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Abstract
Purpose
To investigate the effects of tetrahydrozoline (THZ) on the survival of cultured human trabecular meshwork cells (HTMC) and the permeability of HTMC monolayer.
Methods
Primary cultured HTMC were exposed to an adrenergic agonist (0.01, 0.1, 1.0 or 10 μ M THZ) for 1 day and 3 days. Carboxyfluorescein permeability through the HTMC monolayer was measured using Transwell. Cellular viability and nitric oxide (NO) production were assessed using MTT and Griess assays, respectively.
References
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Figure 1.
Effect of tetrahydrozoline on the survival of con-fluently cultured trabecular meshwork exposed for 1 day. Tertrahydrozoline did not affect on the survival significantly compared to non-exposed control ( p > 0.05).
Figure 2.
Effect of tetrahydrozoline on the survival of con-fluently cultured trabecular meshwork exposed for 3 days. Tertrahydrozoline did not affect on the survival significantly compared to non-exposed control ( p > 0.05).
Figure 3.
Effects of tetrahydrozoline on the permeability of car-boxyfluorescin through the trabecular meshwork cell monolayer exposed for 1 day. 1.0, 10.0 μ M tetrahydrozoline decreased the permeability of carboxyfluorescin significantly compared to non-exposed control (* p < 0.05). Carboxyfluorescein intensity of outer chamber normalized to the mean value obtained using non-exposed control (permeability 100%).
Figure 4.
Effects of tetrahydrozoline on the permeability of car-boxyfluorescin through the trabecular meshwork cell monolayer exposed for 3 days. 0.1, 1.0, and 10.0 μ M tetrahydrozoline de-creased the permeability of carboxyfluorescin significantly com-pared to non-exposed control (* p < 0.05). Carboxyfluorescein intensity of outer chamber normalized to the mean value obtained using non-exposed control (permeability 100%).
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