Effects of Mitomycin C on Eyelash-Regrowth in Epilated Rats

Woong Sun Yoo; Che Ron Kim; Kyeong Hyun Kim; Seong Wook Seo

doi:10.3341/jkos.2011.52.12.1501

Journal List > J Korean Ophthalmol Soc > v.52(12) > 1008958

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Yoo, Kim, Kim, and Seo: Effects of Mitomycin C on Eyelash-Regrowth in Epilated Rats

Original Article

J Korean Ophthalmol Soc 2011;52(12):1501-1506.

Published online: 7 September 2011

DOI: https://doi.org/10.3341/jkos.2011.52.12.1501

Effects of Mitomycin C on Eyelash-Regrowth in Epilated Rats

Woong Sun Yoo, MD¹, Che Ron Kim, MD¹, Kyeong Hyun Kim, MD¹, Seong Wook Seo, MD, PhD^1,²

¹Department of Ophthalmology, School of Medicine, Gyeongsang National University, Jinju, Korea

²Gyeongsang Institute of Health Science, Gyeongsang National University, Jinju, Korea

Address reprint requests to Seong Wook Seo, MD, PhD Department of Ophthalmology, Gyeongsang National University Hospital #79 Gangnam-ro, Jinju 660-702, Korea Tel: 82-55-750-8171, Fax: 82-55-753-4158, E-mail: stramast@naver.com

Received 2 May 201125 May 2011 Accepted 18 October 2011

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

The aim of the present study was to determine the effects of mitomycin C (MMC) on eyelash regrowth in rats.

Methods

Ten rats were subjected to bilateral eyelid epilation. One drop of 0.04% MMC solution and one drop of normal saline was then administered to the right and left eyelid, respectively. Three months post-epliation, the number of regrown eyelashes was counted, and a section of tissue from each eyelid was examined under light- and electron microscopies.

Results

The number of regrown long eyelashes was 1.0 ± 0.81 on the right, and 12.5 ± 1.08 on the left. The rate of regrowth was 7.35 ± 5.55% on the right and 98.45 ± 3.27% on the left. Histological examination revealed no significant differences between the right and left eyelids, except for the absence of any hair shafts in the follicles on the right. However, electron microscopy revealed mitochondrial damage in the follicles of the right eyelid. These changes were not observed in tissue from the left eyelid.

Conclusions

The application of 0.04% MMC inhibited the regrowth of normal eyelashes in rats following simple epilation. These results suggest that MMC may be a novel therapy for replacing invasive surgery in trichiasis with no malpositioning.

Keywords: Eyelash, Mitomycin C, Trichiasis

References

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Figure 1.

Photographs of the eyes of an experimental rat. Long eyelashes of the left (A, B) & right (C, D) upper eyelids.(A) Prior to epilation. (B) 3 months post-epilation and administration of normal saline (eyelash-regrowth was observed). (C) Prior to epilation. (D) 3 months post-epilation and administration of 0.04% mitomycin C solution (eyelash-regrowth was not observed).

Figure 2.

Light microscopy images of vertical section of excised rat eyelid tissue. The number and size of the hair follicle cells was normal in both the right and left eyelids. However, no hair shaft is present in the hair follicle on the right (treated with mitomycin C) (Hematoxylin-eosin, ×200). (A) Hair follicle of the left eyelid: 3 months post-epilation and administration of normal saline. (B) Hair follicle of the right eyelid: 3 months post-epilation and administration of 0.04% MMC solution.

Figure 3.

Electron micrographs of the eyelid from rats. (A) Normal mitochondria are visible in the hair follicle of the left eyelid (saline-instilled). (B) Mitochondrial destruction is visible in the hair follicle of the right eyelid (mitomycin C-in-stilled). ^∗ Mitochondria.

Table 1.

Number of long eyelashes on the upper eyelid

Rat	Number of eyelashes				Rate of regrowth (%)
Rat	Rt₀	Lt₀	Rt₃	Lt₃	Rt	Lt
1	12	11	1	11	8.33	100
2	16	14	2	13	12.5	92.86
3	11	12	1	12	9.09	100
4	13	12	0	12	0	100
5	15	14	2	14	13.33	100
6	13	13	1	13	7.69	100
7	12	14	1	14	8.33	100
8	14	13	2	13	14.29	100
9	12	12	0	11	0	91.67
10	11	12	0	12	0	100
Mean ± SD	12.9 ± 1.66	12.7 ± 1.05	1.00 ± 0.81	12.5 ± 1.08	7.36 ± 5.55	98.45 ± 3.27

Rt₀ = prior to epilation right; Lt₀ = prior to epilation left; Rt₃ = 3 months post-epilation with 0.04% mitomycin C right; Lt₃ = 3 months post-epilation with normal saline left.

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