Role of Nitric Oxide in the Proliferative and Migratory Effect of Triamcinolone in RPE Cells

Jae Woo Kim; Jae Hyung Lee; Seung Hee Lee

doi:10.3341/jkos.2010.51.1.120

Journal List > J Korean Ophthalmol Soc > v.51(1) > 1008636

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Kim, Lee, and Lee: Role of Nitric Oxide in the Proliferative and Migratory Effect of Triamcinolone in RPE Cells

Original Article

J Korean Ophthalmol Soc 2010;51(1):120-125.

Published online: 7 September 2010

DOI: https://doi.org/10.3341/jkos.2010.51.1.120

Role of Nitric Oxide in the Proliferative and Migratory Effect of Triamcinolone in RPE Cells

Jae Woo Kim, MD, PhD, Jae Hyung Lee, MD, Seung Hee Lee, MD

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea

Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Catholic University of Daegu College of Medicine #3056-6 Daemyeung-4-dong, Nam-gu, Daegu 705-718, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133, E-mail: jwkim@cu.ac.kr

Received 3 April 2009 Accepted 15 September 2009

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the role of nitric oxide (NO) on the proliferative and migratory effects of triamcinolone acetonide (TA) in retinal pigment epithelial cells.

Methods

After exposure to 10 nM, 1 μ M, or 100 μ M TA for four days, with or without co-exposure of antioxidant N-acetylcyteine, the proliferation and nitrite production of ARPE19 cells were assessed with MTT and Griess assays, respectively. Additionally, a cell migration assay was performed.

Results

Cellular survival increased after exposure to TA at low concentration but decreased at high concentration. TA decreased the production of NO and cellular migration significantly, and these effects were abolished by N-acetylcysteine. Conclusions: TA showed a biphasic response on the proliferation and decreased cellular migration in ARPE19 cells, which may be mediated by nitric oxide.

Keywords: ARPE19, Migration, Nitric oxide, Triamcinolone acetonide

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Figure 1.

Effect of triamcinolone acetonide (TA) on the proliferation of ARPE19 cells exposed for 4 days. TA exhibited biphasic response and N-acetyl cysteine (NAC) increased proliferation (^*, ^**; p<0.05).

Figure 2.

Effect of triamcinolone acetonide (TA) on the migration of ARPE19 cells. TA inhibited cellular migration sighnificantly (^*; p<0.05) and co-exposure of 2 mM N-acetyl cysteine (NAC) abolished TA-induced inhibition of cellular migration (^**; p<0.05).

Figure 3.

Effect of triamcinolone acetonide (TA) on the production of nitric oxide exposed for 4 days. Co-exposure of 200 μM N-acetyl cysteine (NAC) further abolished TA-induced inhibition of nitric oxide production (^*; p<0.05).

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