6
Lymphocyte	17.2-46.7	17.7-47.2	15.6-45.9	17.2-45.0	17.1-50.7
Monocyte	4.9-12.8	4.9-13.0	4.9-12.5	5.0-12.7	4.7-13.8
Eosinophil	0.7-7.0	0.6-7.3	0.7-6.6	0.7-6.9	0.6-7.8
Basophil	0.0-1.6	0.0-1.5	0.0-1.7	0.0-1.6	0.0-1.4
Platelet (μL)	136-287	135-282	137-297	141-295	128-265
nRBCs (/100WBCs)	0.0-13.1	0.0-12.8	0.0-14.3	0.0-14.0	0.0-9.9

Abbreviations: See Table 1.

Table 3.

Complete blood count reference values in cord blood of Korean neonates

	Reference interval
Hb (g/dL)	9.0-14.4
WBC (×10³/μL)	5.6-18.5
Differential count (%)
Neutrophil	40.8-72.4
Lymphocyte	17.2-46.7
Monocyte	4.9-12.8
Eosinophil	0.7-7.0
Basophil	0.0-1.6
Platelet (/μL)	130-286∗
nRBCs (/100WBCs)	0.0-13.1

^∗ Estimated by using parametric method (mean±2SD); the other results using non-parametric method (2.5-97.5 percentile).

Abbreviations: See Table 1.

Table 4.

The cord blood CBC values (mean±SD or median) reported in literature

Studies	Sample size	Hb (g/dL)	WBC (×10³/μL)	Platelet (/μL)	nRBCs (/100WBC)
Pranke [10]	30	14.5±1.6	12.9±3.6	304±63	6.6±3.4
Walka [11]∗	123	15.7	14.2	265	5
Rolfo [12]∗	131	NT	NT	NT	3.1
Nam [15]	100	15.5±1.0	14.4±4.0	320±61	3.6±3.5
This study	2,129	11.8±1.4	10.9±3.2	208±39	3.3±3.9

^∗ Data presented as median values.

Abbreviations: See Table 1.

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