Journal List > J Korean Ophthalmol Soc > v.58(5) > 1010759

Jung, Lee, Park, Yu, Kim, and Lee: Amoebicidal Effect of Nephrite-containing Contact Lens Storage Case

Abstract

Purpose

To compare the amoebicidal effects of nephrite containing contact lens (CL) storage cases with conventional CL storage cases.

Methods

Acanthamoeba lugdunensis were inoculated onto 5% nephrite containing CL storage cases as well as conventional CL storage cases both with and without silicone hydrogel contact lenses (SHCLs). Then the amount of Acanthamoeba proliferation on CL storage cases and the number of adherent Acanthamoeba on SHCLs were determined and compared. The effects of multipurpose solution (MPS) with and without 1% or 5% nephrite solution on Acanthamoeba adhesion were analyzed.

Results

Nephrite containing CL storage cases showed more inhibitory effects on Acanthamoeba proliferation (p = 0.02) and significantly reduced the number of adherent Acanthamoeba on SHCLs compared with conventional CL storage cases, regardless of SHCLs generation (p = 0.001, p = 0.001 and p < 0.001, respectively). The number of adherent Acanthamoeba on the first generation of SHCLs was significantly reduced by MPS with 1% and 5% nephrite solutions (p = 0.03 and p = 0.004, respectively), but the numbers for the second and third generation SHCLs were not.

Conclusions

Nephrite could be used as a new additive component for CL storage cases and multipurpose solutions to improve the disinfection effects on Acanthamoeba.

Figures and Tables

Figure 1

Number of Acanthamoeba on contact lens (CL) storage cases. CL storage cases containing 5% nephrite significantly reduced the numbers of Acanthamoeba compared with traditional CL storage cases.

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Figure 2

Phase contrast microscopic images of adherent Acanthamoeba trophozites. (A-F) These pictures show the decreased number of Acanthamoeba adhering to all generations of silicone hydrogel contact lenses in contact lens (CL) storage cases containing 5% nephrite.

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Figure 3

The number of Acanthamoeba adhering to contact lenses. The number of Acanthamoeba adhering to contact lenses in contact lens storage case containing 5% nephrite was significantly reduced compared with traditional contact lens storage case regardless of the generation of silicone hydrogel contact lenses.

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Figure 4

Phase contrast microscopic images of adherent Acanthamoeba trophozoites. (A-C) The Opti-free express contatining 1% or 5% nephrite solutions significantly reduced the adherence of Acanthamoeba trophozoites on Air-Optix compared with Opti-free express only.

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Figure 5

Relationship of Acanthamoeba trophozoites adherence among contact lenses treated with multipurpose solution with and without 1% or 5% nephrite solution. In case of Air Opitx, the effect of Opti-free express containing nephrite solutions on disinfection in terms of Acanthamoeba trophozoites were superior to that of Opti-free express only. Acanthamoeba trophozoite did not adhere to Oasys and Biofinity regardless of multipurpose solution with and without nephrite solutions

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