Effect of Bevacizumab and Ranibizumab on the Expression of eNOS in Trabecular Meshwork Cells

Se Eun Kim; Jae Woo Kim

doi:10.3341/jkos.2014.55.8.1208

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Kim and Kim: Effect of Bevacizumab and Ranibizumab on the Expression of eNOS in Trabecular Meshwork Cells

Original Article

J Korean Ophthalmol Soc 2014;55(8):1208-1212.

Published online: 31 August 2014

DOI: https://doi.org/10.3341/jkos.2014.55.8.1208

Effect of Bevacizumab and Ranibizumab on the Expression of eNOS in Trabecular Meshwork Cells

Se Eun Kim, MD, Jae Woo Kim, MD, PhD

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea

Address reprint requests to Jae Woo Kim, MD, PhD, Department of Ophthalmology, Daegu Catholic University Medical Center, #33 Duryugongwon-ro 17-gil, Nam-gu, Daegu 705-718, Korea, Tel: 82-53-650-4728, Fax: 82-53-627-0133 E-mail: jwkim@cu.ac.kr

Received 25 January 2014 Revised 14 February 2014 Accepted 24 June 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To compare the effects of bevacizumab and ranibizumab on the expression of eNOS in cultured human trabecular meshwork cells (HTMC).

Methods

Primarily cultured HTMC were exposed to 0.5 mg/mL bevacizumab and ranibizumab using 1% serum-containing media for 30 minutes. Expression of eNOS mRNA was assessed with RT-PCR. Additionally, after exposure to 20 ng/mL of vascular endothelial growth factor (VEGF) and 0.5 mg/mL bevacizumab and ranibizumab, the production of nitric oxide was assessed with Griess assay.

Results

VEGF increased the production of nitric oxide in HTMC. Bevacizumab and ranibizumab decreased the expression of eNOS mRNA and production of nitric oxide (p < 0.05) in HTMC. The decrease in eNOS mRNA expression and production of nitric oxide was not significant between bevacizumab and ranibizumab (p > 0.05).

Conclusions

In HTMC, both bevacizumab and ranibizumab decreased the expression of eNOS mRNA with no significant difference observed between the two drugs.

Keywords: Bevacizumab, eNOS, Nitric oxide, Ranibizumab, Trabecular meshwork

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Figure 1.

Effects of vascular endothelial growth factor (VEGF) on the production of nitric oxide in trabecular mesh- work cells. VEGF increased Nitric oxide (NO) production (^*p < 0.05). Both Akt inhibitor (A) and wortmanin (W) inhibit VEGF-induced NO production (^†p < 0.05).

Figure 2.

Effects of bevacizumab and ranibizumab on the production of nitric oxide in trabecular meshwork cells. Both bev- acizumab and ranibizumab decreased Nitric oxide (NO) production (^*p < 0.05). NO production between bevacizumab and ranibizumab was not different significantly (p = 0.376). VEGF = vascular endothelial growth factor.

Figure 3.

Expression of eNOS mRNA after exposed to bev- acizumab and ranibizumab. β-actin used as internal standard.

Figure 4.

Effects on the expression of eNOS mRNA after exposed to bevacizumab and ranibizumab in trabecular meshwork cells. Both bevacizumab and ranibizumab decreased eNOS expression (^*p < 0.05), and the degree of eNOS expression was not different significantly between bevacizumab and ranibizumab (p = 0.067).

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