Abstract
Purpose
To evaluate the effect of olopatadine and ketotifen to stabilize mast cells using human umbilical cord blood-de-rived mast cells (hCBMCs).
Methods
Using cultured hCBMCs, we divided the cells into the Ketotifen fumarate treatment group, the Olopatadine hydrochloride treatment group, the positive control group, and the negative control group. The histamine release inhibition rate was then observed.
Results
Ketotifen and olopatadine both showed the highest inhibition rate of histamine release at a concentration of 10-3.5 M (Ketotifen, 48% and Olopatadine, 62%). The histamine release inhibition rate of olopatadine was 28% at a concentration of 10-5.5 M, but ketotifen demonstrated a low histamine release inhibition rate at the same concentration. Ketotifen and olopatadine showed no histamine release inhibition at concentrations of 10-2~10-2.5 M, and 10-6 M.
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Figure 1.
(A) Histamine ELISA kit (H5085, US Biological, Swampscott, Massachusetts, USA) (B) ELISA reader (TECAN. Männedorf, Switzerland).
![jkos-55-278f1.tif](/upload/SynapseXML/0035jkos/thumb/jkos-55-278f1.gif)
Figure 2.
Inhibition of histamine release from human umbilical cord blood-derived cultured mast cells (hCBMCs) (% reduction from positive control) as a function of ketotifen fumarate concentration.
![jkos-55-278f2.tif](/upload/SynapseXML/0035jkos/thumb/jkos-55-278f2.gif)