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Journal List > J Korean Surg Soc > v.79(1) > 1011143

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Choi, Kim, Ahn, Cho, Hong, Lee, Lee, and Kim: Immunohistochemical Expression of Stem Cell Markers during the Wound Healing Process of Cutaneous Burn
Original Article
Journal of the Korean Surgical Society

Journal of the Korean Surgical Society 2010; 79(1): 1-7.

Published online: 25 June 2010

DOI: https://doi.org/10.4174/jkss.2010.79.1.1

Immunohistochemical Expression of Stem Cell Markers during the Wound Healing Process of Cutaneous Burn

Young Hee Choi, M.D., Min Gyu Kim, M.D., Dong-Hyun Ahn, M.D., Seong Jin Cho, M.D., Soo Hee Hong1, Jae-Yong Lee, Ph.D.1, Jin Won Lee, M.D.2, Hae Sung Kim, M.D.2

Department of Pathology, College of Medicine, Hallym University, Chuncheon, Korea.

1Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Korea.

2Department of Surgery, College of Medicine, Hallym University, Chuncheon, Korea.

Corresponding author (biogra@hallym.or.kr)

Received 5 January 2010       Accepted 23 March 2010

Copyright © 2010 The Korean Surgical Society

Abstract

Purpose

A cutaneous wound healing requires a well-orchestrated integration of the complex biological and molecular events of cell migration and proliferation, extracellular matrix deposition, angiogenesis and remodeling. Finally, skin regeneration is the main goal. Stem cells are self-renewing multipotent progenitors with the broadest developmental potential in a given tissue at a given time. The aim of this study was to examine the role of stem cells during the wound healing process of cutaneous burn in hairless mice by using immunohistochemical stainings (nestin, cytokeratin 15 and CD31).

Methods

Each mouse received 2 burns at the dorsal area by applying a metal stick heated in boiling water. Burn wound sites were dressed with duoderm. The mice were sacrificed at 0, 2, 7, 14 and 21 days after burn. Histological findings and immunohistochemical expression for stem cell markers were observed.

Results

Nestin was expressed in the stromal cells beneath the epidermis, hair follices, dermal cysts and endothelial cells. Cytokeratin 15 was expressed in the epidermis except in basal cells. On 7 and 14 days after burn, the regenerated epidermis didn't express cytokeratin 15. CD31 was expressed in the endothelial cells on 7 and 14 days after burn. The amount of nestin expression was the highest.

Conclusion

Our results showed that nestin may have various effects on burn wound healing. Cytokeratin 15 was expressed before burn and after burn. It is likely that other cytokeratin may stimulate epithelial regeneration. CD31 may act in vascular regeneration during burn healing.

Keywords: Hairless mouse, Burn, Wound healing, Stem cell

Figures and Tables

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Fig. 1
Structures of normal and burned skin in hairless mice. (A) Normal skin showed the epidermis and dermis. (B) After burn, epidermis and most dermis were damaged. However, the lower part of dermal cyst was still intact as second-degree burn wound demonstrated (H&E, ×40).

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Fig. 2
Immunohistochemistry for nestin. Expression of nestin protein is present in the cells below the epidermis (A), the outer cells of epidermal cyst (B) and endothelial cells of the granulation tissue (C).

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jkss-79-1-g003
Fig. 3
Immunohistochemistry for cytokeratin 15. Expression of cytokeratin 15 is present in suptrabasal area of epidermis (A). At 7 days after burn, the expression of cytokeratin 15 was not present in regenerated epidermis (arrow).

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jkss-79-1-g004
Fig. 4
Immunohistochemistry for CD31. Expression of CD31 is present in endothelial cells in 7 days after burn.

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Table 1
Histologic changes in dressed wounds at 2, 7, 14 and 21 days after burn
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*Duncan's multiple range test: Means with the same letter are not significantly different. Means with the different letter are significantly different (P<0.05).

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