Sun Hee Kang; Jae Woo Kim

doi:10.3341/jkos.2013.54.9.1423

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Kang and Kim: Effects of Prednisolone and Loteprednol Eyedrops on the Proliferation of Human Tenon’s Capsule Fibroblasts

Original Article

J Korean Ophthalmol Soc 2013;54(9):1423-1428.

Published online: 7 September 2013

DOI: https://doi.org/10.3341/jkos.2013.54.9.1423

Effects of Prednisolone and Loteprednol Eyedrops on the Proliferation of Human Tenon’s Capsule Fibroblasts

Sun Hee Kang, MD, Jae Woo Kim, MD, PhD

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea

Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Daegu Catholic University Medical Center, #33 Duryugongwon-ro 17-gil, Nam-gu, Daegu 705-718, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133 E-mail: jwkim@cu.ac.kr

Received 5 April 20132 May 2013 Accepted 10 August 2013

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To compare the effect of loteprednol etabonate (LE) with prednisolone acetate (PDA) drops on the proliferation of human Tenon’s capsule fibroblasts (HTFBs).

Methods

Primarily cultured HTFBs were treated with serially diluted PDA and LE for 3 days. Cellular survival was de-termined by a rapid colorimetric assay using MTT. RT-PCR was performed to determine the relative expression of TGF-β mRNA in response to LE and PDA.

Results

PDA inhibited proliferation of HTCF in a dose-dependent manner and LE inhibited significantly the proliferation of HTCF at the higher concentration of 50 μ g/ml (p < 0.05). Compared to LE, PDA inhibited proliferation of HTCF significantly at each diluted concentration (p < 0.05). Expressions of TGF-β were decreased as the concentration of both PDA and LE increased. PDA decreased expression of TGF-β more significantly compared to LE at each concentration (p < 0.05).

Conclusions

Although LE has offered promising anti-inflammatory efficacy with decreased impact on intraocular pressure, LE may be less effective than PDA in inhibiting fibroblast proliferation and may be not comparable to PDA in preventing ex-cessive scarring after glaucoma filtering surgery.

Keywords: Fibroblasts, Loteprednol etabonate, Prednisolone acetate, Proliferation, TGF-β

References

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Figure 1.

Effect of 1% prednisolone acetate on the proliferation of human Tenon's capsule fibroblasts in tissue culture. Prednisolone acetate inhibited significantly the proliferation of fibroblasts compared to non-exposed controls at each concentration: ^* p < 0.05.

Figure 2.

Effect of 0.5% loteprednol etabonate on the pro-liferation of human Tenon's capsule fibroblasts in tissue culture. Loteprednol etabonate inhibited significantly the pro-liferation of fibroblasts only at concentration of 50 μ g/ml com-pared to non-exposed controls: ^* p < 0.05.

Figure 3.

Comparison of the effects of topical steroids on the proliferation of human Tenon's capsule fibroblasts in tissue culture. Prednisolone acetate (PDA) inhibited significantly the proliferation of fibroblasts more compared to loteprednol eta-bonate (LE) at each concentration: ^* p < 0.05.

Figure 4.

Effect of topical steroids on the expression of TGF-β mRNA in human Tenon's capsule fibroblasts. Prednisolone acetate (PDA) inhibited more significantly the expression of TGF-β mRNA compared to loteprednol etabonate (LE) at each concentration: ^* p < 0.05.

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