Journal List > J Korean Ophthalmol Soc > v.54(3) > 1009627

Lee and Kim: Effect of Dipyridamole on the Reactive Oxygen Species and Oxidative Stress in Trabecular Meshwork Cells

Abstract

Purpose

To investigate the effects of dipyridamole (DPD) on the production of reactive oxygen species (ROS) and oxidative stress in cultured human trabecular meshwork cells (HTMC).

Methods

Antioxidant activity of DPD was determined by DPPH assay. Primarily cultured HTMC were exposed to 0, 20, and 50 μm DPD using serum-deprived media. The effect of DPD on the production of ROS was assessed with the DCHFDA assay. The effect of DPD on the t-butyl hydroperoxide (tBHP)-induced oxidative stress was assessed with resazurin assay.

Results

DPD showed significant antioxidant activity. DPD significantly decreased the production of ROS (p < 0.05) and improved cellular activity significantly after treatment with t-BHP (p < 0.05). DPD did not affect the generation of nitric oxides.

Conclusions

DPD suppressed the formation of ROS and possessed cytoprotective activity against the oxidative stress in HTMC.

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Figure 1.
Antioxidant property of dipyridamole with DPPH assay. Dipyridamole reduced DPPH in a dose-dependent manner (*p < 0.05).
jkos-54-496f1.tif
Figure 2.
Effect of dipyridamole on the generation of reactive oxygen species (ROS) in trabecular meshwork cells with DCFHDA assay. Dipyridamole decreased ROS significantly in a dose-dependent manner (*p < 0.05).
jkos-54-496f2.tif
Figure 3.
Effect of dipyridamole on the tBHP-induced oxidative stress in trabecular meshwork cells with resazurin assay. Exposure to tBHP decreased cellular activity significantly compared to non-exposed control (*p < 0.05). Dipyridamole improved cellular activity significantly after treatment with tBHP (**p < 0.05).
jkos-54-496f3.tif
Figure 4.
Effect of dipyridamole on the production of nitric oxide in trabecular meshwork cells with Griess assay. Dipyridamole did not affect the production of nitric oxide significantly (p > 0.05).
jkos-54-496f4.tif
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