Effect of Genistein on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells

Jung Heum Hong; Yun Young Kim; Jae Woo Kim

doi:10.3341/jkos.2011.52.8.970

Journal List > J Korean Ophthalmol Soc > v.52(8) > 1009107

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Hong, Kim, and Kim: Effect of Genistein on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells

Original Article

J Korean Ophthalmol Soc 2011;52(8):970-974.

Published online: 7 September 2011

DOI: https://doi.org/10.3341/jkos.2011.52.8.970

Effect of Genistein on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells

Jung Heum Hong, MD, Yun Young Kim, MD, PhD, Jae Woo Kim, MD, PhD

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea

Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Daegu Catholic University Medical Center #3056-6 Daemyeong 4-dong, Nam-gu, Daegu 705-718, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133, E-mail: jwkim@cu.ac.kr

Received 10 February 2011 Revised 15 March 2011 Accepted 31 May 2011

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the effects of genistein on the survival and production of nitric oxide in cultured human trabecular meshwork cells (HTMC).

Methods

Primarily cultured HTMC were exposed to 0, 1, and 10 μ m genistein using serum-deprived media. Production of nitric oxide and eNOS activity were assessed with the Griess assay and RT-PCR after exposure to genistein for 10 min and one day. Cellular survival was assessed via MTT assay after exposure to genistein for one day.

Results

Genistein significantly increased the production of nitric oxide after exposure for 1 min at 10 μ m and for 1 day at 1 μ m under serum-deprived conditions. Genistein increased eNOS activity and cellular survival in HTMC.

Conclusions

Genistein increases cellular survival under serum-deprived conditions, accompanied with an increase in nitric oxide production after both short-term and long-term exposures.

Keywords: Genistein, Nitric oxide, Survival, Trabecular meshwork cells

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Figure 1.

Effect of genistein on the production of nitric oxide in trabecular meshwork cells after exposure for 10 minutes (^* p < 0.05).

Figure 2.

Effect of genistein on the production of nitric oxide in trabecular meshwork cells after exposure for 1 day (^*p < 0.05).

Figure 3.

Effect of genistein on the activity of eNOS. Genistein activated eNOS after 10 min (A) and 1 day exposures (B). Lane 1, 5 for control, lane 2, 6 for 0.1 μ m, lane, 3, 7: for 0.1 μ m + L-NAME, lane 4, 8 for 1 μ m.

Figure 4.

Effect of genistein on the survival of trabecular meshwork cells after exposure for 1 days (^* p < 0.05).

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