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Abstract
Purpose
The effect of 0.2% cyclosporin A (CsA) as an adjuvant therapy after glaucoma-filtering surgery was the focus of this study.
Methods
A posterior lip sclerotomy was performed in 16 eyes of 8 rabbits, and 0.2% CsA was administered into the right eyes. The left eyes served as controls. The intraocular pressure (IOP) was measured 1, 3, 5, 7, 14, and 28 days after surgery. Hematoxylin-eosin (HE) and anti-bromodeoxyuridine (BrdU) immunocytochemical staining were performed at 1, 2, 4, and 8 weeks.
Results
The IOP at 7 and 14 days after surgery was lower in the 0.2% CsA group and statistically significant (P=0.047, P=0.48; respectively). HE staining did not show any difference between experimental and control eyes, but anti-BrdU staining showed a lower number of positive cells in the experimental eyes at 1 week. The fibroblast proliferation rate was significantly lower 1 week after surgery in the 0.2% CsA group (P=0.003).
References
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Figure 1.
Glaucoma filtering surgery in a rabbit eye. A fornix-based conjunctival incision was undertaken in the upper-nasal part of the eye (between superior rectus muscle and medial rectus muscle). The anterior chamber was then entered through the filtering site, and a 3×1 mm block of scleral tissue and trabecular meshwork was excised. Peripheral iridectomy was not performed.
Figure 2.
High magnification photographs of immunocytochemically stained sections (×400). The right photographs are eyes instilled with 0.2% CsA (A). The left photographs are control eyes (B). Many bromodeoxyuridine (BrdU)-positive cells (red arrow) are shown 1 week after surgery (A and B). Fewer BrdU-positive cells existed in experimental eyes (A). The scale bars indicate 50 μm.
Table 1.
Mean intraocular pressure between two groups (Mean± SD)
Table 2.
Proliferation rates of fibroblasts after BrdU injection in experimental and control eyes (Means± SDs*)
| Days |
Proliferation rate (%) |
P value | |
|---|---|---|---|
| with 0.2% CsA | without 0.2% CsA | ||
| 1 wk | 20.17±1.37 | 30.83±0.22 | .003 |
| 2 wk | 6.92±1.24 | 9.47±1.96 | .138 |
| 1 mo | 2.90±0.60 | 1.96±0.48 | .138 |
| 2 mo | 1.32±0.04 | 1.47±0.32 | 1.000 |
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