Journal List > J Korean Ophthalmol Soc > v.49(10) > 1008110

Woo Kim, Hoo Kim, and Hyung Lee: Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells



To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence.


Primary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-β-gal assays, respectively.


Hydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence.


Oxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma.


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Figure 1.
Effect of hydrogen-peroxide-induced oxidative stress on the production of NO in cultured trabecular meshwork cells. Hydrogen peroxide inhibited NO production significnatly, which was abolished by co-exposed L-ascorbic acid (LAA) (* p<0.05).
Figure 2.
Effect of hydrogen peroxide on the survival of trabecular meshwork cells. Hydrogen peroxide decreased cellular survival significantly in a dose dependent manner. Co-exposed 0.1 mM L-ascorbic acid did not affect on the survival (* p<0.05).
Figure 3.
Photograph of SA-β-gal positive senescent trabecular meshwork cells (blue color) after exposed to 0.05 mM of hydrogen peroxide. Original magnification ×100.
Figure 4.
Effect of hydrogen peroxide on the induction of cellular senescence in cultured trabecular meshwork cells. Hydrogen peroxide increased cellular senescence significantly, which was not abolished by co-exposed 0.1 mM L-ascorbic acid (* p<0.05).
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