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Lee and Ahn: Human Vitreous Proteomes in Idiopathic Macular Hole
Original Article
Journal of the Korean Ophthalmological Society

Journal of the Korean Ophthalmological Society 2012; 53(10): 1512-1522.

Published online: 17 October 2012

DOI: https://doi.org/10.3341/jkos.2012.53.10.1512

Human Vitreous Proteomes in Idiopathic Macular Hole

Kwang Hoon Lee, MD1, Jae Kyoun Ahn, MD, PhD2

1Happyeye21 Clinic, Gwangju, Korea.

2Samsung Eye & ENT Clinic Center, Jeonju, Korea.

Address reprint requests to Jae Kyoun Ahn, MD, PhD. Samsung Eye & ENT Clinic Center, #225 Ujeon-ro, Wansan-gu, Jeonju 560-800, Korea. Tel: 82-63-220-0114, Fax: 82-63-220-0100, Opth20@freechal.com

Received 17 February 2012       Accepted 25 August 2012

Copyright © 2012 The Korean Ophthalmological Society

Abstract

Purpose

To investigate the vitreous proteomic profiles of macular hole (MH) patients compared to donor eyes and epiretinal membrane (ERM) patients.

Methods

Ten donor eyes, 25 MH patients (15 MH without posterior vitreous detachment (PVD) and 10 MH with PVD), 10 ERM patients and 4 patients with vitreomacular traction syndrome (VMTS) were enrolled in the present study. The undiluted vitreous samples were obtained from all eyes. Proteins were identified using the micro-RPLC/MS/MS technique and using mass spectrometric data analysis from the IPI database. Proteins were searched for the function, subcellular location and tissue specificity using the Uniprot database.

Results

The mean vitreous protein concentrations were the highest (p < 0.01) in MH patients without PVD. There were no qualitative changes according to the age of donor eyes. However, the authors newly-identified 12 proteins (i.e. vasorin) in donor eyes. Ten out of 136 proteins (7.3%) (i.e. retinoschisin) were exclusively detected in the MH samples. S100A8 was exclusively detected in eyes without PVD, whereas CD59 was exclusively detected in eyes with PVD.

Conclusions

The results suggest there is a small difference in the vitreous proteomic profiles in MH patients compared to donor eyes or ERM patients, and that the expression of vitreous proteins is closely associated with the presence of PVD.

Keywords: Macular hole, Posterior vitreous detachment, Proteomics, S100, Vitreous

Figures and Tables

Figure 1
Classification of patients based on the features of optical coherence tomography (OCT). VMTS = vitreomacular traction syndrome; MH = macular hole; PVD = posterior vitreous detachment; ERM = epiretinal membrane.
jkos-53-1512-g001
Figure 2
Comparison of vitreous proteomes among donor eyes, macular hole (MH) samples and epiretinal membrane (ERM) samples. (A) Average protein concentrations in vitreous. (B) Venn diagram of proteins identified from the control (donor eye), MH and ERM samples. (C) Function categorizations of vitreous proteins identified.
jkos-53-1512-g002
Figure 3
Comparison of vitreous proteomes according to the presence of posterior vitreous detachment (PVD). (A) Average protein concentrations in vitreous. (B) Venn diagram of proteins identified based on the status of PVD. (C) Function categorizations of vitreous proteins identified.
jkos-53-1512-g003
Figure 4
Representative MS/MS spectrum from four proteins that was identified specific for macular hole and epiretinal membrane.
jkos-53-1512-g004
Table 1
Demographic features of enrolled patients
jkos-53-1512-i001

Values are presented as mean ± SD or number.

VMTS = vitreomacular traction syndrome; MH = macular hole; PVD = posterior vitreous detachment; ERM = epiretinal membrane.

Table 2
Comparison of vitreous proteomes in donor eyes according to the ages
jkos-53-1512-i002

Values are presented as mean ± SD unless otherwise indicated.

IC = intracellular; EC = extracellular; Mem = cell membrane.

Table 3
Vitreous proteins newly identified in donor eyes by micro-RPLC-MS/MS
jkos-53-1512-i003

SC = spectrum counts; Mem = cell membrane; CNS = central nervous system; EC = extracellular; NA = not applicable.

Table 4
Different profiles of vitreous proteins identified by micro-RPLC-LC-MS/MS in donor eye, epiretinal membrane and macular hole samples
jkos-53-1512-i004

SC = spectrum counts; EC = extracellular; NA = not applicable; IC = intracellular; CNS = central nervous system.

Table 5
Different profiles of vitreous proteins identified by micro-RPLC-LC-MS/MS according to the presence of posterior vitreous detachment
jkos-53-1512-i005

SC = spectrum counts; EC = extracellular; IC = intracellular; NA = not applicable; CNS = central nervous system.

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