Effect of Nitric Oxide on Adhesion and Migration of Trabecular Meshwork Cells

Jin Seong Bae; Jae Woo Kim

doi:10.3341/jkos.2013.54.4.639

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Bae and Kim: Effect of Nitric Oxide on Adhesion and Migration of Trabecular Meshwork Cells

Original Article

J Korean Ophthalmol Soc 2007;54(4):639-644.

Published online: 24 August 2007

DOI: https://doi.org/10.3341/jkos.2013.54.4.639

Effect of Nitric Oxide on Adhesion and Migration of Trabecular Meshwork Cells

Jin Seong Bae, MD, Jae Woo Kim, MD, PhD

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea

Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Daegu Catholic University Medical Center #33 Duryugongwon-ro 17-gil, Nam-gu, Daegu 705-718, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133, E-mail: jwkim@cu.ac.kr

Received 13 July 201219 October 2012 Accepted 21 January 2013

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the effect of nitric oxide (NO) on the adhesion and migration of cultured human trabecular mesh-work cells (HTMC).

Methods

For adhesion assay, primarily cultured HTMC were attached to culture dishes for 1 hr, cells were rinsed, and the remaining adherent cells were assessed with MTT assay. Degree of cellular migration was assessed under normal and stressed conditions using microchemoattraction chambers. Effect of NO on the adhesion and migration was assessed with or without co-exposure of S-nitroso-N-acetylpenicillamine (SNAP).

Results

NO did not affect the degree of adhesion or migration of HTMC (p > 0.05). The degree of adhesion increased although the degree of migration decreased with 1% serum (p < 0.05). Degrees of migrations decreased after mechanical stress (p < 0.05).

Conclusions

NO may not affect the adhesion or migration of HTMC.

J Korean Ophthalmol Soc 2013;54(4):639-644

Keywords: Adhesion, Migration, Nitric oxide, Trabecular meshwork cells

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Figure 1.

Effect of NO (Nitric oxide) donor on the production of NO. S-nitroso-N-acetylpenicillamine (SNAP) increased NO in a dose-dependent manner compared serum-free or 1% fetal bovine serum (FBS) (^*p < 0.05).

Figure 2.

Effect of NO (Nitric oxide) donor on the adhesion of trabecular meshwork cells. S-nitroso-N-acetylpenicillamine (SNAP) did not affect on the cell adhesion, but 1% fetal bovine serum (FBS) increased cellular adhesion significantly (^*p < 0.05).

Figure 3.

Effect of NO (Nitric oxide) donor on the migration of trabecular meshwork cells in normal condition. S-nitrosoN-acetylpenicillamine (SNAP) did not affect the cell migration, but 1% fetal bovine serum (FBS) decreased cellular migration significantly (^*p < 0.05).

Figure 4.

Effect of NO (Nitric oxide) donor on the migration of trabecular meshwork cells in mechanically stressed condition. After stress, the degree of cellular migration decreased in all groups and did not change significantly compared to non-exposed control (p > 0.05).

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