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Yook and Sung: In Vitro Antibacterial Efficacy of Vicryl and PDS Plus Antibacterial Suture
Original Article
Journal of the Korean Surgical Society

Journal of the Korean Surgical Society 2010; 78(1): 1-6.

Published online: 8 January 2010

DOI: https://doi.org/10.4174/jkss.2010.78.1.1

In Vitro Antibacterial Efficacy of Vicryl and PDS Plus Antibacterial Suture

Jeong Hwan Yook, M.D., Heungsup Sung, M.D.1

Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

1Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

Corresponding author (jhyook@amc.seoul.kr)

Received 25 August 2009       Accepted 19 October 2009

Copyright © 2010 The Korean Surgical Society

Abstract

Purpose

Surgical site infection (SSI) is the most common nosocomial infection in surgical patients, and this accounts for approximately 17% of all hospital-acquired infections. Suture materials are possibly significant sources of SSI. This study aims to evaluate the in vitro antibacterial efficacy of Vicryl and PDS plus antibacterial suture coating with triclosan against bacteria.

Methods

Vicryl and PDS plus antibacterial suture coating with and without triclosan were tested for in vitro efficacy against methicillin-susceptible Staphylococcus aureus, methicillin-resistant S. aureus, methicillin-resistant Staphylococcus epidermidis, Escherichia coli by a zone of inhibition assay and test of bacterial adhesion and viability.

Results

Vicryl and PDS plus antibacterial suture coating with triclosan demonstrated activity against all tested bacteria in vitro. Evaluations by a zone of inhibition assay and test of bacterial adhesion and viability show the antibacterial activity compared with untreated sutures. Pretreatment of surgical sutures with fetal bovine serum did not diminish antibacterial activity of the triclosan-coated sutures compared with non-coated sutures (P<0.01).

Conclusion

Vicryl and PDS plus antibacterial suture reduced in vitro colonization of several strains of bacteria compared with untreated control sutures.

Keywords: Surgical site infection, Suture, Triclosan, Antibacterial

Figures and Tables

Fig. 1
Zone of inhibition of 2-0 Vicryl, 2-0 Vicryl Plus Antibacterial, 2-0 PDS II, and 2-0 PDS Plus Antibacterial sutures (left-to-right) against (A) methicillin-susceptible Staphylococcus aureus (MSSA) ATCC 25923, (B) methicillin-resistant S. aureus (MRSA) ATCC 43300, (C) Staphylococcus epidermidis, and (D) extended-spectrum β-lactamase (ESBL) producing Escherichia coli. Antibacterial suture materials showed the inhibition of bacterial growth.
jkss-78-1-g001
Fig. 2
Zone of inhibition of 4-0 Vicryl, 4-0 Vicryl Plus Antibacterial, 4-0 PDS II, and 4-0 PDS Plus Antibacterial sutures (left-to-right) against (A) MSSA ATCC 25923, (B) MRSA ATCC 43300, (C) S. epidermidis, and (D) ESBL producing E. coli. Antibacterial suture materials showed the inhibition of bacterial growth.
jkss-78-1-g002
Fig. 3
Mean microbial recovery from Vicryl and Vicryl Plus Antimicrobial sutures exposed to bacterial inoculum for 60 minutes. There were significant differences in bacterial adherence and viability (P<0.01).
jkss-78-1-g003
Fig. 4
Impact of 20% feta bovine serum (FBS) on MRSA ATCC 43300 and E. coli mean microbial recovery from Vicryl and Vicryl Plus Antimicrobial sutures. There were significant differences in bacterial adherence and viability (P<0.01).
jkss-78-1-g004
Table 1
In vitro diffusion from 2-0 Vicryl with triclosan and 2-0 PDS with triclosan
jkss-78-1-i001

*ATCC = American type culture collection; †ESBL = extended-spectrum β-lactamase.

Table 2
In vitro diffusion from 2-0 Vicryl with triclosan and 2-0 PDS with triclosan
jkss-78-1-i002

*ATCC = American type culture collection; †ESBL = extended-spectrum β-lactamase.

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