Effect of Curcumin in a Mouse Model of Oxygen-Induced Retinopathy

Sang Jin Kim; Young Ri Gu; Hyeong Gon Yu

doi:10.3341/jkos.2013.54.10.1588

Journal List > J Korean Ophthalmol Soc > v.54(10) > 1009512

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Kim, Gu, and Yu: Effect of Curcumin in a Mouse Model of Oxygen-Induced Retinopathy

Original Article

J Korean Ophthalmol Soc 2013;54(10):1588-1593.

Published online: 3 September 2013

DOI: https://doi.org/10.3341/jkos.2013.54.10.1588

Effect of Curcumin in a Mouse Model of Oxygen-Induced Retinopathy

Sang Jin Kim, MD^1,^2,³, Young Ri Gu, MD², Hyeong Gon Yu, MD^1,²

¹Departments of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea

²Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea

³Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

Address reprint requests to Hyeong Gon Yu, MD, PhD, Department of Ophthalmology, Seoul National University Hospital, #101 Daehak-ro, Jongno-gu, Seoul 110-744, Korea, Tel: 82-2-2072-2438, Fax: 82-2-741-3187, E-mail: hgonyu@snu.ac.kr

Received 17 November 2012 Revised 28 January 2013 Accepted 24 August 2013

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the effect of curcumin, known to inhibit hypoxia-inducible factor-1, on retinal neovascularization in a mouse model of oxygen-induced retinopathy (OIR).

Methods

OIR was induced by exposing C57BL/6 mice on postnatal day 7 (P7) to 75% hyperoxia for 5 days, followed by 5 days in a room with normal oxygen level. Curcumin was administered intraperitoneally once a day for 5 days from P12 or intravitreally once on P13. Mice retinas on P17 were analyzed for retinal neovascularization, which was compared between curcumin-treated and control mice.

Results

After intraperitoneal and intravitreal administration of curcumin, qualitative assessment of retinal neovascularization of flat-mounted retina showed no significant difference compared to control retinas. Quantitative assessment of retinal neovascularization also showed no significant difference between curcumin-treated and control mice.

Conclusions

Both intraperitoneal and intravitreal administration of curcumin did not reduce retinal neovascularization in an OIR mouse model. Further investigation including development of new formulations is required for the use of curcumin as an anti-angiogenic agent for retinal neovascularization.

Keywords: Angiogenesis, Curcumin, Oxygen-induced retinopathy, Retinopathy of Prematurity

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Figure 1.

Fluorescence micrographs of flat-mounted retinas from the mouse model of oxygen-induced retinopathy (OIR). (A) Control OIR retina treated with intraperitoneal PBS injection. (B) OIR retina treated with intraperitoneal curcumin (100 mg/kg) injection. (C) Control OIR retina treated with intravitreal PBS injection. (D) OIR retina treated with intra- vitreal curcumin (1 μg) injection.

Figure 2.

Quantitative assessment of retinal neovascularization in the mouse model of oxygen-induced retinopathy treated with intraperitoneal curcumin injection. (A) The average vascular lumens per section for each group are presented as the means ± SEM. Note that there is no significant difference in the retinal neovascularization between the control and curcu- min-treated eyes. Representative photomicrographs of OIR retinas treated with intraperitoneal PBS (B) and intraperitoneal curcumin (100 mg/kg) injection (C).

Figure 3.

Quantitative assessment of retinal neovascularization in the mouse model of oxygen-induced retinopathy treated with intravitreal curcumin injection. (A) The average vascular lumens per section for each group are presented as the means ± SEM. Note that there is no significant difference in the retinal neovascularization between the control and curcumin treated eyes. Representative photomicrographs of OIR retinas treated with intravitreal PBS (B) and intraperitoneal curcumin (1 μg) injection (C).

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