Young Jin Lim; Gyu Nam Kim; Na Eun Lee; Sung Jae Kim; Ji Myong Yoo

doi:10.3341/jkos.2013.54.1.143

Journal List > J Korean Ophthalmol Soc > v.54(1) > 1009485

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Lim, Kim, Lee, Kim, and Yoo: Decorin and TGF-β Expression after Partial Myotomy of the Extraocular Muscle in Rat

Original Article

J Korean Ophthalmol Soc 2013;54(1):143-148.

Published online: 25 September 2013

DOI: https://doi.org/10.3341/jkos.2013.54.1.143

Decorin and TGF-β Expression after Partial Myotomy of the Extraocular Muscle in Rat

Young Jin Lim, MD¹, Gyu Nam Kim, MD¹, Na Eun Lee, MD¹, Sung Jae Kim, MD¹, Ji Myong Yoo, MD, PhD^1,^2,

¹Department of Ophthalmology, Gyeongsang National University School of Medicine, Jinju, Korea

²Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, Korea

Address reprint requests to Ji Myong Yoo, MD, PhD Department of Ophthalmology, Gyeongsang National University Hospital #79 Gangnam-ro, Jinju 660-702, Korea Tel: 82-55-750-8164, Fax: 82-55-758-4158, E-mail: YJM@gnu.ac.kr

Received 11 July 2011 Accepted 30 June 2012

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To report the expression of decorin and TGF-β in partial myotomy of the extraocular muscle in rats.

Methods

Partial myotomy of the superior rectus muscle was performed on the right eye of 10 Sprague-Dawley rats followed by exposure of the left superior rectus muscle and a simple suture of the conjunctiva. The bilateral superior rectus muscle was obtained from all rats at 2 weeks postoperatively. The tissues were observed under light microscopy with hem-atoxylin-eosin, Masson's trichrome staining and immunohistochemistry.

Results

Histological examinations of the surgical area at 2 weeks after postoperatively showed irregularly concentrated fibrosis on light microscopy with hematoxylin-eosin and Masson's trichrome staining of the experimental eyes. Immnohistochemistry showed that expression of decorin was in the same location as TGF-β in the experimental group.

Conclusions

The expression of decorin was found in the healing process after partial myotomy of the extraocular muscle in rats. Immunohistochemistry showed that expression of decorin was in the same location as with TGF-β.

Keywords: Decorin, Extraocular muscle, Fibrosis, Tumor growth factor

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Figure 1.

Light microscopic findings of the superior rectus muscles at 2 weeks after operation (H&E stain). (A, ×40; B, ×100) Control group. (C, ×40; D, ×100) Experimental group. The increasing density of fibrosis is observed in the experimental groups compared with control groups. SR = superior rectus.

Figure 2.

Light microscopic findings of the superior rectus muscles at 2 weeks after operation (Masson's trichrome stain). (A, ×40; B, ×100) Control group. (C, ×40; D, ×100) Experimental group. The irregularity of fibrotic bands is observed more than in the experimental groups compared with control groups. SR = superior rectus.

Figure 3.

The immunohistochemical staining of decorin (A, D ×200), TGF-β (B, E ×200) and merging (C, F ×200) at 2 weeks after operation. (A, B, C) No definite immune activity decorin or TGF-β were found. (D, E) The immune activity of decorin and TGF-β is observed. (F) Decorin and TGF-β are expressed at the same sites.

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