Effect of Dipyridamole on the Reactive Oxygen Species and Oxidative Stress in Trabecular Meshwork Cells

Keun Woo Lee; Jae Woo Kim

doi:10.3341/jkos.2013.54.3.496

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Lee and Kim: Effect of Dipyridamole on the Reactive Oxygen Species and Oxidative Stress in Trabecular Meshwork Cells

Original Article

J Korean Ophthalmol Soc 2007;54(3):496-501.

Published online: 24 August 2007

DOI: https://doi.org/10.3341/jkos.2013.54.3.496

Effect of Dipyridamole on the Reactive Oxygen Species and Oxidative Stress in Trabecular Meshwork Cells

Keun Woo Lee, MD, Jae Woo Kim, MD, PhD

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea

Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Daegu Catholic University Medical Center #33 17-gil Duryugongwon-ro, Nam-gu, Daegu 705-718, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133, E-mail: jwkim@cu.ac.kr

Received 13 July 201211 October 2012 Accepted 14 February 2013

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the effects of dipyridamole (DPD) on the production of reactive oxygen species (ROS) and oxidative stress in cultured human trabecular meshwork cells (HTMC).

Methods

Antioxidant activity of DPD was determined by DPPH assay. Primarily cultured HTMC were exposed to 0, 20, and 50 μm DPD using serum-deprived media. The effect of DPD on the production of ROS was assessed with the DCHFDA assay. The effect of DPD on the t-butyl hydroperoxide (tBHP)-induced oxidative stress was assessed with resazurin assay.

Results

DPD showed significant antioxidant activity. DPD significantly decreased the production of ROS (p < 0.05) and improved cellular activity significantly after treatment with t-BHP (p < 0.05). DPD did not affect the generation of nitric oxides.

Conclusions

DPD suppressed the formation of ROS and possessed cytoprotective activity against the oxidative stress in HTMC.

Keywords: Antioxidant, Dipyridamole, Reactive oxygen species, Trabecular meshwork cells

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Figure 1.

Antioxidant property of dipyridamole with DPPH assay. Dipyridamole reduced DPPH in a dose-dependent manner (^*p < 0.05).

Figure 2.

Effect of dipyridamole on the generation of reactive oxygen species (ROS) in trabecular meshwork cells with DCFHDA assay. Dipyridamole decreased ROS significantly in a dose-dependent manner (^*p < 0.05).

Figure 3.

Effect of dipyridamole on the tBHP-induced oxidative stress in trabecular meshwork cells with resazurin assay. Exposure to tBHP decreased cellular activity significantly compared to non-exposed control (^*p < 0.05). Dipyridamole improved cellular activity significantly after treatment with tBHP (^**p < 0.05).

Figure 4.

Effect of dipyridamole on the production of nitric oxide in trabecular meshwork cells with Griess assay. Dipyridamole did not affect the production of nitric oxide significantly (p > 0.05).

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