The Effect of Anti-inflammatory Agents on the Permeability of Trabecular Meshwork Cell Monolayers

Hye Ri Kang; Jung Hoon Lee; Jae Woo Kim

doi:10.3341/jkos.2017.58.10.1155

Journal List > J Korean Ophthalmol Soc > v.58(10) > 1010649

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Kang, Lee, and Kim: The Effect of Anti-inflammatory Agents on the Permeability of Trabecular Meshwork Cell Monolayers

Original Article

J Korean Ophthalmol Soc 2017;58(10):1155-1159.

Published online: 25 September 2017

DOI: https://doi.org/10.3341/jkos.2017.58.10.1155

The Effect of Anti-inflammatory Agents on the Permeability of Trabecular Meshwork Cell Monolayers

Hye Ri Kang, MD¹, Jung Hoon Lee, MD², Jae Woo Kim, MD, PhD^1,

¹Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea

²Catholic Union Eye Clinic, Daegu, Korea

Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Daegu Catholic University Medical Center, #33 Duryugongwon-ro 17 gil, Nam-gu, Daegu 42472, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133 E-mail: jwkim@cu.ac.kr

Received 13 October 2016 Revised 5 January 2017 Accepted 18 September 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To compare the effects of anti-inflammatory agents, specifically bromfenac, loteprednol, and prednisolone, on the permeability of cultured human trabecular meshwork cell (HTMC) monolayers.

Methods

HTMCs were cultured until confluency in the inner chamber of Transwell, then exposed to 1/1,000 or 1/500 diluted commercial 0.1% bromfenac, 0.5% loteprednol, and 1% prednisolone for 24 hours. The permeabilities of carboxyfluorescein through the HTMC monolayer were measured with a spectrofluorometer after 2 hours in the outer chamber. Cellular viabilities were assessed with an 3-[4,5– dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.

Results

Bromfenac and loteprednol diluted at 1/1,000 or 1/500 did not significantly affect the cellular survival (p > 0.05). Bromfenac did not affect the permeability via the HTMC monolayer (p > 0.05) and loteprednol decreased the permeability (p < 0.05). In addition, 1/2,000 prednisolone also decreased the permeability (p < 0.05).

Conclusions

Among the anti-inflammatory agents, the non-steroidal anti-inflammatory agent bromfenac did not affect the permeability, while loteprednol and prednisolone decreased the permeability through the HTMC monolayer. Thus, loteprednol and prednisolone may decrease the trabecular outflow.

Keywords: Bromfenac, Loteprednol, Permeability, Prednisolone, Trabecular meshwork cells

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Figure 1.

Effect of bromfenac sodium (BF) and loteprednol etabonate (LE) on the survival of human trabecular meshwork cells in tissue culture. Both bromfenac sodium and loteprednol did not affect the survival of trabecular meshwork cells compared to non-exposed controls (X= 1/1,000 dilution).

Figure 2.

Effect of prednisolone acetate (PD) on the survival of human trabecular meshwork cells in tissue culture. Exposure to 1/1,000 or 1/500 diluted prednisolone decreased the survival of trabecular meshwork cells compared to non-ex-posed controls (^* p < 0.05) (X = 1/1,000 dilution).

Figure 3.

Effects of bromfenac sodium (BF), loteprednol ta-bonate (LE), or prednisolone acetate (PD) on the permeability of carboxyfluorescin through the trabecular meshwork cell monolayer. Both loteprednol and prednisolone decreased the permeabilty of carboxyfluorescein significantly compared control using phosphated buffered saline (PBS) (^* p < 0.05). Carboxyfluorescein intensity of outer chamber normalized to the mean value obtained using PBS (permeability 100%) (X= 1/1,000 dilution).

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