Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells

Seung Hee Lee; Jae Woo Kim

doi:10.3341/jkos.2011.52.12.1514

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Lee and Kim: Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells

Original Article

J Korean Ophthalmol Soc 2011;52(12):1514-1518.

Published online: 7 September 2011

DOI: https://doi.org/10.3341/jkos.2011.52.12.1514

Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells

Seung Hee Lee, MD, Jae Woo Kim, MD, PhD

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea

Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Daegu Catholic University Medical Center #3056-6 Daemyeong 4-dong, Nam-gu, Daegu 705-718, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133, E-mail: jwkim@cu.ac.kr

Received 6 April 201110 May 2011 Accepted 30 September 2011

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the effects of erythropoietin (EPO) on the production of nitric oxide in cultured human trabecular meshwork cells (HTMC).

Methods

Primarily cultured HTMC were exposed to 0, 0.5, and 1.0 U/ml EPO using serum-deprived media for 2 days. Production of nitric oxide and cellular survival were assessed with Griess assay and MTT assay, respectively. Expression of EPO mRNA receptor and activity of eNOS were assessed with RT-PCR.

Results

EPO did not affect the survival of HTMC (p > 0.05). EPO increased the production of nitric oxide (p < 0.05) accom-panied with increased eNOS activity.

Conclusions

EPO had no effect on the cellular survival under serum-deprived conditions. EPO increases the production of nitric oxide by increasing eNOS activity.

Keywords: Erythropoietin, Nitric oxide, Trabecular meshwork cells

References

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Figure 1.

Effects of erythropoietin on the survival of trabecular meshwork cells. Erythropoietin did not affect cellular survival (p > 0.05).

Figure 2.

Effects of erythropoietin on the production of nitric oxide in trabecular meshwork cells. Erythropoietin increased NO production (^∗ p < 0.05).

Figure 3.

Expression of erythropoietin mRNA receptor in trabecular meshwork cells. Exposure of 0.5 U/ml erythropoietin (right) increased expression of erythropoietin mRNA receptor compare to the non-exposed control (left).

Figure 4.

Effects of erythropoietin on the activity of eNOS in trabecular meshwork cells. Exposure to 0.5, 1.0 U/ml erythropoietin increased eNOS expression up to 44.5% and 41.2%, respectively compared to the non-exposed control.

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