Journal List > J Korean Ophthalmol Soc > v.52(10) > 1008901

Kang, Kim, and Lee: The Measurements of Macular Thickness and Volume with SD-OCT in Normal Eyes

Abstract

Purpose

We investigated reproducibility and repeatability of average macular thickness and volume measurements in normal eyes with Cirrus HD OCT (optical coherence tomography).

Methods

Fifty normal eyes from twenty-five subjects without medical and ocular histories were included. Macular cube 512 × 128 combination scanning using the Cirrus HD OCT was performed for a total of three times on the same visit by an experienced technician. Then other two technicians performed one more macular scanning respectively. Within-results, the intraclass correlation coefficient (ICC) was calculated for each parameter studied to evaluate repeatability and reproducibility. The correlation between macular measurements and demographic variables (age, gender, and spherical equivalent) were also investigated.

Results

The ICCs for intraoperator reproducibility were 0.99 on the average macular thickness and 0.96 on the macular volume. And the ICCs for interoperator repeatability were found to be 0.98 and 0.96, respectively. The ICCs for measurements of nine regional retinal thickness also were higher than 0.90. The retinal thickness was correlated with age, gender and refractive error. However, neither age nor refractive error affected to reproducibility and repeatability.

Conclusions

The retinal thickness and macular volume measurements using Cirrus HD OCT in healthy volunteers showed excellent reproducibility and repeatability. Therefore, Cirrus HD OCT has been recognized as an useful tool for diagnosis and mornitoring of variable maculopathies.

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Figure 1.
Standard output of Cirrus OCT, 512 × 128 macular cube (version 3.0.0.64, Carl Zeiss Meditec).
jkos-52-1182f1.tif
Figure 2.
The macular thickness map of the right eye. F = fovea; S1 = superior inner; T1 = temporal inner; I1 = inferior inner; N1 = nasal inner; S2 = superior outer; T2 = temporal outer; I2 = inferior outer; N2 = nasal outer.
jkos-52-1182f2.tif
Table 1.
Demographics
Demographics Results
Age (mean ± SD, yr [range]) 34.32 ± 9.30 (24.00∼61.00)
  <35 years 13/25 (52%)
  ≥35 years 12/25 (48%)
Sex (M/F) 8/17
BCVA* (snellen) 1.0
Refractive error (SE, mean ± SD, diopter [range]) −2.97 ± 2.59 (−9.13∼+1.13)
IOP (mean ± SD, mm Hg [range]) 14.54 ± 2.34 (11.00∼20.00)

* BCVA = best corrected visual acuity

SE = spherical equivalent.

Table 2.
Thickness and volume measurements
Measurements Results (mean ± SD)
Foveal thickness (μ m) 256 ± 18.25
Average macular thickness (μ m) 279.64 ± 12.71
Total macular volume (mm2) 10.03 ± 0.48
Thickness measurements (μ m)
 S1 (superior inner) 320.83 ± 15.93
 T1 (temporal inner) 306.78 ± 14.64
 I1 (inferior inner) 314.02 ± 16.27
 N1 (nasal inner) 323.44 ± 18.34
 S2 (superior outer) 278.49 ± 12.60
 T2 (temporal outer) 260.09 ± 18.24
 I2 (inferior outer) 267.96 ± 16.90
 N2 (nasal outer) 297.83 ± 19.23
Table 3.
ICCs and 95% limits of agreements of intraoperator results
Measurements ICC* 95% limits of agreement
Foveal thickness 0.98 0.96∼0.99
Average macular thickness 0.99 0.98∼0.99
Total macular volume 0.96 0.94∼0.97
Thickness measurements
 S1 (superior inner) 0.98 0.97∼0.99
 T1 (temporal inner) 0.98 0.97∼0.99
 I1 (inferior inner) 0.98 0.97∼0.99
 N1 (nasal inner) 0.98 0.97∼0.99
 S2 (superior outer) 0.93 0.89∼0.96
 T2 (temporal outer) 0.97 0.95∼0.98
 I2 (inferior outer) 0.97 0.95∼0.98
 N2 (nasal outer) 0.96 0.94∼0.98

* ICC = intraclass correlation coefficient.

Table 4.
ICCs and 95% limits of agreements of interoperator results
Measurements ICC* 95% limits of agreement
Foveal thickness 0.96 0.93∼0.97
Average macular thickness 0.99 0.98∼0.99
Total macular volume 0.96 0.94∼0.98
Thickness measurements
 S1 (superior inner) 0.98 0.96∼0.99
 T1 (temporal inner) 0.97 0.95∼0.98
 I1 (inferior inner) 0.96 0.94∼0.98
 N1 (nasal inner) 0.98 0.97∼0.99
 S2 (superior outer) 0.95 0.92∼0.97
 T2 (temporal outer) 0.95 0.93∼0.97
 I2 (inferior outer) 0.96 0.93∼0.98
 N2 (nasal outer) 0.98 0.97∼0.99

* ICC = intraclass correlation coefficient.

Table 5.
Comparision of reproducibility (upper) and repeatability (lower) by age and spherical equivalent
Foveal thickness Average macular thickness Total macular volume
Age < 35 years 0.96 (0.92∼0.98) 0.99 (0.98∼0.99) 0.97 (0.95∼0.99)
Age ≥ 35 years 0.96 (0.91∼0.98) 0.96 (0.92∼0.98) 0.97 (0.94∼0.99)
SE* < −5.0 diopter 0.97 (0.95∼0.98) 0.98 (0.97∼0.99) 0.96 (0.93∼0.98)
SE* ≥ −5.0 diopter 0.91 (0.74∼0.98) 1.00 (0.99∼1.00) 0.99 (0.97∼1.00)
Foveal thickness Average macular thickness Total macular volume
Age < 35 years 0.97 (0.94∼0.99) 0.99 (0.98∼1.00) 0.96 (0.92∼0.98)
Age ≥ 35 years 0.98 (0.96∼0.99) 0.98 (0.96∼0.99) 0.97 (0.94∼0.99)
SE* < −5.0 diopter 0.98 (0.96∼0.99) 0.99 (0.98∼0.99) 0.96 (0.94∼0.98)
SE* ≥ −5.0 diopter 0.97 (0.93∼0.99) 1.00 (0.99∼1.00) 0.97 (0.91∼0.99)

Data presented as intraclass correlation coefficient (95% limit of agreement).

* SE = spherical equivalent.

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