The Wound Healing Effects of AmniSite-Lens in Rabbits

Tae Hyung Kim; Yeoun Sook Chun; Jae Chan Kim

doi:10.3341/jkos.2013.54.2.338

Journal List > J Korean Ophthalmol Soc > v.54(2) > 1009600

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Kim, Chun, and Kim: The Wound Healing Effects of AmniSite-Lens in Rabbits

Original Article

J Korean Ophthalmol Soc 2007;54(2):338-345.

Published online: 24 August 2007

DOI: https://doi.org/10.3341/jkos.2013.54.2.338

The Wound Healing Effects of AmniSite-Lens in Rabbits

Tae Hyung Kim, MD, Yeoun Sook Chun, MD, PhD, Jae Chan Kim, MD, PhD

Department of Ophthalmology, Chung-Ang University College of Medicine, Seoul, Korea

Address reprint requests to Jae Chan Kim, MD, PhD, Department of Ophthalmology, Chung-Ang University Hospital, #102 Heukseok-ro, Dongjak-gu, Seoul 156-755, Korea Tel: 82-2-6299-1665, Fax: 82-2-825-1666, E-mail: jck50ey@kornet.net

Received 8 September 201220 October 2012 Accepted 23 January 2013

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

The effects of AmniSite-Lens on wound healing were evaluated for a burn wound on a rabbit cornea.

Methods

A chemical burn was inflicted on the cornea of rabbits using 0.1N NaOH and a superficial keratectomy with trephine was performed. The control group consisted of rabbits with a bandage contact lens (Focus Lens) after the operation. In the other group, the AmniSite-Le was applied on the rabbits' cornea. The rabbits were evaluated for the following: 1) the time of epithelialization; 2) the grade of corneal opacity; and 3) the histological analysis by evaluation of inflammatory reaction and apoptotic keratocytes.

Results

In the alkali-burn model, the time of epithelialization in the AmniSite-Lens group was not statistically significant compared with the bandage contact lens group. There was no difference of corneal opacity at postoperative week 1. The corneal opacity in the AmniSite-Lens group was clearer than the bandage contact lens group at postoperative weeks 4 and 8 and the difference of corneal opacity was statistically significant. In the keratectomy model, the time of epithelialization in the AmniSite-Lens group was not statistically significant compared with the bandage contact lens group. The corneal opacity in the AmniSite-Lens group was clearer than the bandage contact lens group at postoperative weeks 1 and 4 and the difference of corneal opacity was statistically significant.

Conclusions

The AmniSite-Lens has both the wound healing effect of an amniotic membrane and the convenience of a bandage contact lens.

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Keywords: Amniotic membrane, Amniotic membrane contact lens, AmniSite-Lens, Chemical burn, Wound healing

References

1. Shimmura S, Shimazaki J, Ohashi Y, Tsubota K. Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders. Cornea. 2001; 20:408–13.

2. Kim JS, Kim JC, Na BK, et al. Amniotic membrane patching promotes healing and inhibits proteinase activity on wound healing following acute corneal alkali burn. Exp Eye Res. 2000; 70:329–37.

3. Gris O, del Campo Z, Wolley-Dod C, et al. Amniotic membrane implantation as a therapeutic contact lens for the treatment of epithelial disorders. Cornea. 2002; 21:22–7.

4. Takano Y, Fukagawa K, Miyake-Kashima M, et al. Dramatic healing of an allergic corneal ulcer persistent for 6 months by amniotic membrane patching in a patient with atopic keratoconjunctivitis: a case report. Cornea. 2004; 23:723–5.

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Figure 1.

The structure of amniotic membrane contact lens.

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Figure 2.

Photographs showing corneal opacity at 1 day (A, B), 1 week (C, D), and 4 weeks (E, F) after superficial keratectomy. Corneal opacity was improved in all groups, but in the bandage contact lens group (B, D, F), the opacity was more prominent than in AmniSite-Lens group (A, C, E). The difference of cornea opacity was statistically significant at 1 week and 4 weeks (*p < 0.05).

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Figure 3.

Photographs showing corneal opacity at 1 day (A, B), 1 week (C, D), 4 weeks (E, F), and 8 weeks (G, H) after alkali burn. Corneal opacity was improved in all groups, but in the bandage contact lens group (B, D, G, H), the opacity was more prominent than in AmniSite-Lens group (A, C, E, G). The difference of cornea opacity was statistically significant at 4, 8 weeks (*p < 0.05).

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Figure 4.

Histopathologic findings of rabbit cornea with hematoxylin and eosin staining (×200). M ild infiltration of inflammatory cells was detected in the AmniSite-Lens (A) and bandage contact lens group (B) of the alkali-burn model and the AmniSite-Lens (C) and bandage contact lens group (D) of the superficial keratectomy model.

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Figure 5.

Comparison of PMNs infiltration in the stroma. There was significant difference between the AmniSite-Lens and bandage CL group (*p < 0.05). (A) Alkali burn model, (B) Superficial keratectomy model. PM Ns = polymorphonuclear cells; HPF = high power field.

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Figure 6.

Histopathologic findings of the rabbit cornea with TUNEL stain (×400). The lesser TUNEL positive cells were detected in the AmniSite-Lens (A) than in the bandage contact lens group (B) of the alkali-burn model. Also, lesser TUNEL positive cells were detected in the AmniSite-Lens (C) than in the bandage contact lens group (D) of the superficial keratectomy model.

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Figure 7.

Comparison of TUNEL positive cell counts. More TUNEL positive cells were seen in the bandage contact lens group. There was significant difference between the AmniSite-Lens and the bandage CL group (*p < 0.05). (A) Alkali burn model, (B) Superficial keratectomy model. HPF = high power field.

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