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Journal List > J Korean Ophthalmol Soc > v.51(5) > 1008818

Kim, Hwang, Park, and Moon: Glaucoma Filtering Surgery With Low Concentration of Cyclosporin A in Rabbits: A Pilot Study

Abstract

Purpose

The effect of 0.2% cyclosporin A (CsA) as an adjuvant therapy after glaucoma-filtering surgery was the focus of this study.

Methods

A posterior lip sclerotomy was performed in 16 eyes of 8 rabbits, and 0.2% CsA was administered into the right eyes. The left eyes served as controls. The intraocular pressure (IOP) was measured 1, 3, 5, 7, 14, and 28 days after surgery. Hematoxylin-eosin (HE) and anti-bromodeoxyuridine (BrdU) immunocytochemical staining were performed at 1, 2, 4, and 8 weeks.

Results

The IOP at 7 and 14 days after surgery was lower in the 0.2% CsA group and statistically significant (P=0.047, P=0.48; respectively). HE staining did not show any difference between experimental and control eyes, but anti-BrdU staining showed a lower number of positive cells in the experimental eyes at 1 week. The fibroblast proliferation rate was significantly lower 1 week after surgery in the 0.2% CsA group (P=0.003).

Conclusions

An effect of 0.2% CsA on early wound healing was observed. The data suggest that a low concentration of CsA can be useful when employed as adjuvant therapy in glaucoma filtering surgery.

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References

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jkos-51-740f1.tif
Figure 1.
Glaucoma filtering surgery in a rabbit eye. A fornix-based conjunctival incision was undertaken in the upper-nasal part of the eye (between superior rectus muscle and medial rectus muscle). The anterior chamber was then entered through the filtering site, and a 3×1 mm block of scleral tissue and trabecular meshwork was excised. Peripheral iridectomy was not performed.
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jkos-51-740f2.tif
Figure 2.
High magnification photographs of immunocytochemically stained sections (×400). The right photographs are eyes instilled with 0.2% CsA (A). The left photographs are control eyes (B). Many bromodeoxyuridine (BrdU)-positive cells (red arrow) are shown 1 week after surgery (A and B). Fewer BrdU-positive cells existed in experimental eyes (A). The scale bars indicate 50 μm.
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Table 1.
Mean intraocular pressure between two groups (Mean± SD)
Days Rabbits Intraocular pressure (mm Hg)
P value
with 0.2% CsA without 0.2% CsA
Baseline 8 17.66±1.76 17.76±2.94 .999
1 8 15.13±3.45 15.19±2.75 .888
3 8 15.25±4.01 16.06±4.61 .574
5 8 14.63±2.33 16.63±2.05 .060
7 8 15.44±2.74 18.06±3.17 .047
14 6 15.00±2.74 16.83±2.21 .048
28 4 14.00±1.78 14.63±1.80 .465

Wilcoxon signed-Rank test.

Table 2.
Proliferation rates of fibroblasts after BrdU injection in experimental and control eyes (Means± SDs*)
Days Proliferation rate (%)
P value
with 0.2% CsA without 0.2% CsA
1 wk 20.17±1.37 30.83±0.22 .003
2 wk 6.92±1.24 9.47±1.96 .138
1 mo 2.90±0.60 1.96±0.48 .138
2 mo 1.32±0.04 1.47±0.32 1.000

* Standard deviations represented for 3 high powered field counts.

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