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Choi, Kim, Ahn, Hong, Lee, Kim, Kim, Nam, and Yim: The Wound Healing Effect of a Silk Fibroin Film on Cutaneous Burn of Hairless Mice
Original Article
Journal of the Korean Surgical Society

Journal of the Korean Surgical Society 2010; 79(6): 421-427.

Published online: 31 December 2010

DOI: https://doi.org/10.4174/jkss.2010.79.6.421

The Wound Healing Effect of a Silk Fibroin Film on Cutaneous Burn of Hairless Mice

Young Hee Choi, M.D., Ph.D., Min Gyu Kim, M.D., Dong Hyun Ahn, M.D., Soo Hee Hong, M.D.1, Jae-Yong Lee, Ph.D.1, Hae Sung Kim, M.D.2, Heung Cheol Kim, M.D.3, Sang Yong Nam, Ph.D.4, Garbeen Yim, Ph.D.5

Department of Pathology, College of Medicine, Hallym University, Chuncheon, Korea.

1Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Korea.

2Department of Surgery, College of Medicine, Hallym University, Chuncheon, Korea.

3Department of Radiology, College of Medicine, Hallym University, Chuncheon, Korea.

4Division of Natural Science, SahmYook University, Seoul, Korea.

5FineCo Ltd., Chuncheon, Korea.

Corresponding author (biogra@hallym.or.kr)

Received 25 May 2010       Accepted 8 October 2010

Copyright © 2010 The Korean Surgical Society

Abstract

Purpose

The aim of this study was to examine the effects of silk fibroin film on wound healing of cutaneous burn in hairless mice by using microscopic findings and stem cell markers (nestin, cytokeratin 15) and ki-67 proliferation marker.

Methods

Each mouse received two burns at the dorsal area by applying a metal rod heated with boiling water. Burn wound sites were dressed with Silk Fibroin Film and duoderm (SF group), Aquacel hydrofiber and duoderm (AC group) and duoderm only (Control group). All groups were covered externally with duodermas adhesive bands. Those mice were sacrificed at zero, two, seven, fourteen and twenty one days after burn. Histological findings and immunohistochemical staining for stem cell markers were observed.

Results

In SF group, inflammatory cell infiltration, formation of granulation tissue and inflammatory foci are greater than in AC and control group. Those factors appear to enhance mesenchymal stem cell markers such as nestin. Finally mesenchymal tissue regeneration was enhanced. In addition, the length of ki-67 expressed re-generating epithelium, which appeared to be associated with epithelial regeneration, was the longest in SF group.

Conclusion

The results show that the wound healing effect of SF is the best among other treatment materials including AC in the experimental group and duoderm in the control group through mesenchymal regeneration and epithelial regeneration which are essential factors for wound healing.

Keywords: Hairless mice, Silk fibroin film, Burn, Wound healing, Stem cell

Figures and Tables

Fig. 1
Immunohistochemical expression of nestin in granulation tissue 7 days after burn. The long and thin arrow indicates necrotic tissue after burn. Nestin presents in the capillaries and peripheral nerve bundles (short and thick arrow) in the granulation tissue under the necrotic tissue (×40).
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Fig. 2
Immunohistochemical expression of keratin 15 in regenerating epithelium 2 days after burn. A shows the distal part of regenerating epithelium without keratin 15 expression. B shows the proximal part of regenerating epithelium with keratin 15 expression of superficial layer. C shows the normal epidermis with keratin 15 expression of the full thickness skin except basal layer (×40, Inset: ×100).
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Fig. 3
Immunohistochemical expression of Ki-67 in regenerating epithelium on 2 days after burn. A shows the distal part of regenerating epithelium with sparse Ki-67 expression. B shows the proximal part of regenerating epithelium with Ki-67 expression of 2~3 basal layers. C shows the normal epidermis with Ki-67 expression of basal layer (×40, Inset: ×100).
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Table 1
Histologic changes in dressed wounds at 2, 7, 14 and 21 days after burn
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Control represents the animals with burn wound dressed with duoderm only. AC group represents the animals with burn wound dressed with aqua cell and duoderm. SF group represent the animals with burn wound dressed with Silk fibroin film and duoderm. *Duncan's multiple range test: Means with the same letter are not significantly different. Means with a different letter are significantly different (P<0.05). Example = A, B are significantly different (P<0.05). Cellularity and Amount of granulation tissue represent the percentage in total area of wound.

Table 2
The length of the expression of CK15 in regenerating epithelium
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Control represents the animals with burn wound dressed with duoderm only. AC group represents the animals with burn wound dressed with aqua cell and duoderm. SF group represents the animals with burn wound dressed with Silk fibroin film and duoderm. *Duncan's multiple range test: Means with the same letter are not significantly different. Means with a different letter are significantly different (P<0.05). Example = A, B are significantly different (P<0.05).

Table 3
Expression of Ki-67 in regenerating epithelium.
jkss-79-421-i003

Control represent the animals with burn wound dressed with duoderm only. AC group represent the animals with burn wound dressed with aqua cell and duoderm. SF group represent the animals with burn wound dressed with Silk fibroin film and duoderm. *Duncan's multiple range test: Means with the same letter are not significantly different. Means with a different letter are significantly different (P<0.05). Example = A, B are significantly different (P<0.05).

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