Effect of Bimatoprost on the Permeability of Trabecular Meshwork Cell Monolayer

Bi Chang; Jae Woo Kim

doi:10.7469/JKOS.2015.56.04.586

Journal List > J Korean Ophthalmol Soc > v.56(4) > 1010249

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Chang and Kim: Effect of Bimatoprost on the Permeability of Trabecular Meshwork Cell Monolayer

Original Article

J Korean Ophthalmol Soc 2015;56(4):586-591.

Published online: 7 September 2015

DOI: https://doi.org/10.7469/JKOS.2015.56.04.586

Effect of Bimatoprost on the Permeability of Trabecular Meshwork Cell Monolayer

Bi Chang, MD, Jae Woo Kim, MD, PhD

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea

￭ Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Daegu Catholic University Medical Center, #33 Duryugongwon-ro 17-gil, Nam-gu, Daegu 705-718, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133 E-mail: jwkim@cu.ac.kr

Received 2 October 201412 December 2014 Accepted 7 March 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

초록

Purpose:

To investigate the effects of bimatoprost on the permeability of cultured human trabecular meshwork cells (HTMC) monolayer.

Methods:

HTMCs were cultured until confluency in the inner Transwell chamber and then exposed to benzalkonium chloride, bri-monidine, latanoprost or bimatoprost for 1 week. Carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer after 2 hours in the outer chamber. Cellular viability was assessed using the MTT assay.

Results:

Each drug diluted at 1/1000X did not affect the cellular survival ( p > 0.05). Brimonidine, latanoprost and bimatoprost did not affect the carboxyfluorescein permeability through the HTMC monolayer ( p > 0.05). The carboxyfluorescein permeability was not different between latanoptost and bimatoprost after 1 week of exposure ( p > 0.05).

Conclusions:

Bimatoprost, a drug known to increase trabecular outflow, does not affect the carboxyfluorescein permeability through the HTMC monolayer. Thus, the effect on the trabecular outflow of bimatoprost may not be significant.

Keywords: Bimatoprost, Carboxyfluorescein, Permeability, Trabecular meshwork cells

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Figure 1.

Photograph of confluently cultured trabecular meshwork cells in monolayer (×100).

Figure 2.

Effects of BAC, BMD, LAT and BIM on the survival of trabecular meshwork cells. Each drug did not decrease cellular viability significantly ( p > 0.05). BAC = benzalkonium chloride; BMD = brimonidine; LAT = latanoprost; BIM = bimatoprost.

Figure 3.

Effects of BAC, BMD, and BIM on the permeability of carboxyfluorescin through the trabecular meshwork cell monolayer. Each drug did not affect on the permeabilty of carboxyfluorescein significantly compared control using PBS ( p > 0.05). Carboxyfluorescein intensity of outer chamber normalized to the mean value obtained using PBS (permeability 100%). PBS = phosphate buffered saline; BAC = benzalkonium chloride; BMD = brimonidine; BIM = bimatoprost.

Figure 4.

Comparison of the permeability of carboxyfluorescin through the trabecular meshwork cell monolayer between LAT and BIM. There is no significant difference of the permeabilty of carboxyfluorescein between LAT and BIM ( p > 0.05). Carboxyfluorescein intensity of outer chamber normalized to the mean value obtained using PBS (permeability 100%). PBS = phosphate buffered saline; LAT = latanoprost; BIM = bimatoprost.

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