Inflammatory Cytokines in the Vitreous of Rabbits after Photocoagulation Using Pattern Scanning and Conventional Laser

Young Rock Choi; Ik Soo Byon; Seung Uk Lee; Min Kyu Shin; Ji Eun Lee; Boo Sup Oum

doi:10.3341/jkos.2013.54.1.149

Journal List > J Korean Ophthalmol Soc > v.54(1) > 1009496

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Choi, Byon, Lee, Shin, Lee, and Oum: Inflammatory Cytokines in the Vitreous of Rabbits after Photocoagulation Using Pattern Scanning and Conventional Laser

Original Article

J Korean Ophthalmol Soc 2013;54(1):149-154.

Published online: 25 September 2013

DOI: https://doi.org/10.3341/jkos.2013.54.1.149

Inflammatory Cytokines in the Vitreous of Rabbits after Photocoagulation Using Pattern Scanning and Conventional Laser

Young Rock Choi, MD, PhD¹, Ik Soo Byon, MD^2,³, Seung Uk Lee, MD⁴, Min Kyu Shin, MD², Ji Eun Lee, MD, PhD^2,³, Boo Sup Oum, MD, PhD^2,^3,

¹Daeyoun Saint Mary Eye Clinic, Busan, Korea

²Department of Ophthalmology, Pusan National University School of Medicine, Busan, Korea

³BiomMedical Instutute, Pusan National University Hospital, Busan, Korea

⁴Department of Ophthalmology, Kosin University College of Medicine, Busan, Korea

Address reprint requests to Boo Sup Oum, MD, PhD Department of Ophthalmology, Pusan National University Hospital #179 Gudeok-ro, Seo-gu, Busan 602-739, Korea Tel: 82-51-240-7321, Fax: 82-51-242-7341, E-mail: bsoum@pusan.ac.kr

Received 31 May 2011 Accepted 14 September 2012

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To compare concentration of cytokines in the vitreous of rabbit eyes after photocoagulation using a short-pulse pattern scanning laser (PASCAL) or a conventional laser.

Methods

Laser photocoagulation was performed using PASCAL (duration 0.02 seconds) in the right eyes and a conventional laser (duration 0.1 seconds) in the left eyes of 13 pigmented rabbits. To obtain ophthalmoscopically similar mild burns, power was adjusted during the photocoagulation. The rabbits were sacrificed at 1, 3 or 7 days after photocoagulation to investigate histological changes. Levels of interleukins (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α in the vitreous humors of ten rabbits were measured using enzyme-linked immunosorbent assay before treatment and at 1, 3 and 7 days after photocoagulation.

Results

Histological changes were comparable between the two groups. IL-1β and TNF-α were not detectable. IL-6 did not change significantly. IL-8 increased at day 3 and day 7, but no significant difference was observed between the two groups (p＞0.05).

Conclusions

When ophthalmoscopically similar mild burn intensity was achieved, conventional and PASCAL treatments of rabbit eyes caused no difference in intravitreal concentration of inflammatory cytokines.

Keywords: Cytokine, Interleukin, Patterned scanning laser, Photocoagulation

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Figure 1.

Ophthalmoscopic findings after photocoagulation using pattern scanning laser (A: day 0; C: day 1; E: day 3; G: day 7) and conventional laser (B: day 0; D: day 1; F: day 3; H: day 7).

Figure 2.

Microscopic findings after photocoagulation using pattern scanning laser (A: day 1; C: day 3; E: day 7) and conventional laser (B: day 1; D: day 3; F: day 7).

Figure 3.

Intravitreal concentration of interleukin-6 (IL-6) after photocoagulation using pattern scanning laser and conventional laser. Each symbol represents the lowest, the average and the highest concentration.

Figure 4.

Intravitreal concentration of interleukin-8 (IL-8) after photocoagulation using pattern scanning laser and conventional laser (^* p<0.05, compared to baseline). Each symbol represents the lowest, the average and the highest concentration.

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