Effect of Bevacizumab on Survival and Production of Nitric Oxide in Trabecular Meshwork Cells

Sin Hoo Kim; Jae Woo Kim

doi:10.3341/jkos.2009.50.10.1404

Journal List > J Korean Ophthalmol Soc > v.50(9) > 1008370

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Kim and Kim: Effect of Bevacizumab on Survival and Production of Nitric Oxide in Trabecular Meshwork Cells

Original Article

J Korean Ophthalmol Soc 2009;50(9):1404-1408.

Published online: 7 September 2009

DOI: https://doi.org/10.3341/jkos.2009.50.10.1404

Effect of Bevacizumab on Survival and Production of Nitric Oxide in Trabecular Meshwork Cells

Sin Hoo Kim, MD, Jae Woo Kim, MD, PhD

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea

Address reprint requests to Jae Woo Kim, MD, PhD Department of Ophthalmology, Catholic University of Daegu College of Medicine #3056-6 Daemyeung 4-dong, Nam-gu, Daegu 705-718, Korea Tel: 82-53-650-4728, Fax: 82-53-627-0133, E-mail: jwkim@cu.ac.kr

Received 21 April 2009 Accepted 23 June 2009

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the effect of bevacizumab, a monoclonal antibody against vascular endothelial growth factor (VEGF), on the survival and production of nitric oxide (NO) in human trabecular meshwork cells (HTMC).

Methods

Primarily cultured HTMC were exposed to 0, 0.25, 1.0, and 2.5 mg/ml bevacizumab for 24 hours. Cellular survival and production of NO were assessed by MTT assay and Griess assay, respectively.

Results

Bevacizumab did not affect the cellular survival at low concentrations but decreased cellular survival significantly at high concentrations (>1.0 mg/ml) accompanied with increased NO production.

Conclusions

High concentrations of bevacizumab may be toxic to HTMC.

Keywords: Bevacizumab, Nitric oxide, Trabecular meshwork cells

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Figure 1.

Bevacizumab decreased survival of trabecular meshwork cells significantly from 0.5 mg/ml and decreased markedly from 1.0 mg/ml of concentration. (^∗ p<0.05)

Figure 2.

Effect of L-NAME on the bevacizumab-induced decreased survival of trabecular meshwork cells. 0.5 mM L-NAME did not affect the survival significantly at high concentration. (^∗ p<0.05)

Figure 3.

Bevacizumab decreased NO production at lower concentrations but significantly increased at high concentrations in trabecular meshwork cells. (^∗ p<0.05)

Figure 4.

0.5 mM L-NAME did not affect the bevacizumab-induced production of nitric oxide significantly at high concentrations in trabecular meshwork cells. (^∗ p<0.05)

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