Gene Frequencies of Human Neutrophil Antigens 4a and 5a in the Korean Population

Tae Hee Han; Kyou Sup Han

doi:10.3343/kjlm.2006.26.2.114

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Han and Han: Gene Frequencies of Human Neutrophil Antigens 4a and 5a in the Korean Population

Original Article

Korean J Leg Med 2006;26(2):114-118.

Published online: 10 February 2006

DOI: https://doi.org/10.3343/kjlm.2006.26.2.114

Gene Frequencies of Human Neutrophil Antigens 4a and 5a in the Korean Population

Tae Hee Han, M.D.¹, Kyou Sup Han, M.D.²

¹Department of Laboratory Medicine, Inje University Sanggyep Paik Hospital, Seoul, Korea

²Department of Laboratory Medicine, College of Medicine, Seoul National University, Seoul, Korea

Received 2 November 2005 Revised 17 January 2006 Accepted 1 February 2006

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

In Korean population, antigen frequencies of HNA-1a, HNA-1b, and HNA-2a are determined using a serological and genotyping method. However, no study has been done to assess the gene frequencies of HNA-4a and HNA-5a. It has been reported that the antibody against HNA-4a is associated with alloimmune neutropenia and autoimune neutropenia; however, there is no confirmed clinical report on anti-HNA-5a-related disorders. The aim of this study was to determine HNA-4a and HNA-5a gene frequencies among the Korean population.

Methods

Genotyping of HNA-4a and HNA-5a genes of 110 healthy and unrelated Korean donors was performed using a polymerase chain reaction with sequence-specific primers and an allele-specific restriction enzyme analysis.

Results

We found that the gene frequencies of HNA-4a and HNA-5a were 0.99 (107/110) and 0.96 (3/110), respectively, among the Korean population. But only the ones of the latter was significantly higher (P<0.01) than in the one of Caucasians.

Conclusions

The gene frequencies of HNA-4a and HNA-5a were determined. We also identified an individual who was the HNA-5a-negative homozygote for the granulocyte panel that could be used for anti-HNA-5a antibody identification.

Keywords: HNA-4a antigen, HNA-5a antigen, Gene frequency

References

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Fig. 1.

HNA-4a genotyping by PCR-SSP. Lanes 1 and 6 show a DNA ladder marker (Bioneer, Daejeon, Korea). The amplification products (439 bp) of the internal control (HGH gene) are present in each lane. Lanes 2, 3, 7, and 8 contain HNA-4a-positive-specific reactions. Lanes 4, 5, 9, and 10 contain HNA-4a-negative-specific reactions. Lanes 2 and 4 are positive controls for HNA-4a-positive and HNA-4a-negative samples, respectively, and lanes 3 and 5 are negative controls for HNA-4a-positive and HNA-4a-negative samples, respectively. Lanes 7 and 8 contain an HNA-4a-positive homozygote sample and lanes 9 and 10 contain an HNA-4a-heterozygote sample.

Fig. 2.

HNA-5a genotyping by Bsp1286I allele-specific restriction enzyme analysis (ASRA). Lane 1 shows a DNA ladder marker (Bioneer, Daejeon, Korea); lane 2 shows an undigested 709 bp PCR product of α_L chain of β₂ integrin cDNA; lane 3 shows an HNA-5a-positive homozygote sample (297 bp, 217 bp, and 195 bp); lane 4 shows an HNA-5a heterozygote sample (412 bp, 297 bp, 217 bp, and 195 bp); and lane 5 shows an HNA-5a-negative homozygote sample (412 bp, and 297 bp).

Table 1.

HNA-4a and HNA-5a genotype frequencies and gene frequencies

		HNA-4a	HNA-5a
Genotype frequencies	+/+	0.973 (107/110)	0.927 (102/110)
	+/-	0.027 (3/110)	0.064 (7/110)
	−/-	0.000 (0/110)	0.009 (1/110)
Allele frequencies	+	0.986	0.959
	−	0.014	0.041

Table 2.

Reported human neutrophil antigen (HNA) gene frequencies

HNA system	Korean	Chinese	Japanese	Caucasian	References
HNA-1a	0.52	0.68	0.65	0.35	7–13
HNA-1b	0.48	0.32	0.35	0.65	7–13
HNA-1c	nk	0.0	0.0	0.02–0.03	7–13
HNA-2a	0.62	0.91	0.66	0.83	7–13
HNA-3a	nk	nk	nk	0.66	13
HNA-4a	0.99^*	nk	nk	0.91	5, 6, 13
HNA-5a	0.96^*	nk	nk	0.82	5, 13

^* gene frequencies determined in the present study.

Abbreviation: nk, not known.

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