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Kong, Cho, and Kim: Analysis of Factors Affecting the Decrease of Endothelial Cell Density in Imported Donor Corneas
Original Article
Journal of the Korean Ophthalmological Society

Journal of the Korean Ophthalmological Society 2012; 53(1): 20-26.

Published online: 16 January 2012

DOI: https://doi.org/10.3341/jkos.2012.53.1.20

Analysis of Factors Affecting the Decrease of Endothelial Cell Density in Imported Donor Corneas

Seok Joon Kong, MD1, Kyongjin Cho, MD2, Man Soo Kim, MD, PhD1

1Department of Ophthalmology and Visual Science, College of Medicine, The Catholic University of Korea, Seoul, Korea.

2Department of Ophthalmology, The Dankook University Medical College, Cheonan, Korea.

Address reprint requests to Man Soo Kim, MD, PhD. Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital, The Catholic University of Korea, #222 Banpo-daero, Seocho-gu, Seoul 137-701, Korea. Tel: 82-2-2258-6197, Fax: 82-2-599-7405, mskim@catholic.ac.kr

Received 25 April 2011       Accepted 23 November 2011

Copyright © 2012 The Korean Ophthalmological Society

Abstract

Purpose

To evaluate the difference between corneal endothelial cell density at the moment of preservation and at keratoplasty in imported donor corneas and to analyze the correlated factors of the difference.

Methods

Eighty-seven imported corneas were evaluated. Corneal endothelial cell density at the moment of preservation was obtained from the medical record and was measured just before the keratoplasty. Correlation of the difference in endothelial cell density with the following factors were analyzed; donor sex, donor age, death-to-preservation time, preservation-to-surgery time, death-to-surgery time, endothelial cell density at the moment of preservation, and preservation period of the corneas.

Results

All of the corneas showed a decrease in endothelial cell density. Mean endothelial cell density of imported donor corneas at the moment of preservation and at keratoplasty was 2789 ± 235 cells/mm2 and 2592 ± 254 cells/mm2 (p < 0.001), respectively. Mean endothelial cell loss was 197 ± 148 cells/mm2, which was significantly correlated with preservation-to-surgery time, death-to-surgery time and a preservation period longer than 7 days (p = 0.042, p = 0.045, p = 0.036, respectively).

Conclusions

Reduced death-to-surgery time and keratoplasty before 7 days of preservation are needed for better surgical outcome.

Keywords: Death to surgery time, Endothelial cell density, Imported donor corneas

Figures and Tables

Figure 1
Mean endothelial cell loss count of eye bank.
jkos-53-20-g001
Figure 2
Correlation between donor age and endothelial cell loss count (r = -0.049, p = 0.650).
jkos-53-20-g002
Figure 3
Correlation between death to preservation time and endothelial cell loss count (r = -0.040, p = 0.714). Mean death to preservation time was 9.0 ± 5.5 hours.
jkos-53-20-g003
Figure 4
Correlation between death to surgery time and endothelial cell loss count (r = 0.216, p = 0.045). Mean death to surgery time was 152.2 ± 53.0 hours (6.34 days).
jkos-53-20-g004
Table 1
Age and sex distribution of imported donor corneas
jkos-53-20-i001
Table 2
Causes of death in imported donor corneas
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Table 3
Endothelial cell changes and time distribution of imported donor cornea
jkos-53-20-i003

Values are mean ± SD.

Table 4
Changes in number of eyes of endothelial cell density
jkos-53-20-i004
Table 5
Number of eyes of endothelial cell loss count
jkos-53-20-i005
Table 6
Demographic features of the sex of imported donor cornea
jkos-53-20-i006

Values are mean ± SD.

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