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Moon, Moon, and Park: Detection and Typing of Human Papillomavirus in Anal Condyloma Acuminatum of HIV-positive Patients
Original Article
Journal of the Korean Surgical Society

Journal of the Korean Surgical Society 2010; 78(2): 111-115.

Published online: 19 February 2010

DOI: https://doi.org/10.4174/jkss.2010.78.2.111

Detection and Typing of Human Papillomavirus in Anal Condyloma Acuminatum of HIV-positive Patients

Suk-Bae Moon, M.D., Ph.D., Sang-Hee Moon, M.D., Kyu-Joo Park, M.D., Ph.D.

Department of Surgery, Seoul National University College of Medicine, Seoul, Korea.

Corresponding author (kjparkmd@plaza.snu.ac.kr)

Received 20 October 2009       Accepted 18 November 2009

Copyright © 2010 The Korean Surgical Society

Abstract

Purpose

Anal condyloma is an epithelial proliferative lesion caused by human papillomavirus (HPV) infection. The present study analyzed the HPV types detected in HIV (+) Korean anal condyloma using PCR-based DNA microarray.

Methods

DNA was extracted from the condyloma tissue of 17 patients including 9 HIV (+) patients (M:F=15:2, mean age 35 years, 22~59 years). The 1st PCR was performed with a general primer on L1 region, and nested PCR on the products of the 1st PCR. PCR products were hybridized with a DNA chip.

Results

Fourteen patients (9 HIV (+), 5 HIV (-)) showed positive HPV DNA. Overall, type 6 was the most common (N=11), and type 11 (N=6), type 53 (N=3) in order. Among HIV (+) patients, type 6 was also the most common (N=7), then type 11 (N=5) and type 53 (N=3). In contrast to the HIV (-) patients, 5 patients (55.6%) proved to have multiple infections in HIV (+) patients (2 double, 2 triple, 1 quadruple infection). Four of 9 HIV (+) patients (44.5%) showed co-infection with high-risk HPV.

Conclusion

Multiple infection and co-infection with high-risk types are more prevalent in HIV (+) condyloma patients compared to HIV (-) patients. HPV types on HIV (+) male anal condyloma are similar to those detected in the Korean female uterine cervix.

Keywords: Anal condyloma acuminatum, Human papillomavirus, Human immunodeficiency virus, Polymerase chain reaction, DNA microarray

Figures and Tables

Fig. 1
Electrophoresis of the 1st PCR products (450 bp) and the nested PCR products (150 bp) (p = positive control, n = negative control).
jkss-78-111-g001
Fig. 2
DNA chip scanning of patients No. 2, 5, 8.
jkss-78-111-g002
Fig. 3
HPV type distribution in anal condyloma according to the HIV status (* = other type).
jkss-78-111-g003
Fig. 4
Multiplicity of the HPV infection according to the HIV status.
jkss-78-111-g004
Table 1
Patients with positive HPV DNA and the resultant HPV typing
jkss-78-111-i001

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