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Journal List > J Korean Ophthalmol Soc > v.49(9) > 1008068

Kim, Kim, Ryu, Chun, You, Yoon, and Kim: The Effect of an Anti-oxidative and Anti-inflammatory Functional Dietary Supplement in Dry Eye Syndrome

Abstract

Purpose

To evaluate the effect of Optibiol®, which is composed of multiple anti-oxidative and anti-inflammatory dietary elements, on the symptoms, clinical manifestations, and expression of various inflammatory cytokines in dry eye patients.

Methods

Patients suffering from dry eye were given Optibiol® for 3 months. They completed questionnaires regarding dry eye symptom and underwent slit lamp biomicroscopic examinations, tear film breakup times, Shirmer tests, and conjunctival fluorescein staining examinations on a monthly basis during the intake of Optibiol®. Sampling of serum and tears was conducted at baseline and 3 months after taking Optibiol®, and various inflammatory cytokines in the serum and tears were measured with multiplex bead immunoassay.

Result

Sixty-three patients were included in this study. After taking Optibiol®, the dry eye symptoms, Schirmer scores, tear film breakup times, and conjunctival staining scores (Oxford scale) showed significant improvement, and the expression of most inflammatory cytokines had decreased: in particular, IL-1? and MIP-1? in serum, and IL-17 and MIP-1? in tears were significantly lower.

Conclusions

Optibiol®, an anti-oxidative and anti-inflammatory functional dietary supplement, is an effective dietary supplement in patients with dry eye syndrome. We posit that the decreased expression of inflammatory cytokines is an important mechanism in this effect.

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jkos-49-1397f1.tif
Figure 1.
Changes in scores of 6 ocular symptoms before and after taking Optibiol®.* p<0.05 : One way ANOVA, Duncan multiple range test.
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jkos-49-1397f2.tif
Figure 2.
Changes in Schirmer score and tear break-up time before and after taking Optibiol®. BUT=tear film breakup time,* p<0.05 : One way ANOVA, Duncan multiple range test.
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jkos-49-1397f3.tif
Figure 3.
Concentration of inflammatory cytokines in serum, before and after taking Optibiol®. After taking Optibiol®, the expression of most inflammatory cytokines had decreased in serum, especially IL-1β and MIP-1β were significantly lower; IL=interleukin; TNF-α=tumor necrosis factor-α; MIP-1β=macrophage inflammatory protein-1β; MCP-1=monocyte chemoattractant protein-1; * p<0.05 : Student T-test.
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jkos-49-1397f4.tif
Figure 4.
According to age groups, changes of inflammatory cytokines in serum after taking Optibiol®. No significant difference in changes of inflammatory cytokines in serum after taking Optibiol®, according to age groups; IL=interleukin; TNF-α=tumor necrosis factor-α; MIP-1β=macrophage inflammatory protein-1β; MCP-1=monocyte chemoattractant protein-1.
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jkos-49-1397f5.tif
Figure 5.
Changes of inflammatory cytokines in serum according to sex after taking Optibiol®. No significant difference in changes of inflammatory cytokines in serum after taking Optibiol®, according to sex. IL=interleukin; TNF-α=tumor necrosis factor-α; MIP-1β=macrophage inflammatory protein-1β; MCP-1=monocyte chemoattractant protein-1.
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jkos-49-1397f6.tif
Figure 6.
Concentrations of inflammatory cytokines in tear, before and after taking Optibiol®. After taking Optibiol®, the expression of all inflammatory cytokines had decreased in tear, especially IL-17 and MIP-1β were significantly lower. IL=interleukin; G-CSF=granulocyte colony stimulating factor; MIP-1β=macrophage inflammatory protein-1β.
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jkos-49-1397f7.tif
Figure 7.
Changes of concentrations of inflammatory cytokines in tear according to age groups and sex after taking Optibiol®. No significant difference in changes of inflammatory cytokines in serum after taking Optibiol®, according to age groups and sex. IL=interleukin; G-CSF=granulocyte colony stimulating factor; MIP-1β=macrophage inflammatory protein-1β.
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Table 1.
Nutritional facts in 1 capsule of Optibiol®
Nutritional value For 1 soft capsule
Protein 0.37 g
Glucoside 0.09 g
Lipids 440 mg
Saturated fatty acids 90 mg
Eicosapentanoic acid (EPA) 41 mg
Docosahexanoic acid (DHA) 27 mg
Cartenoids 580 mg
Lutein 10 mg
Zeaxanthin 520 µg
Cryptoxanthin 50 µg
Vitamin E 10 mg
Vitamin C 9 mg
Chrome 25 µg
Zinc 15 mg
Energy 5 kcal / 21 kJ
Table 2.
Changes of conjunctival staining scores and serum lipid level before and after taking Optibiol
Baseline After 3 months p-value
Conjunctival stain score (OD) 1.48 0.94 0.000
Conjunctival stain score (OS) 1.48 0.98 0.000
Total cholesterol 182.77 mg/dl 182.49 mg/dl 0.939
Triglyceride 130.93 mg/dl 133.02 mg/dl 0.818
Low density lipoprotein (LDL) 114.63 mg/dl 110.46 mg/dl 0.217
High density lipoprotein (HDL) 55.76 mg/dl 59.42 mg/dl 0.018

student T-test.

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