Abstract
Purpose
Resveratrol exerts cytoprotective or cytotoxic effects according to cell type. This study was performed to evaluate the effects of resveratrol on the survival of cultured human Tenon’s capsule fibroblasts (HTFBs).
Methods
Primarily cultured HTFBs were exposed to 0, 10, or 100 μ M resveratrol for 3 days. Cellular survival was assessed us-ing the MTT assay and degree of apoptosis was analyzed with flow cytometry using annexin-V/propidium iodide double staining.
Results
Resveratrol decreased the survival of HTFBs after exposure to 10 μ M ( p = 0.04). In flow cytometric analysis, 10 μ M re-sveratrol did not affect the degree of apoptosis ( p = 0.89), but 100 μ M resveratrol increased the degree of apoptosis ( p = 0.003). Both 10 and 100 μ M resveratrol did not affect the degree of necrosis ( p = 0.74, 0.33).
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Figure 1.
Effects of resveratrol on the survival of human Tenon's capsule fibroblasts. Resveratrol decreased cellular survival significantly in a dose-dependent manner. * p < 0.05.
![jkos-56-1268f1.tif](/upload/SynapseXML/0035jkos/thumb/jkos-56-1268f1.gif)
Figure 2.
Flow cytometric analysis of apoptosis using annexin-PI double staining. Cells in quadrant B1, B2, B3, B4 rep-resents necrotic cells, late apoptotic cells, living cells and early apoptotic cells, respectively. (A) Unstained control. (B) Exposed to 100 μ M resveratrol. FITC = fluorescein isothiocyanate; PI = propidium iodide.
![jkos-56-1268f2.tif](/upload/SynapseXML/0035jkos/thumb/jkos-56-1268f2.gif)
Figure 3.
Flow cytometric analysis of apoptosis using annex-in-PI double staining. 100 μ M resveratrol increased the degree of apoptosis significantly compared to non-exposed control. PI = propidium iodide. * p < 0.05.
![jkos-56-1268f3.tif](/upload/SynapseXML/0035jkos/thumb/jkos-56-1268f3.gif)