Effects of Intravitreal Amniotic Membrane Injection in a Rat Model of Choroidal Neovascularization

Jong Hyun Lee; Jae Lim Chung; Samin Hong; Hyoung Jun Koh

doi:10.3341/jkos.2008.49.7.1154

Journal List > J Korean Ophthalmol Soc > v.49(7) > 1008026

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Hyun Lee, Chung, Hong, and Koh: Effects of Intravitreal Amniotic Membrane Injection in a Rat Model of Choroidal Neovascularization

Original Article

J Korean Ophthalmol Soc 2008;49(7):1154-1158.

Published online: 7 September 2008

DOI: https://doi.org/10.3341/jkos.2008.49.7.1154

Effects of Intravitreal Amniotic Membrane Injection in a Rat Model of Choroidal Neovascularization

Jong Hyun Lee, M.D.¹, Jae Lim Chung, M.D.², Samin Hong, M.D.², Hyoung Jun Koh, M.D.^2,

Department of Ophthalmology, Ilsan Paik Hospital, Inje University, Goyang-si, Korea

Department of Ophthalmology, Yonsei University College of Medicine, The Institute of Vision Research, Seoul, Korea

Address reprint requests to Hyoung Jun Koh, M.D. Department of Ophthalmology, Severance Hospital, College of Medicine, Yonsei University #134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Korea Tel: 82-2-2228-3570, Fax: 82-2-312-0541, E-mail: hjkoh@yuhs.ac

Received 18 March 2008 Accepted 28 June 2007

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To evaluate the effects of an intravitreal amniotic membrane (AM) injection on a rat model of laser-induced choroidal neovascularization (CNV).

Methods

Intravitreal injection of AM (12.5 µg/mL) into the left eyes and phosphate-buffered saline into the fellow eyes of 15 rats was performed immediately after laser injury. At week 2, after angiography, the rats were sacrificed, and histopathologic analysis was performed. Angiographic and histopathologic assessments of the effects of AM injection on CNV growth were compared between the two groups.

Results

CNV thickness was found to have increased more in the AM treatment group (141.54±8.12 µm) than in the experimental control group (65.23±16.90 µm) (p=0.010).

Conclusions

Intravitreal AM injection demonstrated histopathologic pro-angiogenic properties in a rat model of CNV.

Keywords: Amniotic membrane, Angiogenesis, Choroidal neovascularization

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Figure 1.

Histopathologic sections in hematoxylin-eosin (H&E) staining showing choroidal neovascularization induced by laser. (A) Amniotic membrane treatment group; (B) Experimental control group (400 times magnification, high power field view, light microscope).

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