Effects of Pupil Dilation and Constriction Agents on Trabecular Meshwork Cells

Jeong Hun Lee; Jae Woo Kim

doi:10.3341/jkos.2011.52.9.1089

Journal List > J Korean Ophthalmol Soc > v.52(9) > 1008884

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Lee and Kim: Effects of Pupil Dilation and Constriction Agents on Trabecular Meshwork Cells

Original Article

J Korean Ophthalmol Soc 2011;52(9):1089-1093.

Published online: 7 September 2011

DOI: https://doi.org/10.3341/jkos.2011.52.9.1089

Effects of Pupil Dilation and Constriction Agents on Trabecular Meshwork Cells

Jeong Hun Lee, MD, Jae Woo Kim, MD, PhD

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea

Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Daegu Catholic University Medical Center #3056-6 Daemyeong 4-dong, Nam-gu, Daegu 705-718, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133, E-mail: jwkim@cu.ac.kr

Received 17 February 2011 Revised 19 April 2011 Accepted 12 July 2011

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the effects of pupil dilation and constriction agents on the survival and production of nitric oxide (NO) in cultured human trabecular meshwork cells (HTMC).

Methods

Primarily cultured HTMC were exposed to 0, 0.01, and 0.1 mg/ml of tropicamide, cyclopentolate, atropine, or pilocarpine for 2 hours. Cellular survival and production of NO were assessed using the MTT assay and Griess assay, respectively.

Results

Tropicamide, cyclopentolate, atropine, and pilocarpine decreased cellular survival at the concentration of 0.1 mg. At the concentration of 0.01 mg/ml, all agents decreased production of NO to some extent, although the reduction was not statistically significant.

Conclusions

Pupil dilation and constriction agents may be toxic to HTMC if used at high concentrations or if used frequently in the short-term but may not affect trabecular outflow.

Keywords: Atropine, Cyclopentolate, Pilocarpine, Trabecular meshwork cells, Tropicamide

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Figure 1.

Effects of tropicamide on the survival of trabecular meshwork cells. Tropicamide decreased cellular survival. ^* p < 0.05.

Figure 2.

Effects of cyclopentolate on the survival of trabecular meshwork cells. Cyclopentolate decreased cellular survival. ^* p < 0.05.

Figure 3.

Effects of atropine on the survival of trabecular meshwork cells. Atropine decreased cellular survival. ^* p < 0.05.

Figure 4.

Effects of pilocarpine on the survival of trabecular meshwork cells. Pilocarpine decreased cellular survival. ^* p < 0.05.

Figure 5.

Effects of 0.01 mg/ml tropicamide, cyclopentolate, atropine, and pilocarpine on the production of nitric oxide in trabecular meshwork cells. All agents did not affect the production of nitric oxide significantly compared to the non-exposed control. p > 0.05.

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