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Abstract
Purpose
To evaluate the immediate effects of 3% diquafosol ophthalmic solution on tear MUC5AC concentration and corneal wetting property in rabbit eyes.
Methods
Six New Zealand white rabbits were used in the present study. Fifteen minutes after instilling 50 μL of 3% diquafosol into the right eye of each rabbit and 50 μL of saline into the left eye, corneal wetting property, tear MUC5AC concentrations and the area of periodic acid-Schiff (PAS)-stained conjunctival goblet cells were evaluated under general anesthesia using conjunctival impression cytology. Corneal wetting property was evaluated by measuring the duration from when the image of the microscopic light beam was clear on the corneal surface immediately after blinking to when the image began to blur.
Results
The mean time of corneal wetting property was 86.40 (± 17.90) seconds in the diquafosol group and 49.00 (± 6.35) seconds in the control group. There was a significant difference between the two groups (p = 0.043). The mean concentration of tear MUC5AC was significantly higher in the diquafosol group (18.21 ± 1.52 ng/mL) than the control group (12.75 ± 1.82 ng/mL; p = 0.028). Conjunctival impression cytology showed the area of PAS-stained conjunctival goblet cells was significantly lower in the diquafosol group (23.17 ± 0.05%) than the control group (32.49 ± 0.08%; p = 0.028).
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Figure 1.
The method to measure corneal wetting property. (A) The images of microscopic light and corneal disk rings on the cornea are clear. (B) The images start to get blurred.
Figure 2.
Comparison of corneal wetting property between normal saline and 3% diquafosol tetrasodium (n = 6). *p = 0.043 by Wilcoxon signed rank test.
Figure 3.
Comparison of tear MUC5AC concentration 15 minutes after instillation of normal saline or 3% diquafosol tetrasodium (n = 6). *p = 0.028 by Wilcoxon signed rank test.
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