Effect of Nitric Oxide on the Trabecular Meshwork Cell-mediated Contraction of Collagen Gels

Jae Woo Kim; Woo Seok Chang; Su Yoon Lee

doi:10.3341/jkos.2007.48.11.1548

Journal List > J Korean Ophthalmol Soc > v.48(11) > 1007969

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Kim, Chang, and Lee: Effect of Nitric Oxide on the Trabecular Meshwork Cell-mediated Contraction of Collagen Gels

Original Article

J Korean Ophthalmol Soc 2007;48(11):1548-1553.

Published online: 28 August 2007

DOI: https://doi.org/10.3341/jkos.2007.48.11.1548

Effect of Nitric Oxide on the Trabecular Meshwork Cell-mediated Contraction of Collagen Gels

Jae Woo Kim, M.D., Woo Seok Chang, M.D., Su Yoon Lee, M.D.

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea

Address reprint requests to Jae Woo Kim, M.D., Ph.D., Department of Ophthalmology, Catholic University of Daegu School of Medicine, #3056-6 Daemyeung-4-dong, Nam-gu, Daegu 705-718, Korea, Tel: 82-53-650-4728, Fax: 82-53-627-0133, E-mail: jwkim@cu.ac.kr

Received 23 April 2007 Accepted 14 August 2007

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

To investigate the effect of nitric oxide (NO) on the contraction of cultured human trabecular meshwork cells (HTMCs).

Methods

After embedding them into collagen gels, primarily cultured HTMCs were exposed to NO donors, such as sodium nitroprusside (SNP) or S-Nitroso-N-acetylpenicillamine (SNAP), for 1 week at various concentrations, and the contraction of the collagen gels was measured. Cellular survival and NO production were measured with MTT assay and Griess assay, respectively.

Results

Though SNP and SNAP did not significantly affect cellular survival, they markedly enhanced NO production. Both sodium nitroprusside and SNAP inhibited the contraction of collagen gels by about 10% in dose and time-dependent manners (p<0.05).

Conclusions

NO donors inhibited the contraction of collagen gels in vitro. Thus, NO donors may relax trabecular meshwork and enhance trabecular outflow.

Keywords: Collagen gel, Contraction, Nitric oxide, Trabecular meshwork

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Figure 1.

Effect of NO dcncrs (μM) cn the survival of trabecular meshwcrk cells embedded in the collagen gel measured with MTT assay. L-NAME and NO dcncrs did not affect cn the survival. (p>0.05)

Figure 2.

Effect cf NO cbncrs (μM) cn the prcducticn cf NO measured with Griess assay. SNP and SNAP increased NO prcducticn significantly. (*; p<0.05)

Figure 3.

Effect cf scdium nitrcprusside (SNP) cn the ccntracticn cf ccllagen gels. SNP inhibited the ccntracticn cf ccllagen gels significantly. (*; p<0.05, 10 μM) (jkos-48-1548; p<0.05, 100μM)

Figure 4.

Effect cf SNAP cn the ccntracticn cf cc lagen gels. SNAP inhibited the ccntracticn cf ccllagen gels significantly in a time- and dcse-dependent manner. (*; p<0.05, 1μM) (jkos-48-1548; p<0.05, 10μM)

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